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Phosphorylation Of Gap-43 And The Effect On App Processing, Kaitlynn Tracy, Eric Norstrom

DePaul Discoveries

The phosphorylation of Growth Associated Protein may affect the rate of interaction between GAP-43 and the Amyloid Precursor Protein. By synthesizing mutants of the GAP-43 protein, the amount of C-terminal fragments of APP that results from the interaction could be observed under various conditions. The two mutants, S41A and S41D, and the wild type GAP-43 were both co-expressed with APP in moues neuroblastoma cells and analyzed on a Western blot. The mutant used to block phosphorylation showed a greater rate of interaction with APP, while the mutant that was created to mimic the effect of phosphorylation showed less interaction. Further …