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Full-Text Articles in Medicine and Health Sciences
Vacht Overexpression Increases Acetylcholine At The Synaptic Cleft And Accelerates Aging Of Neuromuscular Junctions, Satoshi Sugita, Leland L. Fleming, Caleb Wood, Sydney K. Vaughan, Matheus P. S. M. Gomes, Wallace Camargo, Ligia A. Naves, Vania F. Prado, Marco A. M. Prado, Cristina Guatimosim, Gregorio Valdez
Vacht Overexpression Increases Acetylcholine At The Synaptic Cleft And Accelerates Aging Of Neuromuscular Junctions, Satoshi Sugita, Leland L. Fleming, Caleb Wood, Sydney K. Vaughan, Matheus P. S. M. Gomes, Wallace Camargo, Ligia A. Naves, Vania F. Prado, Marco A. M. Prado, Cristina Guatimosim, Gregorio Valdez
Anatomy and Cell Biology Publications
Background: Cholinergic dysfunction occurs during aging and in a variety of diseases, including amyotrophic lateral sclerosis (ALS). However, it remains unknown whether changes in cholinergic transmission contributes to age-and disease-related degeneration of the motor system. Here we investigated the effect of moderately increasing levels of synaptic acetylcholine (ACh) on the neuromuscular junction (NMJ), muscle fibers, and motor neurons during development and aging and in a mouse model for amyotrophic lateral sclerosis (ALS). Methods: Chat-ChR2-EYFP (VAChTHyp) mice containing multiple copies of the vesicular acetylcholine transporter (VAChT), mutant superoxide dismutase 1 (SOD1G93A), and Chat-IRES-Cre and tdTomato transgenic mice were used in this …
Hydrogen Peroxide Induced Loss Of Heterozygosity Correlates With Replicative Lifespan And Mitotic Asymmetry In Saccharomyces Cerevisiae, Emine Guven, Lindsay A. Parnell, Erin D. Jackson, Meighan Parker, Nilin Gupta, Jenny Rodrigues, Hong Qin
Hydrogen Peroxide Induced Loss Of Heterozygosity Correlates With Replicative Lifespan And Mitotic Asymmetry In Saccharomyces Cerevisiae, Emine Guven, Lindsay A. Parnell, Erin D. Jackson, Meighan Parker, Nilin Gupta, Jenny Rodrigues, Hong Qin
Scholarly Works
Cellular aging in Saccharomyces cerevisiae can lead to genomic instability and impaired mitotic asymmetry. To investigate the role of oxidative stress in cellular aging, we examined the effect of exogenous hydrogen peroxide on genomic instability and mitotic asymmetry in a collection of yeast strains with diverse backgrounds. We treated yeast cells with hydrogen peroxide and monitored the changes of viability and the frequencies of loss of heterozygosity (LOH) in response to hydrogen peroxide doses. The mid-transition points of viability and LOH were quantified using sigmoid mathematical functions. We found that the increase of hydrogen peroxide dependent genomic instability often occurs …