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Full-Text Articles in Medicine and Health Sciences
Enhanced Detection Of Surface-Associated Bacteria In Indoor Environments By Quantitative Pcr, Mark P. Buttner, Patrica Cruz-Perez, Linda Stetzenbach
Enhanced Detection Of Surface-Associated Bacteria In Indoor Environments By Quantitative Pcr, Mark P. Buttner, Patrica Cruz-Perez, Linda Stetzenbach
Environmental & Occupational Health Faculty Publications
Methods for detecting microorganisms on surfaces are needed to locate biocontamination sources and to relate surface and airborne concentrations. Research was conducted in an experimental room to evaluate surface sampling methods and quantitative PCR (QPCR) for enhanced detection of a target biocontaminant present on flooring materials. QPCR and culture analyses were used to quantitateBacillus subtilis (Bacillus globigii) endospores on vinyl tile, commercial carpet, and new and soiled residential carpet with samples obtained by four surface sampling methods: a swab kit, a sponge swipe, a cotton swab, and a bulk method. The initial data showed that greater overall sensitivity was obtained …