Medicine and Health Sciences Commons^™

Increased Body Weight In Adulthood Following A Peripubertal Stressor And Proposed Mechanism For Effects Of Increased Adiposity On Estrogen-Dependent Behaviors, Christina F. Gagliardi

Masters Theses

Exposure to certain stressors during a sensitive period around puberty can lead to enduring effects on an animal’s response to estradiol. In estradiol-influenced behaviors, such as sexual receptivity, hippocampal-dependent learning and memory, depression-like behavior, and anxiety-like behaviors, exposure to a peripubertal stressor such as shipping stress or an injection of lipopolysaccharide (LPS) can eliminate or even reverse the normal response to estradiol. In addition to regulating these behaviors, estradiol play a role in the regulation of body weight. While some of the previous studies touched on short-term effects on body weight, no systemic long-term study of the effects of a …

The Role Of Sleep Timing On Diet Quality And Physical Activity Among College-Aged Women Participating In The University Of Massachusetts Vitamin D Status Study, Deniz Azarmanesh

Masters Theses

Overweight and obesity are risk factors for various chronic diseases. Sleep has been associated with overweight and obesity. One potential mechanism by which sleep may lead to overweight and obesity is through positive energy balance (i.e. energy intake exceeding expenditure). There are few studies examining the impact of sleep timing (onset and wake time) on diet and exercise. In a cross sectional study, we examined associations between sleep timing and diet quality, and in separate models, between sleep timing and meeting the 2008 Physical Activity Guidelines for Americans among the participants of the UMass Vitamin D Status Study. A total …

Kinetic Asymmetries During Submaximal And Maximal Speed Running, Devon H. Frayne

Masters Theses

An important issue for sports scientists, coaches and athletes is an understanding of the factors within a running stride that can enhance or limit maximal running speed. Previous research has identified many sprint-related parameters as potential kinetic limiters of maximal Center of Mass velocity (Chapman and Caldwell, 1983b; Weyand et al., 2001). Bilateral asymmetry is present for many of these parameters during running; however the degree to which such asymmetries change as running speed increases is unknown. It was hypothesized that asymmetries in key sprinting parameters would be larger at maximal speed than all other tested speeds. Kinematics and kinetics …

Rapid Method Of Processing Sperm For Nucleic Acid Extraction In Clinical Research, Matthew K. De Gannes

Masters Theses

Background: Sperm contain highly compact nuclei, inhibiting DNA extraction using traditional techniques. Current methods extracting sperm DNA involve lengthy lysis and no means of stabilizing DNA, hindering clinical research.

Objective: We sought to optimize an efficient method of extracting high quality human sperm DNA.

Methods: Sperm from three volunteers were isolated using PureCeption. We tested 1) proteinase K with DNA/RNA Shield, 2) DTT and TCEP as reducing agents, 3) QIAshredder homogenization, and 4) stability of sperm DNA fresh (baseline) or after 4 weeks of storage at 4^OC in DNA/RNA Shield using modified Quick-gDNA MiniPrep. DNA was PCR amplified …

Vaccinia Virus Flll Mediated Expedition Of Tanapoxvirus Replication In Cell Culture, Yih Wen Goh

Masters Theses

Tanapoxvirus (TPV) produces large but slow-forming plaques as opposed to vaccinia virus (VACV) that forms similar large plaques but more rapidly. A number of genes were identified in VACV, inclucding FllL, A33R, A34R and A36R that contribute to the regulation of virus release and dissemination, and are particularly responsible for the induction of actin tails. Among them, TPV lacks homologs of the FllL and A36R genes. F11Lmediated inhibition of RhoA-mDia signaling was shown to enhance the microtubules dynamics and modulates the cortical actin that assisted in the release of progeny virus from infected cells. To understand the possible effects of …