Medicine and Health Sciences Commons^™

A Cell Culture Model To Study Tobacco Associated Oral Cancer Progression And Treatment Resistance, Amanda Busch

Theses & Dissertations

Head and Neck Squamous cell carcinoma is the 6^th most common cancer in the world. Risk factors for HNSCC include alcohol and tobacco consumption, infection with Human Papilloma Virus, and exposure to environmental toxins. Unlike many other cancers, the incidence of HNSCC is growing, yet the treatment options and outcomes have not been significantly improved for decades. An increased understanding of the molecular mechanisms of these cancers is needed for improvement in early diagnosis, enhanced treatment effectiveness, and cancer prevention. 4-nitroquinoline 1-oxide (4NQO) is a well-tested, tobacco mimicking, carcinogen. 4NQO produces similar histological and chemical changes found in human …

Secretion Of Salivary Proteins And Their Interactions, Karla Tonelli Bicalho Crosara

Electronic Thesis and Dissertation Repository

Saliva’s clinical application for disease diagnosis and monitoring is limited by incomplete knowledge of salivary protein interactions, the effect of stimulation on the salivary proteome, and if these factors impact protein identification. This thesis expands knowledge of the salivary interactome and effects of stimulation intensity and duration on parotid saliva’s proteome. First, previous in vitro studies identified 43 proteins in the histatin 1-protein network and demonstrated histatin 1’s increased stability in whole saliva when interacting with amylase. We hypothesized that protein-protein-interaction databases could enlarge the histatin 1-protein network. A comprehensive histatin 1-protein network was created using STRING database, merging previous …

Dental Hygienists’ Practices, Attitudes, And Confidence In Providing Care To Child Patients With Autism Spectrum Disorder (Asd), Fatimah Abdulrahman Alshehri

Dental Hygiene Theses & Dissertations

Problem: Given the growing number of children diagnosed with autism spectrum disorder (ASD) over the past decades and their significant need for access to dental care, it becomes essential to have dental hygienists adequately prepared to provide care to child patients with ASD. This study aimed to assess dental hygienists’ practices, attitudes, and confidence levels in providing care for child patients with ASD. Methods: An 18–item, adapted survey was validated and administered to participants at the Virtual ODU Continuing Education Annual Conference. Data was collected via Qualtrics® from 187 dental hygienist attendees. The survey domains studied included demographics, practice settings, …

Activated Charcoal Versus A Chemical Whitening Agent: Effect On Human Enamel, Hien Thai Doan

Master's Theses (2009 -)

Objective: For many years, the conventional method to achieve a white smile has been chemical regimens such as peroxide compounds. Previous studies suggest risks associated with chemical procedures, so many are seeking out alternative options such as utilizing activated charcoal. Since this new method is rapidly trending, there have not been many scientific studies on how utilizing activated charcoal can affect the mechanical/physical properties of tooth structure. The primary objective of this study was to investigate the effect of activated charcoal on enamel’s mechanical/physical properties and compare it to a conventional tooth whitening method (carbamide peroxide). Materials and Methods: Extracted …

Force Comparison Of Rotational Tooth Movements For Loop-Design And Traditional Aligners, Robert Olsen

Loma Linda University Electronic Theses, Dissertations & Projects

Introduction

This study aimed to evaluate the force (F) and torque (Tq) exerted on a rotated canine and molar tooth by two different aligner systems. An ArchForm loop clear aligner (AFL) was compared to a traditional clear aligner (T) with the force (F) and torque (Tq) compared. A reduction in F and Tq with AFL aligners could possibly decrease the incidence of Orthodontic Root Resorption (ORR), while also creating more effective forces for tooth movement.

Materials and Methods

Two types of T aligners varying by material thickness were compared to two types of AFL aligners varying by loop width. All …

Maxillary Central Incisor Rr In Hispanic And Non-Hispanic White Patients With Rme, Madison Healy

Loma Linda University Electronic Theses, Dissertations & Projects

Introduction: Apical root resorption is one of the most common adverse effects of orthodontic treatment. Forces used during orthodontic tooth movement can cause permanent loss of apical surfaces of teeth and although most reported cases of root resorption are asymptomatic, the process is irreversible and often unpredictable. Thus, it is of clinical interest to investigate which patients may be at greatest risk.

