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Full-Text Articles in Medicine and Health Sciences
Producing A Subunit Vaccine For Porcine Epidemic Diarrhea Virus, Zayn Khamis
Producing A Subunit Vaccine For Porcine Epidemic Diarrhea Virus, Zayn Khamis
Electronic Thesis and Dissertation Repository
Porcine epidemic diarrhea virus (PEDv) causes disease and mortality to piglets worldwide. Most vaccines used to combat the disease have been ineffective live attenuated virus vaccines. The goal of this project was to produce a plant-made subunit vaccine based off the membrane protein of the virus. This is the first time this protein has been produced in plants. An elastin-like polypeptide fusion membrane protein accumulated up to 0.8 mg/g of fresh leaf weight when transiently expressed in Nicotiana benthamiana. Virus-like particles were also produced for the first time for PEDv, and were able to form with just the membrane …
Effects Of Prenatal Exposure To Bisphenol A On Fetal Lung Development, Ayten Hijazi
Effects Of Prenatal Exposure To Bisphenol A On Fetal Lung Development, Ayten Hijazi
Electronic Thesis and Dissertation Repository
Prenatal exposure to bisphenol A (BPA), one of the most prevalent endocrine disrupting chemicals, is associated with lung dysfunction and diseases in later life. However, it is unknown if this association has a fetal origin. In this thesis, a series of in vivo and in vitro experiments were conducted to determine the effects of prenatal exposure to BPA on fetal lung development, and define the underlying molecular mechanisms. Environmentally relevant doses of BPA were administered to pregnant mice via diet from embryonic day (E) 7.5 to 18.5. Fetal lungs were analyzed at E18.5 for changes in structure and expression of …