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Full-Text Articles in Medicine and Health Sciences
Regulated Phosphorylation Of The K-Cl Cotransporter Kcc3 Is A Molecular Switch Of Intracellular Potassium Content And Cell Volume Homeostasis, Norma C. Adragna, Nagendra B. Ravilla, Peter K. Lauf, Gulnaz Begum, Arjun R. Khanna, Dandan Sun, Kristopher K. Kahle
Regulated Phosphorylation Of The K-Cl Cotransporter Kcc3 Is A Molecular Switch Of Intracellular Potassium Content And Cell Volume Homeostasis, Norma C. Adragna, Nagendra B. Ravilla, Peter K. Lauf, Gulnaz Begum, Arjun R. Khanna, Dandan Sun, Kristopher K. Kahle
Pharmacology and Toxicology Faculty Publications
The defense of cell volume against excessive shrinkage or swelling is a requirement for cell function and organismal survival. Cell swelling triggers a coordinated homeostatic response termed regulatory volume decrease (RVD), resulting in K+ and Cl− efflux via activation of K+ channels, volume-regulated anion channels (VRACs), and the K+-Cl− cotransporters, including KCC3. Here, we show genetic alanine (Ala) substitution at threonines (Thr) 991 and 1048 in the KCC3a isoform carboxyl-terminus, preventing inhibitory phosphorylation at these sites, not only significantly up-regulates KCC3a activity up to 25-fold in normally inhibitory isotonic conditions, but is also accompanied by reversal of activity of the …
Loss Of Prolyl Carboxypeptidase In Two-Kidney, One-Clip Goldblatt Hypertensive Mice, Nadja Grobe, Orly Leiva, Mariana Morris, Khalid M. Elased
Loss Of Prolyl Carboxypeptidase In Two-Kidney, One-Clip Goldblatt Hypertensive Mice, Nadja Grobe, Orly Leiva, Mariana Morris, Khalid M. Elased
Pharmacology and Toxicology Faculty Publications
It is well documented that angiotensin (Ang) II contributes to kidney disease progression. The protease prolyl carboxypeptidase (PRCP) is highly expressed in the kidney and may be renoprotective by degrading Ang II to Ang-(1-7). The aim of the study was to investigate whether renal PRCP protein expression and activity are altered in two-kidney, one-clip (2K1C) Goldblatt hypertensive mice. Left renal artery was constricted by using 0.12 mm silver clips. Blood pressure was measured using telemetry over the eleven weeks of study period and revealed an immediate increase in 2K1C animals during the first week of clip placement which was followed …