Medicine and Health Sciences Commons^™

Squamous Cell Carcinoma Response To Cisplatin Exposure, Khanh V. Luong

Theses & Dissertations

Purpose: This thesis attempted to quantitatively analyze the individual cell fate choice in resistant head and neck UM-SCC38 cells exposed to cisplatin using the most current techniques available.

Methods: UM-SCC-38 cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS). They were treated with cisplatin and ATM/ATR inhibitors of known dosages. Using live cell imaging, one hundred cells were tracked in each experiment and their behaviors were analyzed and entered into Microsoft Excel Spreadsheet to generate cell profile graphs. HaCaT cells, non-tumorigenic keratinocyte cell line, were also analyzed using live cell imaging and their …

Quality Of Life And Subjective Outcomes Following Maxillomandibular Advancement Surgery For The Treatment Of Obstructive Sleep Apnea, Andrew D. Emanuele

Electronic Thesis and Dissertation Repository

Objective: The aim of this study was to assess outcomes related to general quality of life, daytime sleepiness and functional sleep outcomes, functional outcomes of orthognathic surgery, and facial aesthetics in patients undergoing maxillomandibular advancement (MMA) surgery for the treatment of obstructive sleep apnea (OSA).

Materials and Methods: This was a cross-sectional self-report study. A questionnaire was constructed using questions drawn from previously validated questionnaires. The survey was distributed to 25 patients who underwent MMA surgery for the treatment of OSA at LHSC in London, Ontario by a single surgeon between 2002 and 2013.

Results: The survey results showed that …

The Role Of Bone Sialoprotein In Periodontal Tissue Development And Bone Repair, Yohannes Soenjaya

Electronic Thesis and Dissertation Repository

Bone development and repair involve complex processes that include interaction between cells and their surrounding matrix. In the body, bone sialoprotein (BSP) expression is up-regulated at the onset of mineralization. BSP is a multifunctional acidic phosphoprotein with collagen-binding, hydroxyapatite nucleating, and integrin recognition (RGD sequence, which is important for cell-attachment and signaling) regions. Mice lacking BSP expression (Bsp^-/-), exhibit a bone phenotype with reductions in bone mineral density, bone length, osteoclast activation, and impaired bone healing. This thesis examined the role of BSP in tooth development and also its potential use as a therapeutic reagent for bone …

Dental Hygienists' Cognitive Process In Periodontal Soft Tissue Charting, Kelsey M. Schwei

Theses and Dissertations

Introduction: Dental hygienists have not had the opportunity to be involved in the design and development of the periodontal soft tissue charts and the surrounding interface features that are used while examining dental patients in daily practice. In some cases, dentists are able to give their opinions, wants, and needs into the development of the health information systems that they use on a daily basis, but too often, the dental hygienist is forgotten about and no input is ever given to the developers from the dental hygienists. This project considers the impact of well-designed interfaces on effectiveness and workflow particularly …

Assessing Orthodontic-Bracket Impacts On Lip Profile At Bonding And Debonding Stages, Martin Trockel

Student Theses, Dissertations and Capstones

Introduction: One factor that contributes greatly to the lower face appearance and orthodontists have the ability to affect is lip profile. Clinical assessment of the lip profile is an important element in the decision of orthodontic treatment planning and in the evaluation of treatment progress and outcome. Three known factors influence the lip profile; the lip thickness, the underlying bone, and the tooth position. The positions and inclinations of the anterior teeth can affect the lip position, but it is unclear whether orthodontic brackets bonded to the labial surface of anterior teeth move the lip position and thereafter change the …

Effects Of Sodium Hypochlorite On Enamel Composition, Sonni Pellillo

Student Theses, Dissertations and Capstones

The purpose of this study was to evaluate the effects of sodium hypochlorite on the organic and inorganic composition of enamel. Background: With the advent of enamel bonding for orthodontic appliances in the late 1970s, it has been shown that traditional phosphoric acid etching affects the inorganic portion of the enamel.1, 2 In an attempt to enhance the acid etching pattern and, furthermore, the bond strength, additional pretreatment techniques that target the organic components of the enamel biofilm have been proposed. One such method is the non-invasive enamel pretreatment with 5.25% sodium hypochlorite (NaOCl) prior to phosphoric acid etching.3, 4 …

Evaluation Of Frictional Forces Between Brackets Of Different Types At Various Angulations And An Arch Wire: With And Without Pulsating Vibration, Benjamin M. Christman

Student Theses, Dissertations and Capstones

Objective: The objective of this study was to determine the effect of pulsating vibration on the sliding resistance between orthodontic brackets and stainless steel wires. Brackets were placed at two different angulations (0° and 5°) to simulate leveling of a tipped tooth during tooth movement. Pulsating vibration was delivered via the AcceleDent device.

