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Full-Text Articles in Medicine and Health Sciences
Comparison Of Six Commercial Elisa Kits For Their Specificity And Sensitivity In Detecting Different Major Peanut Allergens, Shyamali Jayasena, Mieke Smits, Daniëlle Fiechter, Aard De Jong, Julie A. Nordlee, Joe Baumert, Steve L. Taylor, Raymond H. Pieters, Stef J. Koppelman
Comparison Of Six Commercial Elisa Kits For Their Specificity And Sensitivity In Detecting Different Major Peanut Allergens, Shyamali Jayasena, Mieke Smits, Daniëlle Fiechter, Aard De Jong, Julie A. Nordlee, Joe Baumert, Steve L. Taylor, Raymond H. Pieters, Stef J. Koppelman
Department of Food Science and Technology: Faculty Publications
Six commercial peanut enzyme-linked immunosorbent assay kits were assessed for their ability to recover peanut from the standard reference material 2387 peanut butter and also for their specificity in detecting four major peanut allergens, Ara h 1, Ara h 2, Ara h 3, and Ara h 6. The percentage recovery of peanut from peanut butter differed across different kits as well as at different sample concentrations. The highest recovery was observed with the Romer and R-Biopharm kits, while four other kits were found to underestimate the protein content of the reference peanut butter samples. Five of the kits were most …