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Full-Text Articles in Medicine and Health Sciences
The Mitochondrial Calcium Uniporter Regulator 1 (Mcur1) Matrix Domain Is A Self-Associated Multimer Sensitive To Divalent Cations, Naveed Siddiqui
The Mitochondrial Calcium Uniporter Regulator 1 (Mcur1) Matrix Domain Is A Self-Associated Multimer Sensitive To Divalent Cations, Naveed Siddiqui
Electronic Thesis and Dissertation Repository
Mitochondria are primarily appreciated for the generation of adenosine triphosphate (ATP), a chemical store of energy required by all cells. These organelles, however, also play key roles in apoptosis, autophagy and shaping cytosolic calcium (Ca2+) signaling via Ca2+ uptake into the mitochondrial matrix. This Ca2+ uptake is mediated chiefly via the mitochondrial Ca2+ uniporter (MCU), an inner mitochondrial membrane protein that oligomerizes to form a Ca2+ selective pore. MCU is regulated by several protein binding partners, including the recently identified MCU regulator-1 (MCUR1). MCUR1 stabilizes a higher order MCU heterocomplex through interactions with MCU …
Effects Of G Protein Signalling Modulator 3 On Cellular Signalling, Aneta A. Surmanski
Effects Of G Protein Signalling Modulator 3 On Cellular Signalling, Aneta A. Surmanski
Electronic Thesis and Dissertation Repository
G protein coupled receptors (GPCRs) promote G protein heterotrimer (Gα·GDP/Gbg) activation.GPCRsignalling is limited via G protein GTPase activity and b-arrestin-receptor interactions. G Protein Signalling Modulators (GPSMs) are proteins that may influence receptor signalling through G protein activity. GPSM3 modulates their activity by binding to Gai-GDP, limiting nucleotide exchange and preventing its re-association to Gbg. The impact of GPSM3 on signalling is unknown.We hypothesize that GPSM3 will decrease Gai-dependent signalling while promoting Gbg-dependent signalling in Gi-coupled GPCRs.
GPSM3 significantly inhibited b-arrestin recruitment to α2A-adrenergic and m-opioid receptors via a Gbg-dependent mechanism, …
A Chimeric Nucleobase - Phenylazo Derivative As An Intrinsic Nucleobase Quencher, Gyeongsu Park, Timothy Martin-Chan, Amer El Samm, Robert H.E. Hudson
A Chimeric Nucleobase - Phenylazo Derivative As An Intrinsic Nucleobase Quencher, Gyeongsu Park, Timothy Martin-Chan, Amer El Samm, Robert H.E. Hudson
Western Research Forum
Molecular beacons are important bioanalytical probes which are most often
constructed from a single-stranded oligonucleotide which has been labeled at
opposite termini with a fluorophore and a quencher. When the fluorophore and
quencher are in close proximity, no fluorescence is observed due to FRET
(Fluorescence Resonance Energy Transfer). DABCYL (4-dimethylaminoazobenzene-
4'-carboxylic acid) has been used as a quencher in the molecular beacon to absorbs
excitation energy from a fluorophore and to dissipate the energy as heat. However,
DABCYL is unable to form a base-pair and is conventionally placed as an overhanging
residue. This produces a derivative wherein the chromophore has …