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Full-Text Articles in Medicine and Health Sciences

Novel Application Of Graphene Oxide Based-Biosensor Utilizing Crispr/Dcas9 For Rapid Detection Of Antibiotic Resistance Genes, Joel Omage Oct 2020

Novel Application Of Graphene Oxide Based-Biosensor Utilizing Crispr/Dcas9 For Rapid Detection Of Antibiotic Resistance Genes, Joel Omage

Masters Theses & Specialist Projects

Antibiotic resistance is one of the biggest public health challenges globally. To combat this threat, we have designed a method for the facile and rapid detection of antibiotic resistance gene (ARG) in bacteria. We utilized an emerging tool termed the clusters of regularly interspaced short palindromic repeats (CRISPR) in complex with deactivated CRISPR associated proteins (dCas9) on conjugated 2-dimensional graphene oxide (GO) sheets. Nuclease-deactivated Cas9 in complex with customized single guided RNA (sgRNA) was used for the target DNA recognition. The sgRNA was easy to design and we demonstrated that it is complementary to its target sequence by performing a …


Team Creation Methods In Practice: Understanding The Potential Effects Of Nonverbal Communication In The Leadership Of Team Formation, Troy Allen Robertson Apr 2020

Team Creation Methods In Practice: Understanding The Potential Effects Of Nonverbal Communication In The Leadership Of Team Formation, Troy Allen Robertson

Dissertations

Teams require leadership, even if they are self-managed. The group of individuals who make up a team must be gathered in some form or another. For self managed teams to function successfully, the first step is the process of creating the team. Many aspects may factor into the creation process. Often time is of the essence and methods to quickly assess and form teams show merit. First impressions in general are based largely on nonverbal communication. The focus of this mixed-methods concurrent embedded study is to analyze the potential effects of nonverbal communication on influencing team creation. A group of …


Learning To Jump Like Corbin Bleu: The Physics Behind Jump Rope, Caroline Camfield Jan 2020

Learning To Jump Like Corbin Bleu: The Physics Behind Jump Rope, Caroline Camfield

Mahurin Honors College Capstone Experience/Thesis Projects

This project seeks to determine how engineering can be applied to the sport of jump rope so that athletes may better understand the science behind the sport and apply it to improve their performance in “speed jumping.” This style of jump rope consists of a jumper alternating their feet, with the rope passing under them with each step. Several types of ropes, handles, rope lengths, and widths between a jumper’s handles were tested to determine correlations between each variable and different STEM concept. These findings were then transitioned into a lesson plan so that both the jumpers and coaches could …