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Genome-Wide Profiling Of Histone Modifications (H3k9me2 And H4k12ac) And Gene Expression In Rust (Uromyces Appendiculatus) Inoculated Common Bean (Phaseolus Vulgaris L), Vasudevan Ayyappan, Venu Kalavacharla, Jyothi Thimmapuram, Ketaki P. Bhide, Venkateswara R. Sripathi
Genome-Wide Profiling Of Histone Modifications (H3k9me2 And H4k12ac) And Gene Expression In Rust (Uromyces Appendiculatus) Inoculated Common Bean (Phaseolus Vulgaris L), Vasudevan Ayyappan, Venu Kalavacharla, Jyothi Thimmapuram, Ketaki P. Bhide, Venkateswara R. Sripathi
Cyber Center Publications
Histone modifications such as methylation and acetylation play a significant role in controlling gene expression in unstressed and stressed plants. Genome-wide analysis of such stress-responsive modifications and genes in non-model crops is limited. We report the genome-wide profiling of histone methylation (H3K9me2) and acetylation (H4K12ac) in common bean (Phaseolus vulgaris L.) under rust (Uromyces appendiculatus) stress using two high-throughput approaches, chromatin immunoprecipitation sequencing (ChIP-Seq) and RNA sequencing (RNA-Seq). ChIP-Seq analysis revealed 1,235 and 556 histone methylation and acetylation responsive genes from common bean leaves treated with the rust pathogen at 0, 12 and 84 hour-after-inoculation (hai), while …