Medicine and Health Sciences Commons^™

Analysis Of Genetic Diversity Using Simple Sequence Repeat (Ssr) Markers And Growth Regulator Response In Biofield Treated Cotton (Gossypium Hirsutum L.), Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana

Mahendra Kumar Trivedi

Cotton is the most important crop for the production of fiber that plays a key role in economic and social affairs. The aim of the study was to evaluate the impact of biofield energy treatment on cotton seeds regarding its growth, germination of seedling, glutathione (GSH) concentration, indole acetic acid (IAA) content and DNA fingerprinting using simple sequence repeat (SSR) markers for polymorphism analysis. The seeds of cotton cv. Stoneville-2 (Gossypium hirsutum L.) was obtained from DNA Land Marks Inc., Canada and divided into two groups. One group was remained as untreated, while the other was subjected to Mr. Trivedi …

Antimicrobial Susceptibility, Biochemical Characterization And Molecular Typing Of Biofield Treated Klebsiella Pneumoniae, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Mayank Gangwar, Snehasis Jana

Mahendra Kumar Trivedi

Characterization Of Phenotype And Genotype Of Biofield Treated Enterobacter Aerogenes, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana

Mahendra Kumar Trivedi

Genetics Of Plasminogen Activator Inhibitor-1 (Pai-1) In A Ghanaian Population, Marquitta J. White, Nuri M. Kodaman, Reed H. Harder, Folkert W. Asselbergs, Douglas E. Vaughan, Nancy J. Brown, Jason H. Moore, Scott M. Williams

Dartmouth Scholarship

Plasminogen activator inhibitor 1 (PAI-1), a major modulator of the fibrinolytic system, is an important factor in cardiovascular disease (CVD) susceptibility and severity. PAI-1 is highly heritable, but the few genes associated with it explain only a small portion of its variation. Studies of PAI-1 typically employ linear regression to estimate the effects of genetic variants on PAI-1 levels, but PAI-1 is not normally distributed, even after transformation. Therefore, alternative statistical methods may provide greater power to identify important genetic variants. Additionally, most genetic studies of PAI-1 have been performed on populations of European descent, limiting the generalizability of their …

Genome-Wide Meta-Analysis In Alopecia Areata Resolves Hla Associations And Reveals Two New Susceptibility Loci, Regina C. Betz, Lynn Petukhova, Stephan Ripke, Hailiang Huang, Androniki Menelaou, Silke Redeler, Tim Becker, Stefanie Heilmann, Tarek Yamany, Madeleine Duvic, Maria Hordinsky, David Norris, Vera H. Price, Julian Mackay-Wiggan, Annemieke De Jong, Gina M. Destefano, Susanne Moebus, Markus Böhm, Ulrike Blume-Peytavi, Hans Wolff, Gerhard Lutz, Roland Kruse, Li Bian, Christopher I. Amos

Dartmouth Scholarship

Alopecia areata (AA) is a prevalent autoimmune disease with ten known susceptibility loci. Here we perform the first meta-analysis in AA by combining data from two genome-wide association studies (GWAS), and replication with supplemented ImmunoChip data for a total of 3,253 cases and 7,543 controls. The strongest region of association is the MHC, where we fine-map 4 independent effects, all implicating HLA-DR as a key etiologic driver. Outside the MHC, we identify two novel loci that exceed statistical significance, containing ACOXL/BCL2L11(BIM) (2q13); GARP (LRRC32) (11q13.5), as well as a third nominally significant region SH2B3(LNK)/ ATXN2 (12q24.12). Candidate susceptibility gene expression …

Whole Genome Capture Of Vector-Borne Pathogens From Mixed Dna Samples: A Case Study Of Borrelia Burgdorferi, Giovanna Carpi, Katharine S. Walter, Stephen J. Bent, Anne Gatewood Hoen, Maria Diuk-Wasser, Adalgisa Caccone

Dartmouth Scholarship

Background:

Rapid and accurate retrieval of whole genome sequences of human pathogens from disease vectors or animal reservoirs will enable fine-resolution studies of pathogen epidemiological and evolutionary dynamics. However, next generation sequencing technologies have not yet been fully harnessed for the study of vector-borne and zoonotic pathogens, due to the difficulty of obtaining high-quality pathogen sequence data directly from field specimens with a high ratio of host to pathogen DNA.

Results:

We addressed this challenge by using custom probes for multiplexed hybrid capture to enrich for and sequence 30 Borrelia burgdorferi genomes from field samples of its arthropod vector. Hybrid …

Determination Of The Alui Polymorphism Effect Of Bovine Growth Hormone Gene On Carcass Traits In Zavot Cattle With Analysis Of Covariance, Aytaç Akçay, Bi̇lal Akyüz, Davut Bayram

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the genotypes of bovine growth hormone and to investigate their association with daily weight gain in the Zavot cattle breed. The bovine growth hormone (GH) genotypes of 45 male Zavot cattle were identified by PCR-RFLP. The effect of the bovine growth hormone gene on daily weight gain was estimated from a dataset obtained from cattle up to the age of 9 months. The mean differences of carcass weight or carcass yield among the genotype groups were assessed by analysis of covariance (ANCOVA). Two alleles for bovine growth hormone locus, L and V, …

Polymorphisms Of Ovine Prion Protein (Prp) Gene In Pramenka Sheepbreed Population In Bosnia And Herzegovina, Ervin Zecevic, Admir Dokso, Amra Kazic, Muhamed Brka

Turkish Journal of Veterinary & Animal Sciences

Transmissible spongiform encephalopathies (TSEs) are a group of diseases that affect the nervous system and lead to death. The putative infectious agent is the host-encoded prion protein (PrP) and it appears that the development of scrapie in sheep is closely related to polymorphisms in the host PrP gene. The aim of this study was to investigate three well-known polymorphisms in exon 3 of the PrP gene (at codons 136, 154, and 171) that have a clear and significant effect on scrapie in sheep. Four other polymorphisms (at codons 145, 185, 231, and 237) that are yet undefined were also investigated. …