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Articles 1 - 5 of 5
Full-Text Articles in Medicine and Health Sciences
The Effects Of Sex On Zebrafish Bone Metabolism, Simon Bagatto
The Effects Of Sex On Zebrafish Bone Metabolism, Simon Bagatto
Williams Honors College, Honors Research Projects
I investigated the effects of diet and sex on zebrafish bone metabolism. Zebrafish were subjected to either a high-calorie or low-calorie diet over a five-week period. After this diet, zebrafish scales were used to measure alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP) enzyme activity with a fluorescent substrate. The enzyme activities were markers of the osteoblasts (ALP) and the osteoclasts (TRAP). These data were compared among sex and diet of the zebrafish using a repeated measures ANOVA statistical test. I also measured the number of resorption lacunae per scale (another indicator of osteoclast activity). The results showed higher TRAP …
Knockout Of Endospanin 1 Via Crispr In Zebrafish, Danio Rerio, Jared Kittinger
Knockout Of Endospanin 1 Via Crispr In Zebrafish, Danio Rerio, Jared Kittinger
Williams Honors College, Honors Research Projects
I made endospanin 1 knockout (KO) zebrafish to examine its effects on lipid and bone metabolism. Endospanin 1, or leptin receptor overlapping transcript (leprot), is a cytosolic protein linked to the protein hormone leptin that influences the trafficking of leptin receptors to the plasma membrane of cells. Genes for endospanin and tyrosinase (a pigmentation enzyme) were targeted via a microinjection of guide RNAs and CRISPR Cas9 into zebrafish embryos at 2-4 cell stages. I was able to disrupt the endospanin 1 gene (based upon the disruption of tyrosinase), but very few mutant zebrafish fully developed into adults. Only low KO …
Neural Stem Cell Metabolic Changes In The Presence Of Interferon-Gamma, Dylan Mckibben
Neural Stem Cell Metabolic Changes In The Presence Of Interferon-Gamma, Dylan Mckibben
Williams Honors College, Honors Research Projects
Spinal cord injury results in significant disability and health care costs due to an inability of the mature central nervous system to regenerate. One potential therapy would be implantation of neural stem cells to the injury site and stimulation of their differentiation to mature neurons. Previous work has shown that the cytokine interferon-gamma can promote this process; however, the signaling pathways induced in neural stem cells have not been well-defined. This proposal examines neural stem cell metabolic responses to signaling induced by interferon-gamma. Two questions will be answered: i) What specific metabolic pathway is activated to guide neural stem cell …
Leucine Supplementation In Cuprizone-Induced Oligodendrocyte Toxicity, Michael Ley
Leucine Supplementation In Cuprizone-Induced Oligodendrocyte Toxicity, Michael Ley
Williams Honors College, Honors Research Projects
Cuprizone is a copper chelator that induces demyelination in the central nervous system when fed to mice. This compound is thought to target Complex IV and disrupt mitochondrial metabolism leading to loss of myelin-producing oligodendrocytes. In this proposal, we further examine the chemical and toxicological properties of cuprizone. Immunofluorescence imaging and microarray data on MO3.13 cells treated with cuprizone revealed the possibility of alterations in lysosomal function, as well as mitochondrial disruption via mTOR and Electron Transport Chain (ETC) related pathways. Cell viability assays conducted suggest that addition of Branched-Chain Amino Acids, most commonly used from literature, leucine, can increase …
Body Composition Vs Athletic Performance In Volleyball, Elizabeth Decaro
Body Composition Vs Athletic Performance In Volleyball, Elizabeth Decaro
Williams Honors College, Honors Research Projects
Objectives: The goal of this study was to determine if body composition affected athletic performance in the sport of volleyball at the University of Akron.
Methods: The study was conducted in the School of Sports Science & Wellness Education on the 3rd floor of Infocision Stadium at the University of Akron using the computer containing the Bod Pod data for the women’s volleyball team.
Results: 20 subjects of the women’s varsity volleyball team were studied. The averages of each test were varied between the different skills tests in counter-movement vertical jump, approach vertical jump, block, broad jump, chin-ups, and …