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Apigenin Ameliorates Hepatic Lipid Accumulation By Activating The Autophagy-Mitochondria Pathway, Man Chen Hsu, Bei Chia Guo, Po-An Hu, Chia Hui Chen, Tzong-Shyuan Lee

Journal of Food and Drug Analysis

Apigenin, a flavonoid isolated from plants, provides protection against non-alcoholic fatty liver disease. However, the mechanism by which apigenin decreases lipid accumulation in the liver is unclear. In this study, we investigated the molecular mechanism underlying the beneficial effect of apigenin on the hepatic deregulation of lipid metabolism. Oleic acid (OA)-induced lipid accumulation in human hepatoma cells (Huh7 cells) was used as an in vitro model. Western blot analysis was used for evaluating protein expression. Oil red O staining, Nile red staining, and conventional assay kits were used to assess the level of lipids. Immunocytochemistry was performed to observe mitochondrial …