Purpose: This study utilized Cone Beam Computed Tomography (CBCT) to evaluate whether rapid maxillary expansion (RME) in Hispanic patients increased the severity of orthodontically induced apical root resorption.

Materials and Methods: Pre-treatment (T1) and post-treatment …

The Effect Of Interproximal Home Regimens Of Inflammatory Biomarkers In Periodontal Maintenance Patients, Grace Moore

Theses & Dissertations

A survey of dental hygienists in general practice (DHGP; n = 74) and periodontal practice (DHPP; n = 62) indicated a difference (p £ 0.04) in preference for interproximal cleaning with oral irrigators (DHGP) and interproximal brushes (DHPP) during periodontal maintenance therapy (PMT). The purpose of the subsequent blinded, randomized controlled clinical trial was to compare the effect of interproximal cleaning aids on reducing inflammation in posterior pockets during PMT. Seventy-six patients on PMT with a 5-7 mm interproximal probing depth were randomly allocated to one of three groups: 1) interproximal brush with tracking device (IB + TD; n=23), 2) …

An Assessment Of Pregnant Women's Value And Utilization Of Oral Health Care During Pregnancy, Angelica M. Sanchez

Dental Hygiene ETDs

The intention of this survey was to examine the value and utilization of dental services during pregnancy, to identify the value being placed on oral health and dental services during pregnancy. Also, to assess if the women received oral health education from obstetric providers. Pregnancy temporarily increases the risk of periodontal diseases, the prevalence of periodontal diseases is very high during pregnancy, 60-75% of women can be affected. Previous literature suggests that poor oral health is linked to adverse pregnancy outcomes such as pre-term births, low birth weights, and pre-eclampsia. Data was obtained and collected using REDCap. Utilizing the collected …

Extrinsic And Intrinsic Factors Influencing Homeostasis And Repair In Oral Tissues, Georgia Nikoloudaki

Electronic Thesis and Dissertation Repository

The oral tissues, including gingiva and buccal mucosa, heal rapidly with minimal scar formation. Although in the oral cavity, the palatal mucosa is associated with excessive scarring in response to injury. In this thesis, first, I investigated the complex relationship between cells and their microenvironment using wound healing models in which extracellular matrix composition and mechanical stiffness affect cellular responses during acute wound repair. I also determined how intrinsic differences of cells recruited during healing contribute to wound resolution.

In Chapter 2, I first investigated cell-extrinsic factors, specifically the influence of the pro-fibrotic matricellular protein periostin on palatal healing. Using …

Evaluation Of Neonatal Ankyloglossia, Ellen M. Ray

Dissertations

Problem Ankyloglossia occurs when the tongue cannot extend past the gums or lips due to frenulum restriction. In breast feeding, proper movement of the tongue around the nipple is hindered causing difficulty for the infant to obtain a proper latch. This may result in slow weight gain (SWG), failure to thrive (FTT), and dehydration due to poor milk production. Evaluating for ankyloglossia is difficult, however, the Hazelbaker Ankyloglossia Tool for Lingual Frenulum Function (HATLFF) may be of benefit.

Method Observational descriptive design utilizing a retrospective medical record review. The HATLFF was utilized in full term neonates suspected of ankyloglossia in …

The Accuracy And Sensitivity Of Abo Electronic Cast Radiographic Program Orthoshare 360 Compared To Manual Measurements, Lisa Nguyen

Master's Theses (2009 -)

Objective: The objective of this study was to assess the accuracy, validity, and reliability of measurements obtained by MotionView's Ortho Share 360 program compared to traditional manual measurements of 3D printed models using a standardized ABO measuring gauge. In detail, the study aimed to evaluate the difference in total ABO Cast Radiographic-Evaluation (CRE) score and individual differences among six ABO CRE parameters. The null hypothesis is that there are no statistically different scores between digital and manual measurements. Methods: 33 samples were measured based on standardized parameters determined by the ABO. The first measurements were obtained manually using ABO measuring …

Mechanism Of Mechanical Vibration In Enhancing Orthodontic Retention- Analysis Of Global Gene Expression, Shuwei Wang

Master's Theses (2009 -)

