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Articles 1 - 3 of 3
Full-Text Articles in Medicine and Health Sciences
Cloning, Protein Expression, And Characterization Of Interleukin 1 Alpha, Musaab Habeeb Ali Al Ameer
Cloning, Protein Expression, And Characterization Of Interleukin 1 Alpha, Musaab Habeeb Ali Al Ameer
Graduate Theses and Dissertations
Recombinant DNA technology and the ability to produce recombinant proteins have significantly changed the world of pharmaceutical market. Recombinant DNA technology using E. coli cells has successfully produced many therapeutic proteins. In this study, the designed tag Ark-RUBY-tag facilitates rapid, and reproducible purification of recombinant proteins expressed as inclusion bodies in E. coli cells. Purification of Ark-RUBY-fused recombinant protein(s) can be obtained by using imidazole fractions. Target protein can be easily removed from the Ark-RUBY-tag by enzymatic cleavage. Ark-RUBY-fused recombinant proteins can be quantitatively detected using polyclonal antibodies. Ark-RUBY-tag can be used to purify small peptides. By using Ark-RUBY-tag, purified …
Structural And Functional Characterization Of Mitochondria With Trna Mutations, Brooke Henry
Structural And Functional Characterization Of Mitochondria With Trna Mutations, Brooke Henry
Biological Sciences Undergraduate Honors Theses
Mitochondrial encephalopathy, lactic acidosis, and stroke-like episodes (MELAS) is one of the most common disorders associated with mitochondrial tRNA mutations. One of the most common causes of MELAS is mutation in the MT-TL1 gene which codes for mitochondrial tRNA Leucine (UUR). Mutation in MT-TE gene, another mitochondrial gene which encodes for mitochondrial tRNA Glutamate (GAA/G), has been implicated in various mitochondrial related myopathies. It remains unclear how point mutations in these tRNA genes result in disease onset and progression. Here, we report an early comparative analysis of fibroblast cell lines derived from patients carrying two different tRNA mutations: m.3243A>G …
Distal Urogenital Anatomy Of Male Prairie Racerunners, Aspidoscelis Sexlineatus Viridis (Reptilia: Sauria: Teiidae), Stanley E. Trauth
Distal Urogenital Anatomy Of Male Prairie Racerunners, Aspidoscelis Sexlineatus Viridis (Reptilia: Sauria: Teiidae), Stanley E. Trauth
Journal of the Arkansas Academy of Science
I examined histologically the distal urogenital anatomy in male Prairie Racerunners (Aspidoscelis sexlineatus viridis) from a small seasonal sample of individuals collected in Arkansas in order to provide additional information regarding squamate urogenital anatomy. Specifically, I focused on the basic anatomy and positioning of posterior ducts and associated structures in this teiid lizard. The anatomical structures included the ductus deferens, ampulla ductus deferens, ampulla urogenital papilla (Aup), ureter, inner core tissue mass, urodaeum, and the urogenital papilla. The two Aup, which are small complimentary blind pouches representing the terminal repositories for products released by urogenital ducts, are striking …