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A Monomeric Protein In The Golgi Membrane Catalyzes Both N-Deacetylation And N-Sulfation Of Heparan Sulfate, Elisabet Mandon, Ellis Kempner, Masayuki Ishihara, Carlos Hirschberg
A Monomeric Protein In The Golgi Membrane Catalyzes Both N-Deacetylation And N-Sulfation Of Heparan Sulfate, Elisabet Mandon, Ellis Kempner, Masayuki Ishihara, Carlos Hirschberg
Elisabet Mandon
Recent studies have shown that the rat liver heparan sulfate N-deacetylase/N-sulfotransferase is a glycoprotein encoded by a single polypeptide chain of 882 amino acids. Using radiation inactivation analyses, we have now determined that in rat liver Golgi vesicles the target size for the N-deacetylase is 88 +/- 14 kDa, whereas that of the N-sulfotransferase is 92 +/- 8 kDa. These results, together with previous biochemical and molecular cloning approaches, demonstrate that 1) in rat liver Golgi membranes there exists only on population of molecules expressing both activities, 2) the active protein in the Golgi membrane functions as a monomer, and …
A Mutant Yeast Deficient In Golgi Transport Of Uridine Diphosphate N-Acetylglucosamine, Claudia Abeijon, Elisabet Mandon, Phillips Robbins, Carlos Hirschberg
A Mutant Yeast Deficient In Golgi Transport Of Uridine Diphosphate N-Acetylglucosamine, Claudia Abeijon, Elisabet Mandon, Phillips Robbins, Carlos Hirschberg
Elisabet Mandon
Mannan chains of Kluyveromyces lactis mannoproteins are similar to those of Saccharomyces cerevisiae except that they have terminal alpha1-->2-linked N-acetylglucosamine and lack mannose phosphate. In a previous study, Douglas and Ballou (Douglas, R. K., and Ballou, C. E. (1982) Biochemistry 21, 1561-1570) characterized a mutant, mnn2-2, which lacked terminal N-acetylglucosamine in its mannoproteins. The mutant had normal levels of N-acetylglucosaminyltransferase activity, and the partially purified enzyme from wild-type and mutant cells had the same apparent size, heat stability, affinity for substrates, metal requirement, and subcellular location. No qualitative or quantitative differences were found between mutant and wild-type cells in …