Background: Friction is defined as a force that retards or resists the relative motion of two objects in contact, and its direction is tangential to the common boundary of the two surfaces in contact. This has been of interest to the orthodontist since the mid-20th century. Since …

Comparison Of Digital Scanning And Polyvinyl Siloxane Impression Techniques By Dmd Students : Instructional Efficiency And Attitudes Toward Technology., Alexander M. Marti

Electronic Theses and Dissertations

The aim of this research was compare the ability of dental students to learn an digital scanner (3M LAVA) and material-based (PVS) impression techniques and to determine attitudes and preferences towards each. D2 dental students (n=25) were recruited and instructed on the use of 3M LAVA and PVS techniques using three pedagogic methods: 1) video lecture (P1), 2) investigator-led demonstration (P2), and 3) independent clinical exercise (P3). The amount of time for each pedagogic method was measured, averaged (± s.d.) and compared using Wilcoxon Signed-Ranks tests. A pre- and post-test was administered assessing their attitudes towards both techniques using a …

A 3d Radiographic Evaluation Of Crestal Bone Changes Around Immediately Loaded Endosseous Implants, Keerthi Senthil

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this clinical investigation is to evaluate and compare the 3-dimensional radiographic crestal bone changes around 4 immediately placed and loaded implants supporting full arch fixed mandibular prosthesis in 2 groups of patients. The Test group comprises of patients with failing mandibular teeth needing extraction. ( due to caries, periodontal disease, fractures or prosthetic reasons).The Control group comprises of patients with a fully edentulous mandible presenting for fixed implant supported prosthesis with at least 6 months after the last extraction or bone grafting procedure. Stereolithographic bone model obtained from the patient’s DICOM files was used to fabricate the …

Synthesis And Functional Evaluation Of Peptide Modified Poly (Lactic-Co-Glycolic Acid) Nanoparticles To Inhibit Porphyromonas Gingivalis Biofilm Formation., Paridhi Kalia

Electronic Theses and Dissertations

Periodontal disease is an oral inflammatory disorder that afflicts roughly 46% of the adults in the U.S. Currently, treatment of periodontal disease involves the removal of plaque from the gingival pocket (with possible antibiotic treatment) and if necessary, gingival surgery. To our knowledge, no therapeutic approach exists that promotes host-biofilm homeostasis by limiting pathogen recolonization of the oral cavity after prophylaxis or treatment. The interaction of the pathogen Porphyromonas gingivalis with commensal streptococci is critical for initiation of periodontitis and represents a target for limiting P. gingivalis colonization of the oral cavity. Previous studies showed that a synthetic peptide …

Access To Oral Health For Children On Mainecare In The Lakes Region Towns Of Bridgton, Casco, Sebago, And Naples, Carissa L. Parent

Muskie School Capstones and Dissertations

Although we know that children covered by Medicaid often do not receive needed dental services, the extent of the problem in Maine is not fully understood. In particular, rural communities in Maine may face challenges for families to access dental services for their children given low reimbursement rates and fewer available providers. This project addresses these gaps in knowledge by examining MaineCare- funded utilization and dental care access for children with MaineCare in four rural Maine towns in the Lakes Region.

Cbct Evaluation Of Adolescent Mandibular Morphology In Different Classifications Of Facial Type, Annie Hsu

UNLV Theses, Dissertations, Professional Papers, and Capstones

The goal of this study is to use the improved imaging capability of cone-beam computerized tomography (CBCT) to investigate the relationship between vertical facial patterns and mandibular tooth-alveolar morphology in the adolescent population. Pre-treatment orthodontic records were obtained from the UNLV School of Dental Medicine archival dental records. One hundred and seventy three patients (72 males, 101 females) between the ages of 12 and 18 years were included in this study. Among these patients, 61 displayed the vertical growth pattern, 30 displayed the horizontal growth pattern, and 82 displayed the average growth pattern. The samples were categorized into 4 age …

