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Full-Text Articles in Medicine and Health Sciences
Toward The Synthesis Of Nuclease Models., Enni Nina Fomumbod
Toward The Synthesis Of Nuclease Models., Enni Nina Fomumbod
Electronic Theses and Dissertations
Nucleases are enzymes that can specifically recognize nucleic acids and hydrolyze their phosphodiester bonds effectively. As is the case with many hydrolases, nucleases often carry one or more metal centers. Cooperation between such metal centers and other interactions involving general acid-base activities are believed to be essential in multifunctional catalyses. Combination of such interactions in model compounds often resulted in larger than additive effects.
This work is aimed at synthesizing nuclease models that combine the ability to recognize phosphate groups and/or nitrogen bases of DNA together with the ability to catalyze phosphodiester hydrolysis. These models were designed to achieve optimum …
Expression, Purification, And Characterization Of The Mast Cell Proteases Chymase And Cathepsin G., Brent E. Lockhart
Expression, Purification, And Characterization Of The Mast Cell Proteases Chymase And Cathepsin G., Brent E. Lockhart
Electronic Theses and Dissertations
Human mast cells have been associated with wound healing, allergies, inflammation, and defense against pathogens and have been detected in tissues close to blood vessels especially in the areas between the inside of the body and the external environment, such as the skin, lungs, digestive tract, mouth, and nose. Previous studies have shown that mast cells contain large granules filled with histamine, heparin, cytokines, eicosanoids, and the serine proteases, tryptase, Chymase, and cathepsin G (CatG). These proteases are stored and released from mast-cell granules upon activation by antigen binding to IgE immunoglobulins on the cell surface or by direct injury. …