Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Medicine and Health Sciences
Steps In Metagenomics: Let’S Avoid Garbage In And Garbage Out, Jacques Izard
Steps In Metagenomics: Let’S Avoid Garbage In And Garbage Out, Jacques Izard
Department of Food Science and Technology: Faculty Publications
Is metagenomics a revolution or a new fad? Metagenomics is tightly associated with the availability of next-generation sequencing in all its implementations. The key feature of these new technologies, moving beyond the Sanger-based DNA sequencing approach, is the depth of nucleotide sequencing per sample.1 Knowing much more about a sample changes the traditional paradigms of “What is the most abundant?” or “What is the most significant?” to “What is present and potentially significant that might influence the situation and outcome?” Let’s take the case of identifying proper biomarkers of disease state in the context of chronic disease prevention. Prevention …
Comparison Of Six Commercial Elisa Kits For Their Specificity And Sensitivity In Detecting Different Major Peanut Allergens, Shyamali Jayasena, Mieke Smits, Daniëlle Fiechter, Aard De Jong, Julie Nordlee, Joe Baumert, Steve L. Taylor, Raymond H. Pieters, Stef J. Koppelman
Comparison Of Six Commercial Elisa Kits For Their Specificity And Sensitivity In Detecting Different Major Peanut Allergens, Shyamali Jayasena, Mieke Smits, Daniëlle Fiechter, Aard De Jong, Julie Nordlee, Joe Baumert, Steve L. Taylor, Raymond H. Pieters, Stef J. Koppelman
Department of Food Science and Technology: Faculty Publications
Six commercial peanut enzyme-linked immunosorbent assay kits were assessed for their ability to recover peanut from the standard reference material 2387 peanut butter and also for their specificity in detecting four major peanut allergens, Ara h 1, Ara h 2, Ara h 3, and Ara h 6. The percentage recovery of peanut from peanut butter differed across different kits as well as at different sample concentrations. The highest recovery was observed with the Romer and R-Biopharm kits, while four other kits were found to underestimate the protein content of the reference peanut butter samples. Five of the kits were most …