Scardovia Wiggsiae Prevalence In Orthodontic Patients, Brandon Joseph Streiff

UNLV Theses, Dissertations, Professional Papers, and Capstones

Dental caries has mainly been associated with Streptococcus, Lactobacillus, Actinomyces and Veillonella species. But in recent salivary studies a new cariogenic pathogen, has been identified. This new bacteria, Scardovia wiggsiae (SW), is currently being tested within the UNLV School of Dental Medicine patient population. Although these current studies are being conducted to study its prevalence in both pediatric and adult populations, it has not been evaluated among patients with an altered oral environment as seen in patients with orthodontic appliances. Fixed orthodontic appliances increase the difficulty of removing daily plaque on and in between the teeth with standard oral hygiene …

Assessment Of Orthodontic Patients' Preferences Regarding Treatment From General Dentists Or Orthodontists, Brian Robert Wilde

UNLV Theses, Dissertations, Professional Papers, and Capstones

This study evaluated how parents of prospective orthodontic patients choose between taking their child to a general dentist or an orthodontist for orthodontic treatment. It also looked at which factors influence patients’ satisfaction with the progress of treatment and likelihood to refer other patients to the practice. This study included two phases. The first phase was a qualitative assessment of the decision making process through conducting and analyzing focus groups. The second phase was the development and administration of a nationwide online survey regarding the topics covered in the focus groups. Influential factors in the decision making process fell into …

Dexamethasone Regulates Gene Expression Induced By The Mechanosensitive P2x7 Receptor In Osteoblasts, Meena Na

Electronic Thesis and Dissertation Repository

Orthodontic tooth movement involves activation of osteoblasts and osteoclasts in response to mechanical forces. Extracellular nucleotides, released in response to mechanical stimuli, signal through P2 receptors in osteoblasts. P2X7 receptors are ATP-gated cation channels implicated in mechanotransduction in bone. Corticosteroids such as dexamethasone exhibit anti-inflammatory effects and their chronic use has been linked to serious side-effects such as osteoporosis. We investigated gene expression following P2X7 receptor activation with benzoylbenzoyl-ATP (P2X7 receptor agonist) in osteoblast precursor cells in the presence or absence of dexamethasone. We hypothesized that dexamethasone inhibits the ability of ATP to stimulate the expression of anabolic (bone forming) …

An Evaluation Of The Constructed Frankfort Mandibular Plane Angle Bisector (Cfmab) Wits' Appraisal In The Assessment Of Anteroposterior Jaw Relationships, Derek R. Tomson

Electronic Thesis and Dissertation Repository

For proper orthodontic diagnosis and treatment planning, an accurate measurement of the relationship of the maxilla to the mandible in the sagittal plane is required. The ANB measurement has long been the gold standard for explaining this association. The purpose of this longitudinal study was to evaluate three linear measurements of the maxillomandibular anteroposterior relationship and determine which one best correlates with ANB. The constructed Frankfort horizontal-mandibular plane angle bisector (cFMAB-Wits), maxillomandibular bisector (MMB-Wits), and Frankfort-mandibular plane angle bisector (FMAB-Wits) were measured using a Wits’-type analysis and compared to ANB.

Pre-treatment (TO), immediate post-treatment (T1), and two years post-retention (T2) …

Color Of Porcelain Veneer After Final Cementation In Comparison To Try-In Paste And Permanent Cement: An In Vitro Study, Abdulelah Aldahlawi

Student Theses, Dissertations and Capstones

Objectives: To evaluate and compare the color of porcelain veneers with try-in paste in relation to porcelain veneers with permanent cement. Also, to evaluate and compare combinations of three different shades and thicknesses of porcelain veneers and three cement shades before and after final cementation. Additionally, to evaluate and compare the color of porcelain veneers with cured permanent cement before and after aging. Background: Porcelain veneers and ceramic restorations have become one of the most popular approaches in the anterior area due to their natural appearance and esthetics. However, more conservative approaches have led to thinner restorations with increased translucency. …

Ex Vivo Effects Of An Orthodontic-Specific Bleaching System On Tooth Color And Existing Bracket Bonding, Kirk James Bean

Student Theses, Dissertations and Capstones

Objective: The purpose of this ex vivo study was to assess the changes in tooth color and existing orthodontic bracket bonding when exposed to a bleaching agent. Methods: 120 freshly extracted human premolars were equally divided into 4 groups: a negative-control group, a positive-control bleaching group, a bracket-bonded group, and a bracket bonded-bleached group. The bleaching protocol was carried out as recommended by the manufacturer of Opalescence Go® – 30 minutes each day for 10 consecutive days. The color value of each tooth was measured daily by spectrophotometry for the negative-control group and the positive-control bleaching group in 6 different …

