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Delineating The Skeletal Muscle-Microvessel Regeneration Program After Ischemic Injury, Jason J. Lee

Electronic Thesis and Dissertation Repository

Understanding the cellular processes involved in skeletal muscle regeneration after damage is critical to advancing translational efforts toward the development of targeted therapeutics. The purpose of this thesis was to ascertain the dynamic interplay between regenerating muscle and regenerating blood vessels after ischemic injury.

First, using a novel systematic investigation of a widely used preclinical mouse model of limb ischemia, I generated a detailed atlas of muscle injury zones and angiogenesis zones in C57BL/6 mice subjected to femoral artery excision. This uncovered previously unrecognized regional variability and an exclusive relationship between angiogenesis and actively regenerating muscle zones. Then, an unbiased, …