Introduction: Low-magnitude, high-frequency (LMHF) mechanical vibration has been proposed as a possible adjunctive retention method after orthodontic treatment. In this study, we explored the mechanism of using LMHF vibration to improve bone formation during retention phase. We aimed to provide insight of global gene expression and cellular activities of PDL cells under tension, as well as identify novel biomarkers for induced osteogenesis by using LMHF mechanical vibration as adjunct tool in retention phase. Materials and Methods: Human PDL cells (hPDLCs) were seeded in 6-well FLEXCELL culture plates. The wells were assigned to four experimental groups as control, stretching (ST), vibration …

Color Characterization Of Color Changing Orthodontic Adhesives, Megan Fried

Master's Theses (2009 -)

Objective:A popular product to facilitate removal of excess adhesive during bonding of orthodontic appliances is a color changing adhesive. Before polymerization, these materials are a bright color but upon curing, manufacturers claim they become translucent. After debonding, there is often resin embedded within the enamel, so the appearance of these materials after polymerization can affect the esthetics of a patient’s dentition. The purpose of this study was to analyze the color characteristics of various orthodontic color changing adhesives and determine if there is a significant difference in color changing capabilities and in translucency after polymerization between products. Methods:Grengloo and Blugloo …

Autoignition And Sooting Characteristics Of Iso-Octane And Ethanol In An Optical Rapid Compression Machine, Richard John Kempf

Master's Theses (2009 -)

To effectively reduce particulate emissions and maximize the effectiveness of renewable fuels entering the industrial and transportation markets, an accurate understanding of the combustion characteristics of these fuels is a necessary undertaking. Typical engines used in the heavy-duty engine industry primarily rely on mixing-controlled combustion, which has the potential to produce soot under locally rich combustion conditions. A rapid compression machine (RCM) is an experimental reaction vessel used to investigate the ignition and pollutant behaviors of fuels at compressed conditions relevant to engine studies. In this study, an RCM has been prepared with line-of-sight optical accessibility such that a laser …

The Effect Of Tray Design And Impression Material Consistencies On Accuracy Of Complete Arch Implant Supported Fixed Dental Prostheses Master Casts, Lujain Kurdi

Master's Theses (2009 -)

Purpose of this Study: The purpose of this study is to determine the accuracy and precision of complete arch implant supported fixed dental prosthesis impressions made with a low viscosity PVS while supporting the splinted impression copings with heavy viscosity PVS on a reference model simulating an all-on-4 prosthesis using both stock and custom trays. Material and Methods: A model was scanned to act as the reference model. The splinting device and custom tray were printed using FormLAb printer and custom resin tray material. Four group were included in the study. Stock tray with and without support and custom tray …

Evaluation Of Damon Psl And Conventional Mbt Brackets In Leveling And Alignment Efficiency, Jacob Beckstrand

Master's Theses (2009 -)

Objective: Ormco has proposed that the low-friction design of their self-ligation brackets allows for more efficient alignment of crowded teeth with less incisor proclination and faster treatment times compared to conventional MBT bracket systems. The purpose of this study is to examine the hypothesis that there is no difference between Damon PSL and conventional MBT brackets in factors related to leveling and alignment in-vitro. Methods:Damon Q2 .022” slot brackets and 3M Unitek .022” slot MBT brackets were bonded to 2 sets of typodont teeth. Typodont teeth were placed into 8 upper and lower Class I crowding wax forms (Kilgore, USA) …

Titanium Particles Within Mucosa During Non-Surgical Implant Debridement, Holvin Louie

Loma Linda University Electronic Theses, Dissertations & Projects

Titanium implants affected by peri-implantitis lesions are often treated non-surgically with various instruments, which may produce titanium particles that can become lodged within the peri-implant mucosa. Thus, a foreign body reaction may ensue. The aim is to assess the embedment of titanium particles into soft tissues from titanium implants via a stainless-steel ultrasonic scaler (SSU) versus a titanium hand scaler (TH). Sand-blasted, large grit, acid-etched surface-treated titanium implants were implanted into pig legs after creating peri-implantitis defects. Each implant was subjected to only one instrument type. Sequential strokes were conducted followed by thorough irrigation with sterile water. Soft tissue curettage …

An Exploratory Look Into The Effects Of Mississippi's Population Decline On Rural Dental Care, Allen Bailey Saffold

Honors Theses

Literature reviews and interviews with dentists working in rural Mississippi counties examined the effects of population decline on rural dental care in Mississippi. With the state’s continual population decline, loss of industry follows a loss of people. Through a cascade of effects, this can further reduce underserved areas’ access to dental care. In order to address the issue of rural Mississippi continuing to be underserved, policies need to be implemented that will make rural practice at least enticing if not desirable.