Adjunctive Appliance Wear And Gender Affect Patients' Oral Health-Related Quality Of Life During The Late Stage Of Orthodontic Treatment In Adolescents, Monica Dinh

Student Theses, Dissertations and Capstones

Objective: This study was conducted to determine how oral health-related quality of life (OHQoL) changes during the late stage of comprehensive fixed orthodontic treatment in adolescents and what factors may be involved. Background: Completion of orthodontic treatment has been shown to improve patients’ OHQoL due to the changes in esthetics, function and emotional well-being. The levels of OHQoL fluctuate throughout treatment where an initial decrease within the first 6 months occurs followed by an improvement towards patients’ pre-treatment levels. At 18 months of fixed orthodontic treatment, the level is unclear. The aims of this study were to clarify the changes …

Diabetes Status, Predisposing, Enabling, And Oral Health Illness Level Variables As Predictors Of Preventive And Emergency Dental Service Use, Sabha Alshatrat

Health Services Research Dissertations

The purpose of this study was to clarify the relationship between diabetes and dental service use. This study addressed the question of whether individuals with diabetes are more or less likely to utilize dental services, measured in terms of preventive and emergency services during the past year. A cross-sectional study was conducted to assess the contributions of diabetes status to dental service use, relative to the contributions of Andersen and Newman Framework of Health Services Utilization dimensions (predisposing, enabling, and illness variables) in predicting dental service utilization during the past year using a nationally representative sample from a 2001-2002 National …

Dental Consumers' Perceptions Of Dental Hygienists With Visible Tattoos, Amanda Brooke Verissimo

Dental Hygiene Theses & Dissertations

Purpose: The purpose of this study was to determine if visible tattoos affected the perceptions of dental consumers toward the professionalism and credibility of dental hygienists.

Methods: An 11 item, IRB approved investigator designed survey was administered online to 203 subjects invited to participate via a commercial web source. Surveys were randomized according to respondents' birth month. Participants viewed one of three photographs of a dental hygiene model wearing short-sleeved scrubs without a tattoo, with a large sleeve tattoo on the arm or with a small tattoo on the wrist. Participants scored each photograph on a 5-point Likert scale based …

Mass Spectrometric Analyses Of Surface Proteins From Candida Albicans After Treatment With Histatin 5, Shirley Fan

Electronic Thesis and Dissertation Repository

Current drug therapies have lost their effectiveness in controlling the fungal species known as C. albicans; therefore, the need for newer drugs becomes crucial. Histatin 5 (Hst 5), a naturally occurring peptide found in the oral cavity, has demonstrated strong effects on controlling candidiasis. Here, we have proposed that the adhesion of cells through surface proteins is an important process. To allow the analysis of surface proteins, the concept of on-whole cell tryptic digestions were developed. This on-target approach allows for isolation of the peptides of interest, while greatly reducing digestion times.

To study the effects of Histatin 5 …

Sem Assessment Of The Enamel Surface After Debonding Of Ceramic Brackets, Anuja Kothari

Student Theses, Dissertations and Capstones

Objective: This study was conducted to compare the shear bond strengths, patterns of bond failure and enamel surfaces after debonding two ceramic brackets (ClearVu and Radiance Plus) and one standard metal bracket (Mini Uni-Twin). Background: Ceramic brackets are an esthetic alternative to metal brackets. The mean shear bond strength of ceramic brackets is significantly greater than for metal brackets. Excessive bond strength can result in pain upon debonding, damage to the bracket or permanent damage to the enamel including flaking, cracks or tooth fracture. Numerous studies have evaluated techniques to reduce the risk of enamel damage when debonding ceramic brackets, …

Treatment Outcomes Of Patients With Unilateral Class Ii Malocclusions: A Cbct Study, Brent Leggett

Loma Linda University Electronic Theses, Dissertations & Projects

Introduction: The purposes of this study were to determine the prevalence of Class II (Cl II) subdivision patients, to quantify the change in TMJ complex, and mandibular length after treatment was accomplished. Materials and Methods: Records of consecutive patients who sought orthodontic treatment at Loma Linda University (LLU) were screened. Only patients with dental Cl II subdivision that had completed treatment with pre- (T1) and post-treatment (T2) cone beam computed tomograms (CBCTs) available were evaluated for changes in the TMJ complex position related to Foramen Rotundum (TMJ-FR), joint spaces and mandibular length (condylion to genial tubercles). Comparisons were made between …

