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Full-Text Articles in Medicine and Health Sciences
Analysis Of Circulating Tumour Cells In Early-Stage Uveal Melanoma: Evaluation Of Tumour Marker Expression To Increase Capture, Aaron B. Beasley, Timothy W. Isaacs, Tersia Vermeulen, James Freeman, Jean-Louis De Sousa, Riyaz Bhikoo, Doireann Hennessy, Anna Reid, Fred K. Chen, Jacqueline Bentel, Daniel Mckay, R. Max Conway, Michelle R. Pereira, Bob Mirzai, Leslie Calapre, Wendy N. Erber, Melanie R. Ziman, Elin S. Gray
Analysis Of Circulating Tumour Cells In Early-Stage Uveal Melanoma: Evaluation Of Tumour Marker Expression To Increase Capture, Aaron B. Beasley, Timothy W. Isaacs, Tersia Vermeulen, James Freeman, Jean-Louis De Sousa, Riyaz Bhikoo, Doireann Hennessy, Anna Reid, Fred K. Chen, Jacqueline Bentel, Daniel Mckay, R. Max Conway, Michelle R. Pereira, Bob Mirzai, Leslie Calapre, Wendy N. Erber, Melanie R. Ziman, Elin S. Gray
Research outputs 2014 to 2021
Background:
The stratification of uveal melanoma (UM) patients into prognostic groups is critical for patient management and for directing patients towards clinical trials. Current classification is based on clinicopathological and molecular features of the tumour. Analysis of circulating tumour cells (CTCs) has been proposed as a tool to avoid invasive biopsy of the primary tumour. However, the clinical utility of such liquid biopsy depends on the detection rate of CTCs.
Methods:
The expression of melanoma, melanocyte, and stem cell markers was tested in a primary tissue microarray (TMA) and UM cell lines. Markers found to be highly expressed in primary …
Multi-Marker Immunofluorescent Staining And Pd-L1 Detection On Circulating Tumour Cells From Ovarian Cancer Patients, Du-Bois Asante, Michael Morici, Ganendra R. K. A. Mohan, Emmanuel Acheampong, Isaac Spencer, Weitao Lin, Paula Van Miert, Samantha Gibson, Aaron B. Beasley, Melanie Ziman, Leslie Calapre, Tarek M. Meniawy, Elin S. Gray
Multi-Marker Immunofluorescent Staining And Pd-L1 Detection On Circulating Tumour Cells From Ovarian Cancer Patients, Du-Bois Asante, Michael Morici, Ganendra R. K. A. Mohan, Emmanuel Acheampong, Isaac Spencer, Weitao Lin, Paula Van Miert, Samantha Gibson, Aaron B. Beasley, Melanie Ziman, Leslie Calapre, Tarek M. Meniawy, Elin S. Gray
Research outputs 2014 to 2021
Detection of ovarian cancer (OC) circulating tumour cells (CTCs) is primarily based on targeting epithelial markers, thus failing to detect mesenchymal tumour cells. More importantly, the immune checkpoint inhibitor marker PD-L1 has not been demonstrated on CTCs from OC patients. An antibody staining protocol was developed and tested using SKOV-3 and OVCA432 OC cell lines. We targeted epithelial (cytokeratin (CK) and EpCAM), mesenchymal (vimentin), and OC-specific (PAX8) markers for detection of CTCs, and CD45/16 and CD31 were used for the exclusion of white blood and vascular endothelial cells, respectively. PD-L1 was used for CTC characterisation. CTCs were enriched using the …