An Exploration Of Low-Income Dental Services In The Tri-Cities, Macie Proffitt

Undergraduate Honors Theses

Dental care has great importance in the overall health of every individual. Many do not realize the effect oral health plays in their overall health and many areas in the United States do not have access to the dental care needed to obtain proper healthcare standards. In many of these areas, such as the Appalachian region, dental services for low-income citizens are limited. Specifically, in the Northeast Tennessee region, these dental services are severely limited. Washington County and Hawkins County of Northeast Tennessee are specific underserved areas in the Appalachian region. The purpose of this project was to explore the …

Dental Students' Perceptions Of Dental Hygienists' Education And Scope Of Practice, Cynthia Metzger

Electronic Theses and Dissertations

Oral health is strongly correlated to systemic health in the United States. Dental professionals play a significant role in addressing the oral health and prevention of disease for populations in the United States. Because many areas in the United States lack access to dental care, the federal government considers dental hygienists part of the solution. It is now paying attention to restrictive dental hygiene scope of practice rules in each state. State dental boards, comprised mainly of dentists, make these rules based on their perception of dental hygienists' lack of education. This study explored fourth-year dental students' perceptions of the …

Quantification Of Dental Fissures Through Occlusal Topography Analysis, Duru Erkan

Biological Sciences Undergraduate Honors Theses

Narrow, deep, and elaborate dental fissures are widely believed to increase the predisposition of enamel to develop carious lesions as these surfaces are thought to be ideal for cariogenic bacteria to adhere to. Therefore, dentists prescribe sealants as a preventative measure for patients with such fissures to avoid tooth decay. Yet to date, there is no objective data supporting the notion that fissure morphology actually affects caries susceptibility, nor is there a quantifiable, clinically practical method to characterize fissure patterns expected to increase risk of caries disease.

In this study, three new methods to quantitatively characterize fissure pattern in mandibular …

The Effect Of Cognitive Status And Residency On Oral Health In Patients With Dementia, Nicole Stephens

Dissertations & Theses (Open Access)

Poor oral health is a predictor of cognitive decline in elderly populations and has been shown to precede dementia. As cognitive decline progresses, patients are likely to move from the community into nursing facilities. We hypothesize that severity of dementia and residency type will impact the oral health of patients with dementia. Fifty-two participants of two dementia levels were recruited from the UTHealth Neurocognitive Disorders Center and two Houston-area nursing homes. A standardized oral health assessment, plaque index, and oral bacteria analysis determined participants’ oral health status. Further, data was collected on participants’ medical history, oral hygiene habits, dietary habits, …

Disaster Victim Identification Competencies: A Comparison Of Dental Hygiene And Dental Assistant Students, Samantha Clara Vest

Dental Hygiene Theses & Dissertations

Purpose: The purpose of this study was to assess cognitive and affective learning outcomes of allied dental students following a disaster victim identification (DVI) multi-media learning experience. Methods: A convenience sample (n=41) of senior dental hygiene and dental assisting students from two institutions completed an IRB-exempt DVI learning experience which included a multimedia intervention with a pretest/posttest survey to assess cognitive and affective changes. The multimedia intervention was a PowerPoint presentation with educational text, audio/video recordings, and presented in a virtual synchronous format by one of the researchers. After completing the learning experience, participants were presented with 10 sets of …

Crispr-Cas In The Field Of Dentistry: A Comprehensive Collection Of The Potential Uses Of Crispr-Cas9 In Dental Health Care, Lexi Elaine Bales

Chancellor’s Honors Program Projects

No abstract provided.

