Medicine and Health Sciences Commons^™

A Dynamic Mechanical Method To Assess Bulk Viscoelastic Behavior Of The Dentin Extracellular Matrix, Yvette Alania, Mariana Cavalcante Dos Reis, Joo-Won Nam, Rasika S. Phansalkar, James Mcalpine, Shao-Nong Chen, Guido F. Pauli, Ana K. Bedran-Russo

School of Dentistry Faculty Research and Publications

Objectives

To develop a protocol for assessment of the bulk viscoelastic behavior of dentin extracellular matrix (ECM), and to assess relationships between induced collagen cross-linking and viscoelasticity of the dentin ECM.

Methods

Dentin ECM was treated with agents to induce exogenous collagen cross-linking: proanthocyanidins (PACs) from Vitis vinifera – VVe, PACs from Pinus massoniana - PMe, glutaraldehyde – (GA), or kept untreated (control). A dynamic mechanical strain sweep method was carried out in a 3-point bending submersion clamp at treatment; after protein destabilization with 4 M urea and after 7-day, 6-month, and 12-month incubation in simulated body fluid. Tan δ, …

Removal Of Water Binding Proteins From Dentin Increases The Adhesion Strength Of Low-Hydrophilicity Dental Resins, Ana Paula Farina, Doglas Cecchin, Cristina M. P. Vidal, Ariene A. Leme-Kraus, Ana K. Bedran-Russo

School of Dentistry Faculty Research and Publications

Objectives

To investigate the role of proteoglycans (PGs) on the physical properties of the dentin matrix and the bond strength of methacrylate resins with varying hydrophilicities.

Methods

Dentin were obtained from crowns of human molars. Enzymatic removal of PGs followed a standard protocol using 1 mg/mL trypsin (Try) for 24 h. Controls were incubated in ammonium bicarbonate buffer. Removal of PGs was assessed by visualization of glycosaminoglycan chains (GAGs) in dentin under transmission electron microscopy (TEM). The dentin matrix swelling ratio was estimated using fully demineralized dentin. Dentin wettability was assessed on wet, dry and re-wetted dentin surfaces through water …

Effect Of Dentin Biomodification Delivered By Experimental Acidic And Neutral Primers On Resin Adhesion, Lívia Tosi Trevelin, Yvette Alania, Mathew Mathew, Rasika S. Phansalkar, Shao-Nong Chen, Guido F. Pauli, Ana K. Bedran-Russo

School of Dentistry Faculty Research and Publications

Objectives

Proanthocyanidins (PACs) are biocompounds mimicking native collagen cross-links. The effective and practical delivery of any biocompound is pivotal for clinical usage. The aim was to investigate the dentin biomodification and effective formation of dentin–resin biointerfaces of two highly bioactive PAC-rich extracts, Vitis vinifera (Vv) and Camellia sinensis (Cs), delivered using neutral (NP) or acidic (AP) rinse-out primer approaches.

Methods

The depth of dentin demineralization (optical profilometry), dentin biomodification (apparent modulus of elasticity, collagen auto-fluorescence) and properties of dentin–resin interfaces (microtensile bond strength - μTBS, and micro-permeability) were investigated. NP consisted of either 15% Vv or Cs applied for …

The Adverse Effects Of Radiotherapy On The Structure Of Dental Hard Tissues And Longevity Of Dental Restoration, Miguel Angel Munoz, Carolina Garin-Correa, Wilfredo Gonzalez-Arriagada, Ximena Quintela Davila, Patricio Haberle, Ana K. Bedran-Russo, Issis Luque-Martinez

School of Dentistry Faculty Research and Publications

Purpose:

The main goal of this study was to evaluate the impact of different ionizing radiation doses on the mineral (carbonate/phosphate ratio, crystallinity index [CI]) and organic (amide III/phosphate, amide I sub-band ratios) structures, as well as the microhardness, of enamel and dentin, along with their influence on the bonding strength stability of the etch-and-rinse (ER) and self-etch (SE) dental adhesive strategies.

Materials and methods:

Enamel and dentin human tissue specimens were irradiated (with 0, 20, 40, and 70 Gy radiation doses, respectively) and sectioned to perform an attenuated total reflection-Fourier transform IR spectroscopy assay (ATR-FTIR) and the Vickers microhardness …