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Full-Text Articles in Medicine and Health Sciences
Intestinal Inflammation Model Inducing Gut Leakage By Different Methods In Broiler Chickens Using Fitc-D As A Marker, Eduardo Vicuna
Intestinal Inflammation Model Inducing Gut Leakage By Different Methods In Broiler Chickens Using Fitc-D As A Marker, Eduardo Vicuna
Graduate Theses and Dissertations
Traditionally, antibiotic growth promoters (AGP) have been used in food animals to reduce enteric inflammation and maintain intestinal homeostasis, thus improving performance. Due to increasing restrictions regarding the use of AGP, precise and high throughput enteric inflammation models and markers to search for effective alternatives are urgently needed. Oral administration of fluorescein isothiocyanate dextran (FITC-d) and its passage into blood can be used as a marker for tight junction permeability. FITC-d is a large molecule (3-5 kDa) which does not usually leak through the intact gastrointestinal tract barrier. However, when conditions disrupt the tight junctions between epithelial cells, the FITC-d …