Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Medicine and Health Sciences
Measurements Of Phospholipases A2, C And D (Pla2, Plc And Pld) : In Vitro Microassays, Analysis Of Enzyme Isoforms, And Intact-Cell Assays, Ramadan I. Sha’Afi
Measurements Of Phospholipases A2, C And D (Pla2, Plc And Pld) : In Vitro Microassays, Analysis Of Enzyme Isoforms, And Intact-Cell Assays, Ramadan I. Sha’Afi
UCHC Articles - Research
Phospholipases have been studied in great detail and their role in cell signaling has been established in the last 20 years. A role for phospholipases in mitogenesis has been described in terms of the products of their enzymatic reactions. Lysophosphatidic acid (LPA) is produced by the action of a phosphatidic acid (PA)-specific phospholipase A2 (PLA2). LPA is a potent mitogen for quiescent fibroblasts and triggers GTP-dependent phosphoinositide breakdown (1) through a membrane receptor (2). A phosphatidylcholine-specific PLA2 is responsible for the release of arachidonic acid in response to cell stimulation. Arachidonic acid is the precursor of biologically-active eicosanoids such as …