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Hepatoma Cells Up-Regulate Expression Of Programmed Cell Death-1 On T Cells, Ji Chen, Gui-Qiang Wang
Hepatoma Cells Up-Regulate Expression Of Programmed Cell Death-1 On T Cells, Ji Chen, Gui-Qiang Wang
Pharmacology and Toxicology Faculty Publications
AIM: To investigate the effect of hepatoma cells on up-regulation of programmed cell death-1 (PD-1), and the function of PD-1 on T cells. METHODS: HepG2 or HepG2.2.1.5 cells were co-cultured with a lymphoma cell line-Jurkat cells. PD-1 expression was detected by flow cytometry. IL-2, INF-γ and IL-10 in culture supernatant were detected by enzyme-linked immunosorbent assay (ELISA). Cytotoxic action of T cells was determined by MTT reduction assay-direct mononuclear cell cytotoxicity assay. RESULTS: The PD-1 expression on Jurkat cells increased by 16.17% ± 2.5% and 17.43% ± 2.2% after HepG2 or HepG2.2.1.5 cells were co-cultured for 48 h. The levels …
Screening Assay For Enzymes Involved In Pathophysiology Using Seldi-Tof Mass Spectrometr, Khalid M. Elased, Mariana Morris
Screening Assay For Enzymes Involved In Pathophysiology Using Seldi-Tof Mass Spectrometr, Khalid M. Elased, Mariana Morris
Pharmacology and Toxicology Faculty Publications
The invention provides methods for quantifying enzymatic activity of an enzyme with a known substrate. The methods employ SELDI-TOF mass spectrometry, and are suitable, in particular, for assaying aspects of the renin-angiotensin system. The methods may be utilized to assess and/or monitor biological conditions associated with the renin-angiotensin system prior to the manifestation of known physiological and biomarkers for such conditions. The methods are suitable for analysis of pharmacological effectors of the renin-angiotensin system, and are particularly suitable for automation and high-throughput screening assay design.