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Full-Text Articles in Medicine and Health Sciences
Metal-Resistance Genetically Engineered Bacteria, Sylvia Daunert, Donna Scott, Sridhar Ramanathan
Metal-Resistance Genetically Engineered Bacteria, Sylvia Daunert, Donna Scott, Sridhar Ramanathan
KWRRI Research Reports
Bacterial-based electrochemical and optical sensing systems that respond in a highly selective and sensitive manner to antimonite and arsenite have been developed. This was accomplished by using genetically engineered bacteria bearing one of two plasmids constructed for our studies. The first plasmid, pBGD23, contains the operator/promoter region (O/P) and the gene of the ArsR protein from the ars operon upstream from the β-galactosidase gene. In the absence of antimonite/arsenite, ArsR binds to the 0/P site and prevents the transcription of the genes for ArsR and β-galactosidase, thus blocking expression of these proteins. When antimonite or arsenite is present in the …
The Aquatic Toxicity Of Organic Compounds To Embryo-Larval Stages Of Fish And Amphibians, Jeffrey A. Black, Wesley J. Birge, Willaim E. Mcdonnell, Albert G. Westerman, Barbara A. Ramey, Donald M. Bruser
The Aquatic Toxicity Of Organic Compounds To Embryo-Larval Stages Of Fish And Amphibians, Jeffrey A. Black, Wesley J. Birge, Willaim E. Mcdonnell, Albert G. Westerman, Barbara A. Ramey, Donald M. Bruser
KWRRI Research Reports
Aquatic toxicity tests were conducted on 11 organic compounds considered hazardous to water resources. The toxicity of each compound was evaluated using embryo-larval stages of two to eight fish and amphibian species. Exposure was initiated at fertilization and maintained through 4 days posthatching. The animal test species exhibited varying degrees of sensitivity to the selected toxicants. Combined frequencies for mortality and teratogenesis at 4 days posthatching gave LC50 ranges of 3.66 to 8.25 mg/L for benzene, 1.16 to 22.42 mg/L for carbon tetrachloride, 0.11 to 1.20 mg/L for chlorobenzene, 2.03 to > 68 mg/L for chloroform, 3.01 to 5.56 mg/L …