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Loma Linda University Electronic Theses, Dissertations & Projects
Development Gene Transcription; Noncoding DNA; Morphogenesis; CNR-Green flourescent protein; Fluorescent Microscopy Localization;
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Quantitative Characterization Of Conserved Noncoding Regions By Flow Cytometry, Amber Brown
Quantitative Characterization Of Conserved Noncoding Regions By Flow Cytometry, Amber Brown
Loma Linda University Electronic Theses, Dissertations & Projects
Spatial and temporal regulation of developmental gene transcription often involves regulatory sequences found in noncoding DNA separate from the gene’s promoter. Conservation of a noncoding region (CNR) across divergent species may indicate a regulatory region critical to basic morphogenesis. CNR-green fluorescent protein (GFP) constructs can demonstrate spatial and temporal CNR activity by microscopy visualization. However, characterization of regulatory modules within a CNR requires quantitative, in addition to qualitative, analysis. The study presented here describes the development and implementation of a novel application of flow cytometry in the quantitative characterization of CNRs. The technique couples fluorescent microscopy localization with flow cytometry …