Aggregatibacter Actinomycetemcomitans Exploits And Modulates The Immune Response By Human Neutrophils For Survival In The Anaerobic Environment., Hazel Ozuna

Electronic Theses and Dissertations

Aggregatibacter actinomycetemcomitans (Aa) is a Gram-negative facultative anaerobe and an opportunistic oral pathogen, strongly associated with localized periodontitis and other inflammatory diseases. Periodontitis is a chronic inflammation of the periodontium resulting from the inflammatory response of the host towards the dysbiotic microbial community present at the gingival crevice. The host immune response creates a hostile environment for microorganisms; therefore, it is important for Aa to be able to regulate the necessary genes to survive and thrive in such an environment. Aaexpresses several virulence factors such as a cytolethal distending toxin (Cdt), lipopolysaccharide (LPS) and leukotoxin A (LtxA), …

Differential Expression Of Cellular And Exosomal Microrna Isolated From Oral Cancer Cells And Their Resistance To Chemotherapy, Brady Petersen

UNLV Theses, Dissertations, Professional Papers, and Capstones

Introduction: MicroRNAs are abundant small non-coding RNA with a variety of known functions, including transcriptional activation and inhibition. Recent evidence has suggested that microRNA expression may influence the responsiveness of some cancers to chemotherapy, including liver and lung cancers. Some evidence has now suggested that specific microRNAs, such as miR-21, miR-155, and miR-375, may influence oral cancer responsiveness to chemotherapy – although much remains to be discovered.

Objective: Based upon the lack of evidence in this area, the primary objective of this study was to evaluate microRNA expression and responsiveness among oral cancers.

Methods: Five commercially available oral cancer cell …

Comparative Expression Of Exosome-Derived Dental Pulp Stem Cell (Dpsc) Microrna, Matthew Hunsaker

UNLV Theses, Dissertations, Professional Papers, and Capstones

Introduction: Dental pulp stem cells (DPSC) are pluripotent stem cells capable of differentiating into several tissue types. Several studies have demonstrated DPSC can be extracted from deciduous and permanent teeth, including third molars (wisdom teeth) - however, much less is known regarding the factors that determine and regulate pluripotency and differentiation responsiveness among these harvested DPSC. Recent evidence has suggested small, non-coding microRNAs mediate (in part) differentiation and self-renewal among some stromal and hematopoetic stem cells, although much remains to be discovered regarding microRNA regulation of DPSC phenotypes. Based upon this information, the overall goal of this study was to …

Dental Morphology In A Juvenile Mammal From The Late Cretaceous Of South America: Tooth Eruption And Development., Kayla E Newton

Electronic Theses and Dissertations

Meridiolestids are a diverse and distinct clade of Cretaceous–Miocene South American dryolestoid mammals ranging in size from small shrew-sized insectivores to large dog- sized omnivores. Cronopio dentiacutus from the Cenomanian La Buitrera locality, Rio Negro Province, Argentina (Candeleros Fm.) is the oldest and best known of them, represented by skull material and associated jaws. A juvenile skull as attributed to Cronopio dentiacutus, based on tooth size and morphology, was collected from the type locality. The specimen includes the skull and both articulated lower jaws. The fossil is poorly ossified and partially flattened; CT scanning reveals most of the dentition …

Development Of A Non-Invasive Dna Extraction Method From Teeth, Monalisa Heng

Honors Theses

Forensic dentistry has evolved from simple morphological investigations to genetic and molecular analysis. One of the most important techniques is DNA analysis which requires viable samples from an individual or crime site. The orofacial region can be a good source for DNA samples because the tissues can resist environmental, thermal, electrical, and mechanical challenges. Teeth are especially useful as a source of genetic material because the DNA located in the pulp is surrounded by protective mineral tissue. The DNA in the pulp tissue is currently accessed through destructive methods involving crushing, pulverization, and splitting the tooth. Unfortunately, destructive methods destroy …

Impact Of Interleukin-34 On The Promotion Of Bone Osteolysis And Neuroinflammation In Experimental Models Of Alzheimer’S Disease, Anny Ho

All HCAS Student Capstones, Theses, and Dissertations

Alzheimer’s disease (AD) is a growing health concern and is the most common type of dementia worldwide. Emerging evidence indicates that aggregated amyloid-beta (Aβ) peptides, one of the hallmark features of AD neuropathology, can increase RANKL-mediated osteoclast activity leading to the prevalence and severity of inflammatory osteolysis, e.g., osteoporosis and periodontal bone loss. Conversely, osteolytic lesions are associated with increased risk of dementia diagnosis indicating that there is a direct link between dementia and inflammatory osteolysis. It was demonstrated that the neuronal cells primarily produce interleukin-34 (IL-34) and microglia, macrophages, and osteoclast precursors express colony-stimulating factor 1 receptor (CSF-1R), a …