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Full-Text Articles in Medicine and Health Sciences
Role Of Lipolysis And Lipogenesis In The Development Of Diet-Induced Obesity, Michael John Davies
Role Of Lipolysis And Lipogenesis In The Development Of Diet-Induced Obesity, Michael John Davies
Theses and Dissertations in Biomedical Sciences
Obesity is an increasingly common public health problem with approximately one-half of the American adult population overweight and one-quarter considered obese. This alarming trend has led researchers to determine potential causative factors of excess weight gain in humans. However, it is difficult to discern whether perturbations that result in obesity are the cause or simply the result of the obese state. Diet-induced obesity is one of the animal models that allow researchers to address temporal issues. Our laboratory utilizes a diet-induced obesity model in which Sprague-Dawley rats are placed on a purified moderately high fat diet and ultimately diverge into …
Characterization Of Human T-Cell Leukemia Virus 1 Reverse Transcriptase, Pinky Gundayao Agbuya
Characterization Of Human T-Cell Leukemia Virus 1 Reverse Transcriptase, Pinky Gundayao Agbuya
Theses and Dissertations in Biomedical Sciences
Human T-cell leukemia virus 1 (HTLV-1) is a type C human retrovirus which has been the causative agent of Adult T-cell leukemia. Replication of the retrovirus; requires a reverse transcriptase which converts the retroviral RNA into DNA which is later incorporated into the host's genome. Very little is known about the reverse transcriptase of HTLV-1. Researchers have attempted to purify HTLV-1 RT by isolating the enzyme from human cell lines. Because large amounts of protein could not be produced by this isolation method, the reverse transcriptase cannot be fully characterized. In this research, a recombinant protein expressed in E. coli …
Telomerase Activity In Human Preimplantation Embryos, Diane Lynne Wright
Telomerase Activity In Human Preimplantation Embryos, Diane Lynne Wright
Theses and Dissertations in Biomedical Sciences
Telomerase, a ribonucleoprotein, has been described as an essential component of highly proliferative cells, which stabilizes the telomeres and avoids cellular senescence. Telomerase has been identified in various embryonic cell stages, hematopoietic cells, and in >85% of tumor tissue biopsies analyzed. The ability to measure the potential to proliferate successfully could provide an objective measure of an embryo's quality. The objectives of this study were to modify the telomeric repeat amplification protocol (TRAP) assay system for increased sensitivity to allow detection of telomerase activity in the single cell of an oocyte and embryo, obtain telomerase activity levels for the oocyte …