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Full-Text Articles in Medicine and Health Sciences
Scardovia Wiggsiae Prevalence In Orthodontic Patients, Brandon Joseph Streiff
Scardovia Wiggsiae Prevalence In Orthodontic Patients, Brandon Joseph Streiff
UNLV Theses, Dissertations, Professional Papers, and Capstones
Dental caries has mainly been associated with Streptococcus, Lactobacillus, Actinomyces and Veillonella species. But in recent salivary studies a new cariogenic pathogen, has been identified. This new bacteria, Scardovia wiggsiae (SW), is currently being tested within the UNLV School of Dental Medicine patient population. Although these current studies are being conducted to study its prevalence in both pediatric and adult populations, it has not been evaluated among patients with an altered oral environment as seen in patients with orthodontic appliances. Fixed orthodontic appliances increase the difficulty of removing daily plaque on and in between the teeth with standard oral hygiene …
Engineering Novel Detection And Treatment Strategies For Bacterial Therapy Of Cancer, Jan T. Panteli
Engineering Novel Detection And Treatment Strategies For Bacterial Therapy Of Cancer, Jan T. Panteli
Doctoral Dissertations
Finding and treating cancer is difficult due to limited sensitivity and specificity of current detection and treatment strategies. Many chemotherapeutic drugs are small molecules that are limited by diffusion, making it difficult to reach cancer sites requiring high doses that lead to systemic toxicity and off-target effects. Tomographic detection techniques, like PET, MRI and CT, are good at identifying macroscopic lesions in the body but are limited in their ability to detect microscopic lesions. Biomarker detection strategies are extremely sensitive and able to identify ng/ml concentrations of protein, but are poor at discriminating between healthy and disease state levels due …
Application Of Β-Lactamase Reporter Fusions As An Indicator Of Effector Protein Secretion During Infections With The Obligate Intracellular Pathogen Chlamydia Trachomatis , Konrad E. Mueller, Kenneth A. Fields
Application Of Β-Lactamase Reporter Fusions As An Indicator Of Effector Protein Secretion During Infections With The Obligate Intracellular Pathogen Chlamydia Trachomatis , Konrad E. Mueller, Kenneth A. Fields
Microbiology, Immunology, and Molecular Genetics Faculty Publications
Chlamydia spp. utilize multiple secretion systems, including the type III secretion system (T3SS), to deploy host-interactive effector proteins into infected host cells. Elucidation of secreted proteins has traditionally required ectopic expression in a surrogate T3SS followed by immunolocalization of endogenous candidate effectors to confirm secretion by chlamydiae. The ability to transform Chlamydia and achieve stable expression of recombinant gene products has enabled a more direct assessment of secretion. We adapted TEM-1 β-lactamase as a reporter system for assessment of chlamydial protein secretion. We provide evidence that this system facilitates visualization of secretion in the context of infection. Specifically, our findings …
Comparative Analysis Of Anti-Bd Bacteria From Six Malagasy Frog Species Of Ranomafana National Park, Kelsey Savage
Comparative Analysis Of Anti-Bd Bacteria From Six Malagasy Frog Species Of Ranomafana National Park, Kelsey Savage
Senior Honors Projects, 2010-2019
As Malagasy amphibians are facing an impending extinction crisis from the lethal skin fungus Batrachochytrium dendrobatidis (Bd), it has become imperative to proactively mitigate the threat. Bd sporangia develop in the skin of infected amphibians and cause the skin to thicken, leading to ionic imbalance and eventual heart failure. It has been shown that certain bacterial species are able to inhibit Bd growth on amphibians by producing antifungal metabolites. Community-based probiotics are one approach used to combat chytridomycosis by inoculating an environment with Bd-inhibitory bacteria so that many amphibian species are treated at once. With this method, it is important …
Examining Phage Infection Utilizing Mycobacterium Smegmatis, Tanya L. Riddick
Examining Phage Infection Utilizing Mycobacterium Smegmatis, Tanya L. Riddick
Undergraduate Research
Bacteriophages, also known as phages, are viruses that are ubiquitous and survive and replicate within the host of the bacterial cell, Mycobacterium smegmatis. They are considered one of the most abundant organisms on earth (1031). Structurally, they are 100-200nm in size and consist of a protein encapsulated head that contains DNA or RNA, a tail sheath and tail fibers. This research consisted of examining phage infection, by re-isolating a novel phage, Tango. Tango was originally isolated genetically in 2013 by a previous ISBT student, Anna Maccarrone. The phage was sent to genetic sequencing but two phages were discovered, …