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Full-Text Articles in Medicine and Health Sciences

Variability Of Two Sampling Methods In Plaque Samples, Kuei C. Hsu Jan 2008

Variability Of Two Sampling Methods In Plaque Samples, Kuei C. Hsu

All ETDs from UAB

Introduction: Dental caries remain the most common infectious disease in children, and is in fact, on the rise in the U.S. among 2 to 5 year olds. Among hundreds of oral bacteria, mutans streptococci (MS) are considered the major species associated with dental caries. Studies have shown that increased MS in saliva or plaque is an important indicator for caries. The use of standard plate count (SPC) from plaque samples particularly yield variable quantitative results because of complexities of sample collection and processing. Objectives: This study was undertaken to determine collection reproducibility in plaque samples from permanent first molars using …


Endodontic Sealers-Their Properties And Effects On Fiber Post Retention, Farrah Huthail Najib Jan 2008

Endodontic Sealers-Their Properties And Effects On Fiber Post Retention, Farrah Huthail Najib

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Zinc oxide eugenol sealers have been used clinically for several years. Glass ionomer sealers were introduced due to their adhesion to the dental hard tissues and excellent sealing ability. During the process of endodontic treatment a post, core and final restoration is required for tooth restoration. Recently fiber reinforced composite posts have become more popular because of their mechanical properties and esthetics. However, fiber posts have to be adhesively cemented into root canal which can be affected by the endodontics sealers. The purpose of this study was to evaluate the effect of eugenol and non-eugenol containing sealers on the retention …


Bone Formation Around Implants In Adult Transgenic Mice With Selective Runx2-Ii Deficiency, Jaime Rodrigo Rivera Jan 2008

Bone Formation Around Implants In Adult Transgenic Mice With Selective Runx2-Ii Deficiency, Jaime Rodrigo Rivera

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Titanium-aluminum-vanadium (Ti-6Al-4V) tacks were placed in the tibiae of 10 female wild type (Runx2 flox/+ or Runx2 flox/flox) and heterozygous (Runx2+/-, ColII Cre) mice littermates to examine healing and bone response. Animals were divided into control and test groups of 5 each. A novel generation of Runx2 knock-out mice where the gene is only deleted in chondrocytes was used. Ten days after implant surgery, specimens were recovered and prepared for histological, histomorphometric, and micro-CT morphometric analysis. Histological examination revealed that wild type animals presented larger regions of bone formation and endochondral ossification when compared to heterozygous mice. Micro-CT morphometric analysis …


Oral Mucositis In Children Receiving Bone Marrow Transplantation, Francisco Jose Rodriguez Jan 2008

Oral Mucositis In Children Receiving Bone Marrow Transplantation, Francisco Jose Rodriguez

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Purpose: The purpose of this retrospective study was to identify the factors that influence the incidence, severity, onset, and resolution of oral mucositis in children undergoing bone marrow transplantation (BMT). Methods: One hundred and fifty medical records were reviewed of patients that received BMT. Daily values of white blood cell (WBC) counts were obtained to associate it’s correlation with the incidence, severity, onset, and resolution of oral mucositis. Age group and gender of the patients, type of bone marrow transplant, the use of methotrexate, the use of total body irradiation, and the previous treatment with chemotherapy were obtained to analyze …


Ca 2+ /Calmodulin-Dependent Protein Kinase Ii Regulates The Growth Of Human Osteosarcoma Cells In Vivo, Hyeran Choo Jan 2007

Ca 2+ /Calmodulin-Dependent Protein Kinase Ii Regulates The Growth Of Human Osteosarcoma Cells In Vivo, Hyeran Choo

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Osteosarcoma is the most predominant primary bone cancer among pediatric patients. The pathogenesis and mechanism of osteosarcoma, however, as yet remains unclear. This study investigated the hypothesis that Ca2+/calmodulin-dependent protein kinase II (CaMK II), activated by Ca2+ and its intracellular receptor, calmodulin, plays a critical role in the growth of human osteosarcoma cells. The inhibition of CaMK II with its pharmacologic antagonist KN-93 was used to investigate the role of CaMK II in the growth of human osteosarcoma cells because our preliminary data showed that =-CaMK II is expressed in several human osteosarcoma cell lines. To examine the in vivo …


