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- Keyword
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- Development Gene Transcription; Noncoding DNA; Morphogenesis; CNR-Green flourescent protein; Fluorescent Microscopy Localization; (1)
- Gemcitabine Therapy; Pancreatic Cancer; Chemotherapy; Radiotherapy; Survivin Transcription (1)
- Maternal hypoxia; Perinatal hypoxic-ischemic encephalopathy; Systematic asphyxia; Organogenesis (1)
- Runt-related Transcription Factor 2; Thyroid Stimulating Hormone; Thyroid Response Element; Endochondral Ossification (1)
- Survivin-T34A; Pancreatic Cancer; Cancer Fatality; Anti-metabolity Agent; Gemcitabine; Genotoxic Stress (1)
Articles 1 - 8 of 8
Full-Text Articles in Medicine and Health Sciences
Modulation Of T Lymphocytes By Tumor-Released Survivin, Jessica Marie Jutzy
Modulation Of T Lymphocytes By Tumor-Released Survivin, Jessica Marie Jutzy
Loma Linda University Electronic Theses, Dissertations & Projects
The tumor microenvironment is an area of intense interaction between normal and malignant cells. Factors and cell types within this environment can play a crucial role in the progression or regression of the tumor. Of primary interest are tumor-infiltrating T lymphocytes, which have been shown to have a key role in modifying the dynamics of the tumor microenvironment to promote or prevent tumor growth. While there is much in vitro and in vivo evidence for a modification of the tumor infiltrating T cell population toward a pro-tumor environment, what induces these changes within the tumor microenvironment has remained elusive. Our …
Cd21 And Cd24 Co-Expression: A Translational Model Between Mouse And Human, Abigail Benitez
Cd21 And Cd24 Co-Expression: A Translational Model Between Mouse And Human, Abigail Benitez
Loma Linda University Electronic Theses, Dissertations & Projects
Systemic Lupus Erythematosus and Rheumatoid Arthritis are B cell-mediated autoimmune diseases that afflict millions of people worldwide. B cell-targeted therapies for these diseases result in variable clinical outcomes. Thus, a need exists to better understand the dynamics of human B cell production and function. The mouse model has provided a foundation for understanding the mechanisms involved in human B cell development and autoimmune disease. However, differences in mouse and human B cells are not fully understood. Our work shows that the co-expression of CD21 and CD24, determined by 7-color flow cytometry, can be used to demarcate developmental subsets of B …
Quantitative Characterization Of Conserved Noncoding Regions By Flow Cytometry, Amber Brown
Quantitative Characterization Of Conserved Noncoding Regions By Flow Cytometry, Amber Brown
Loma Linda University Electronic Theses, Dissertations & Projects
Spatial and temporal regulation of developmental gene transcription often involves regulatory sequences found in noncoding DNA separate from the gene’s promoter. Conservation of a noncoding region (CNR) across divergent species may indicate a regulatory region critical to basic morphogenesis. CNR-green fluorescent protein (GFP) constructs can demonstrate spatial and temporal CNR activity by microscopy visualization. However, characterization of regulatory modules within a CNR requires quantitative, in addition to qualitative, analysis. The study presented here describes the development and implementation of a novel application of flow cytometry in the quantitative characterization of CNRs. The technique couples fluorescent microscopy localization with flow cytometry …
Exosomal Survivin-T34a: A Novel, Potential Cancer Therapeutic, Jonathan Richard Aspe
Exosomal Survivin-T34a: A Novel, Potential Cancer Therapeutic, Jonathan Richard Aspe
Loma Linda University Electronic Theses, Dissertations & Projects
Pancreatic cancer is the fourth most common cause of cancer fatality in American men and women with a less than 5% survival rate. Currently, if diagnosed early, surgical resection remains the only viable cure. However, only 20% of pancreatic cancer patients meet these criteria. It is therefore necessary to discover new therapies or therapeutic combinations in order to impact significantly this deadly disease. The anti-metabolite agent Gemcitabine is currently being employed to treat pancreatic cancer. While Gem-citabine has shown significant benefit in clinical applications, its ability to more than modestly impact pancreatic cancer is limited. It has been speculated that …
Effect Of Maternal Hypoxia On Fetal Development: Programming Of Hie Phenotype In Neonatal Rat Brain, Pablo J. Gonzalez-Rodriguez
Effect Of Maternal Hypoxia On Fetal Development: Programming Of Hie Phenotype In Neonatal Rat Brain, Pablo J. Gonzalez-Rodriguez
Loma Linda University Electronic Theses, Dissertations & Projects
Developmental programming of health and disease correlates the adverse intrauterine environment and the risk of disease later in life as cardiovascular disease, metabolic diseases, renal dysfunction or neurobehavioral disorders among others. Maternal hypoxia is one of the most common intrauterine growth restricted environment during pregnancy. Perinatal hypoxic-ischemic encephalopathy is an important cause of brain injury in the newborn due to a systematic asphyxia. Our study aims to test the hypothesis that maternal hypoxia affect the organogenesis of the kidney by affecting the kidney structure and focusing on the roles of AT1R and AT2R, while the brain development will also be …
Benefits And Barriers To Hiv Testing In A Population Of Federal Detention Inmates, Nicole A. Knight
Benefits And Barriers To Hiv Testing In A Population Of Federal Detention Inmates, Nicole A. Knight
Loma Linda University Electronic Theses, Dissertations & Projects
Voluntary HIV testing in combination with early medical intervention has been shown to be effective in reducing the rate of new infections and improving the health and life expectancy of those living with HIV. There are however several high-risk populations in the US that remain largely unreached by HIV testing efforts, some of which could be reached while they spend time in prison or jail. Per current Bureau of Prisons (BOP) policy, HIV testing is routinely offered only to “high risk” pretrial and sentenced inmates. We conducted a cross-sectional study with 721 pretrial and sentenced inmates who entered a large …
Retinoic Acid Regulation Of Thyroid Hormone Action In Bone Cells, Anjali Babbar
Retinoic Acid Regulation Of Thyroid Hormone Action In Bone Cells, Anjali Babbar
Loma Linda University Electronic Theses, Dissertations & Projects
Retinoic acid and thyroid hormone are known to play key roles in the regulation of endochondral ossification. However, the issue of whether these two hormones interact with each other to regulate bone functions remains to be established. We investigated how thyroid hormone and retinoic acid interact to regulate cells involved in endochondral bone formation. We demonstrate that thyroid hormone treatment stimulates differentiation of ATDC5 chondrocytes and promotes formation of mineralized nodules while retinoic acid treatment at high dose inhibits chondrocyte differentiation and formation of mineralized nodules. Furthermore, thyroid hormone induced mineralized nodule formation is inhibited by co-treatment with retinoic acid …
Survivin: Regulation By Yy1 And Role In Pancreatic Cancer Combination Therapy, Nicholas R. Galloway
Survivin: Regulation By Yy1 And Role In Pancreatic Cancer Combination Therapy, Nicholas R. Galloway
Loma Linda University Electronic Theses, Dissertations & Projects
Despite significant clinical and basic science advancements, cancer remains a devastating disease that affects people of all ages, races, and background. Survivin, the fourth most common transcript found in cancer cells, is a protein that is thought to be involved in the enhanced proliferation, survival, and metastasis of cancer cells. Therefore understanding how this gene is regulated is potentially of vital importance to improving cancer management and therapy. Our work has identified a novel transcriptional regulator of survivin called Yin Yang 1 (YY1). YY1 is a transcription factor that has been observed to activate some gene promoters and repress others, …