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Full-Text Articles in Medicine and Health Sciences
Lncrna Ak001796 Is Essential For The Survival Of Aggressive Breast Cancer Cells, Sumayya Adib Al-Chalabi
Lncrna Ak001796 Is Essential For The Survival Of Aggressive Breast Cancer Cells, Sumayya Adib Al-Chalabi
Legacy Theses & Dissertations (2009 - 2024)
Abstract:
A Breast Cancer Stem Cell Model Created From Mmtv-Pymt Mice Applicable To Human Breast Cancer, Denise Grant Lanza
A Breast Cancer Stem Cell Model Created From Mmtv-Pymt Mice Applicable To Human Breast Cancer, Denise Grant Lanza
Legacy Theses & Dissertations (2009 - 2024)
Cancer stem cells are the seeds of tumor growth, but there are limited cell-based methods that exist to study the properties of these cells. To create a model of breast cancer stem cells, we isolated tumors from MMTV-PyMT mice. Two out of the four different cell types isolated survived in culture, CD44+CD24- and CD24+CD49f+CD44low. We found that we could initiate tumors with as few as 10 cells injected subcutaneously in the hind leg or orthotopically in the cleared fat pad with CD24+ cells. However, we could not initiate tumors with injection of CD24- cells. We found a requirement for TICs …