Comparison Of Maxillary Anterior Teeth Width To Facial Dimensions Among Three Races, Ewa Parciak

Loma Linda University Electronic Theses, Dissertations & Projects

Dental esthetics and tooth proportions have become increasingly relevant in dentistry, as the cosmetic demands of patients increase. Determining the appropriate esthetic dimensions of the maxillary anterior teeth is an important aspect in fixed and removable prosthodontics. The proportion of facial structures and the relationship between facial measurements and natural teeth could provide additional guidance in selecting denture teeth as well as in determining ideal teeth size for fixed restorations. There have been many publications on this topic, however, the subjects in these studies have been mostly selected from the Caucasian race. With the increasing diversity of the American population …

Morphological Changes In Alveolar Bone Following Orthodontic Space Closure, Ninette Hacopian

Loma Linda University Electronic Theses, Dissertations & Projects

Introduction: Changes occurring in anterior alveolar bone following orthodontic tooth movement are controversial. Some research has shown that orthodontic tooth movement results in the adaptation of cortical bone; others have shown that it results in dehiscence and fenestrations.

Purpose: The purpose of this study was to evaluate the alveolar bone changes around maxillary anterior teeth following orthodontic space closure.

Materials and Methods: Cone beam computed tomograms (CBCTs) of non-growing patients who received orthodontic treatment with bilateral maxillary premolar extraction were evaluated. Only patients with at least 5 degrees of changes in maxillary incisor (U1) to palatal plane angle were included …

Effect Of Diamond Bur And Root Canal Irrigants On Retention Of Fiber Posts, Rami Jekki

Loma Linda University Electronic Theses, Dissertations & Projects

Esthetic glass fiber-reinforced posts are being used more often. The most commonly reported complication associated with these posts is debonding. Dentine conditioning with solutions such as EDTA or MTAD results in removal of smear layer and might improve the retention of posts to root canal dentin. Therefore, the purpose of this study was to investigate the effect of cleansing the post space with MTAD or EDTA on the bond strength of glass fiber-reinforced posts, when a diamond roughening instrument is used prior to cementation with self-adhesive resin cement. Forty-five (n=15) extracted human premolar teeth were sectioned at the cemento-enamel junction …

Are Bone Turnover Markers And Vitamin D Levels Associated With Frequency Of Complete Denture Relines?, Shweta Puri

Loma Linda University Electronic Theses, Dissertations & Projects

PURPOSE: To compare groups of subjects with varying frequencies of complete denture relines in terms of their bone turnover markers; C-terminal Telopeptide (C-Tx), Osteocalcin (OCN) and Vitamin D (25 OH-Vit D) levels to determine the presence of and the degree of association between them.

METHODS: This study was designed as a cross-sectional study wherein a retrospective chart review of three hundred twenty four edentulous subjects with history of complete denture use for at least a year was performed to determine history and frequency of their complete denture relines. A total of 100 patients were enrolled. After consent was obtained, each …

Prevalence And Rationale Of Orthodontic Extractions At Loma Linda University, Teresa T. Tran

Loma Linda University Electronic Theses, Dissertations & Projects

Aim: The purpose of this study was to determine the prevalence of orthodontic extraction cases and the rationale for the decision-making. Materials & Methods: The records of consecutive patients who had completed orthodontic treatment at Graduate Orthodontic Clinic, Loma Linda University School of Dentistry, between January 2010 and December 2014 were reviewed for prevalence of orthodontic extractions. Cone beam computed tomograms (CBCTs) of patients, that had extraction(s) prescribed as part of orthodontic treatment, were analyzed dentally and skeletally. Patients were then categorized according to the degree of crowding in each dental arch. A one-way ANOVA (Kruskal-Wallis) test and …

Tooth Length Measurements On 3t Mr Images: A Retrospective Study, Kevin G. Murray

Loma Linda University Electronic Theses, Dissertations & Projects

Introduction: The purpose of this study was to determine if MRI can be used to accurately measure tooth lengths. Methods: MRI tooth length measurements were compared with “actual tooth lengths” as measured on CBCT scans. Twenty three subjects received two scans (one CBCT and one MRI). Tooth length was measured and compared between the resultant images. Intraclass correlations were used for statistical analysis. Results: Tooth length measurements made on MRI scans showed moderate to almost perfect agreement with tooth length measurements made on CBCT scans. Higher levels of agreement were present in the maxillary arch compared to the mandibular arch. …