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Full-Text Articles in Medicine and Health Sciences
Clinically Relevant 3d Characterization Of Simulated Non-Carious Cervical Dental Lesions And The Effects Of Toothbrush And Toothpaste Types On Wear, James Needy
Biomedical Engineering Undergraduate Honors Theses
This study sought to develop a novel method to quantitatively characterize non-carious cervical lesions (NCCLs) using an intraoral scanner (IOS) paired with Geomagic Wrap mesh software. The goal was to compare results with those obtained using an optical profilometer and Proscan superimposition software for clinical application. Comparisons were made using a previously published dataset aimed at measuring tooth loss associated with differing toothbrush and toothpaste types. Results show good comparability between methods and confirm that variation in brush stiffness and paste abrasivity result in significantly and predictably different levels of NCCL development. Extracted human maxillary premolars were assigned to one …
Detection Of Leukocytes Stained With Acridine Orange Using Unique Spectral Features Acquired From An Image-Based Spectrometer, Courtney J. Hunter
Detection Of Leukocytes Stained With Acridine Orange Using Unique Spectral Features Acquired From An Image-Based Spectrometer, Courtney J. Hunter
Biomedical Engineering Undergraduate Honors Theses
A leukocyte differential count can be used to diagnosis a myriad blood disorders, such as infections, allergies, and efficacy of disease treatments. In recent years, attention has been focused on developing point-of-care (POC) systems to provide this test in global health settings. Acridine orange (AO) is an amphipathic, vital dye that intercalates leukocyte nucleic acids and acidic vesicles. It has been utilized by POC systems to identify the three main leukocyte subtypes: granulocytes, monocytes, and lymphocytes. Subtypes of leukocytes can be characterized using a fluorescence microscope, where the AO has a 450 nm excitation wavelength and has two peak emission …
Optical Imaging Of Metabolic Adaptability As A Biomarker For Metastatic Potential In Breast Cancer Cells, Mason G. Harper
Optical Imaging Of Metabolic Adaptability As A Biomarker For Metastatic Potential In Breast Cancer Cells, Mason G. Harper
Biomedical Engineering Undergraduate Honors Theses
Breast cancer metastasis is the main cause for mortality in breast cancer patients. However, knowledge of metastatic recurrence is limited, and there is a need to understand metastatic recurrence in order to treat breast cancer patients more effectively. Highly invasive metastatic breast cancer has shown to exhibit metabolic adaptability, transitioning from glycolysis to oxidative phosphorylation in the presence of microenvironmental stress. NADH and FAD are naturally occurring cofactor products during glycolysis and oxidative phosphorylation, respectively, and they are of particular importance during these metabolic processes due to their endogenous fluorescence. Measuring the ratio of fluorescence intensities of these cofactors through …
Microdialysis Studies Using Porcine Pancreatic Elastase To Guide Mathematical Modeling Of Microdialysis Sampling For In Vivo Measurements., Mason W. Young
Microdialysis Studies Using Porcine Pancreatic Elastase To Guide Mathematical Modeling Of Microdialysis Sampling For In Vivo Measurements., Mason W. Young
Biomedical Engineering Undergraduate Honors Theses
Microdialysis sampling uses a semi-permeable membrane to allow solute collection by diffusion. When used in conjunction with other instruments, analytes in question can be quantified. Matrix Metalloproteinases (MMPs) are enzymes involved in numerous biological processes where they serve the role of degrading extracellular matrix. Microdialysis sampling, in coordination with further analysis methods, can be used in order to measure the activity of these enzymes in a region of the body instantaneously. The intention of this project is to determine ways of measuring in vitro activity of elastase from porcine pancreas using determined activity values and the collection of elastase products. …