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Expression Of The \Beta-(1,3-1,4)-Glucanase Gene In Streptococcus Salivarius Subsp. Thermophilus, Meltem Aşan, Numan Özcan
Expression Of The \Beta-(1,3-1,4)-Glucanase Gene In Streptococcus Salivarius Subsp. Thermophilus, Meltem Aşan, Numan Özcan
Turkish Journal of Veterinary & Animal Sciences
The purpose of this study was the transformation and expression of the \beta -(1,3-1,4)-glucanase (lichenase) gene in Streptococcus salivarius subsp. thermophilus to create a recombinant probiotic for poultry and improve the thermostability of the lichenase enzyme. The recombinant plasmid TL1R containing the \beta -(1,3-1,4)-glucanase gene was introduced into S. salivarius subsp. thermophilus by electrotransformation. The expressing of the \beta -(1,3-1,4)-glucanase gene in S. salivarius subsp. thermophilus was confirmed on lichenan plate, SDS-PAGE, and zymogram analysis. The \beta -(1,3-1,4)-glucanase enzyme expressed by S. salivarius subsp. thermophilus cells seemed to increase its capacity for thermoresistance and so it maintained its activity at …