A Case Series Study Of Vitapex Pulpectomy Treatment In Primary Teeth Ii, Hsin-Ju Hsieh Jan 2007

A Case Series Study Of Vitapex Pulpectomy Treatment In Primary Teeth Ii, Hsin-Ju Hsieh

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Purpose. The aim of this case series study was to conduct a long-term follow-up and review of subjects with previous Vitapex® pulpectomies, to evaluate outcomes clinically and radiographically, and to ascertain whether there was any interference with exfoliation of deciduous teeth or abnormal development of succedaneous teeth. Methods. This case series study was conducted by following target groups of pediatric patients who had received Vitapex® pulpectomies in their primary teeth. Data were collected by reviewing charts, identifying patients who met our criteria, and recalling them for follow-up appointments. After sufficient data were accumulated, survival analysis was applied and presented by …


Outcome Assessment On Skeletal Stability After Rigid External Distraction Osteogenesis In Cleft Lip And Palate Patients, Julio Enrique Tabarini Jan 2007

Outcome Assessment On Skeletal Stability After Rigid External Distraction Osteogenesis In Cleft Lip And Palate Patients, Julio Enrique Tabarini

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Purpose: Distraction osteogenesis (DO) using an external distraction device in the correction of maxillary hypoplasia (MH) in cleft lip and palate patients has been shown to be an effective and stable technique. The purpose of this study is to determine the outcome of skeletal stability for this procedure using the Rigid External Distraction (RED) System in patients with cleft lip and palate (CLP). Materials and methods: Twenty CLP patients with moderate to severe maxillary hypoplasia were treated between June 2000 and June 2006. Their average age at the time of the surgery was 12.6 years. DO was started after a …


Barrier Membranes For Ridge Augmentation - Is There An Optimal Pore Size?, Rajesh Gutta Jan 2007

Barrier Membranes For Ridge Augmentation - Is There An Optimal Pore Size?, Rajesh Gutta

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Background: - Alveolar bone loss is often a sequelae of edentulism. However, several reconstruction procedures with bone grafts and barrier membranes are used to restore the lost bony architecture. The value of titanium mesh barriers has been shown to be reliable both in vertical and horizontal ridge augmentation procedures. However, there is a paucity of literature supporting the role of pore size of barrier membranes in preventing soft tissue ingrowth during ridge augmentation procedures. There are multiple reports describing a layer of soft tissue with varying thickness beneath the mesh and adhering to the newly regenerated bone. The objective of …


Isolation And Characterization Of Human Periodontal Ligament Stem Cells, Isabel C. Gay Jan 2007

Isolation And Characterization Of Human Periodontal Ligament Stem Cells, Isabel C. Gay

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The periodontal ligament (PDL) is thought to include progenitor cells capable of forming fibroblasts, osteoblasts and cementoblasts. However, characterization of PDL stem cell populations (SC) remains undetermined due in part, to the diversity of cell types that conform to the periodontal apparatus. Objective: To isolate and characterize PDLSC and assess their pluripotent capabilities to differentiate into bone, cartilage and adipose cell types. Methods: PDL was scraped from human teeth, enzymatically digested, and cells stained for STRO- 1 (SC marker) using fluorescent immunohistochemistry. Positive cells were FACS sorted and expanded in culture. Human bone marrow SC (BMSC) served as a positive …


Surface Reproducibility Of Impression Materials, Amit Shah Jan 2007

Surface Reproducibility Of Impression Materials, Amit Shah

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The inability to absorb or release moisture limits the ANSI/ADA specification 19 stainless steel mold from accurately mimicking tooth structure and oral conditions while evaluating in vitro detail reproduction of elastomeric impression materials. Moreover, studies utilizing this mold have been graduated at room temperature, not normal mouth temperature. Bovine teeth, being similar to human teeth in microstructure and composition, are the closest substitutes that can be used to evaluate surface detail reproducibility of impression materials. The objectives of this study were (1) to develop a new bovine in vitro test model at controlled temperature and humidity in order to simulate …


Vitamin D Receptor Deficiency And Postnatal Tooth Formation, Xueming Zhang Jan 2007

Vitamin D Receptor Deficiency And Postnatal Tooth Formation, Xueming Zhang

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The reduction of caries is one of the major objectives of Healthy People 2010 (USDHHS, 2000). For individuals at risk for caries, early identification and interception before the occurrence of the disease are crucial to preventing the cariogenesis. However, host genetic variants that compromise the mineralization of dental enamel and dentin are unclear. The vdr mutation has been reported to be associated with bone loss. Therefore, it is likely that vitamin D resistance may affect dental enamel and dentin formation. The objective of this study was to investigate the effects of VDR deficiency on postnatal tooth formation in a vdr …


The Effect Of Cementation Technique On The Retention Of Adhesively Cemented Prefabricated Posts, Carlos A Polo Montes Jan 2007

The Effect Of Cementation Technique On The Retention Of Adhesively Cemented Prefabricated Posts, Carlos A Polo Montes

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Adhesively cemented prefabricated posts are frequently employed for the restora-tion of endodontically treated teeth. Bonding agents may improve the post and core per-formance. Correct dentin bonding procedures are a critical factor in the bonded post re-tention, but adequate bonding in the canal space has been reported difficult to achieve. The operator is unable to visualize the depth of conditioner gel penetration into the canal, and its removal cannot be easily confirmed. Additional potential problems include excess water removal after rinsing the canal, inadequate primer coverage and/or removal, and poor curing methods for the resin luting agent. A technique to avoid …


In Vitro Compressive Fracture Resistance Of The Human Maxillary First Premolar With Different Mod Cavity Design And Restorative Materials, Wen-Chou Wu Jan 2007

In Vitro Compressive Fracture Resistance Of The Human Maxillary First Premolar With Different Mod Cavity Design And Restorative Materials, Wen-Chou Wu

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Cuspal failures of MOD (mesial-occlusal-distal) restored teeth have been constantly observed in clinic especially those restored with amalgam. As the pulpal depth and isthmus width of the preparation increases, the strength decreases. A number of studies have investigated the adhesive technique which is intended to increase the fracture resistance by bonding the tooth structure. The results of the studies are controversial because of the differences of research design among studies. The purpose of this study is to evaluate the fracture resistance of the human maxillary first premolar restored with condensable composite (Surefil, Dentsply) and CEREC 3D ProCAD leucite-reinforced ceramic inlays …


The Impact Of A Community Health Advisor-Based Intervention On Self-Reported Frequency Of Dental Visits In A Rural, Low-Income African American Alabama Community, Stephen J. Clarke Jan 2007

The Impact Of A Community Health Advisor-Based Intervention On Self-Reported Frequency Of Dental Visits In A Rural, Low-Income African American Alabama Community, Stephen J. Clarke

All ETDs from UAB

Oral health disparities are wide and increasing for rural Southern African- Americans. This project trained 13 experienced Community Health Advisors (CHAs) from Uniontown, Alabama, in the basics of oral health, including regular dental visits, and assigned them to spread this information among their fellow community members. We tested whether a 1-year CHA-based oral health education intervention was associated with increased routine/preventive use of available dental services as measured by pre-post self-report, relative to a comparison community. We also hypothesized that any detected association between the intervention and use of dental services was mediated by attitudes toward dental visits. No direct …


Micro-Computed Tomographic Analysis Of Bone Healing Subsequent To Graft Placement, Preeti M. Chopra Jan 2006

Micro-Computed Tomographic Analysis Of Bone Healing Subsequent To Graft Placement, Preeti M. Chopra

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A MicroCT analysis of bone healing subsequent to graft (tri calcium phosphate/TCP) placement in the maxillary sinus prior to dental endosteal implant placement was the objective of the current study. Ten trephined rod shaped human bone cores were obtained three months after the placement of particulate graft material and the samples were stored immediately in 10% neutral buffered formalin. Using a Microcomputed tomography, the samples were evaluated at resolutions of 6 and 20μm. The thresholds for bone and graft material were determined using visual image, intensity (grey level) and histogram analyses. The graft and bone threshold was 235 greylevel and …