Medicine and Health Sciences Commons^™

Comparison Of Milk Microbiota Between Healthy And Mastitic Cows, Sibel Kizil, Fatma Esin Aydin, Cansu Önlen Güneri̇, Ufuk Ülker, Gürol Emekdaş, İbrahim Basmaci, Serkan Erat, Rıza Durmaz, Efsun Melike Çeçen, Asya Kazan

Turkish Journal of Veterinary & Animal Sciences

Mammary gland infections occur due to bacterial changes in the mammary tissue. Studies conducted in recent years have reported variations in the most common bacteria differ according to geographical locations. California mastitis test (CMT), somatic cell count (SCC), and aerobic colony count (ACC) analyses were performed on approximately 50 mL of hygienically collected raw milk samples. Raw milk was also subjected to conventional bacteriological isolation and identification. Bacterial diversity and rates in raw milk were compared through metagenome analysis. Two samples, one from healthy milk and another from subclinical milk with mastitis, were independently tested to determine whether there were …

Determination Of Virulence Genes And Antibiotic Resistance Profiles Of Streptococcus Agalactiae Isolated From Buffalo Milk, Nevi̇n Tuzcu, Hasan Hüseyi̇n Hadi̇mli̇, Beatri̇z Padron

Turkish Journal of Veterinary & Animal Sciences

Mastitis is a contagious disease that causes significant milk loss and serious economic damage in dairy animals. Streptococcus agalactiae causes clinical and subclinical mastitis in animals and infections in humans, especially adults, and newborns. This study aimed to confirm the identification of S. agalactiae isolates by PCR in buffalo milk; to determine the antibiotic resistance and virulence genes by multiplex PCR. The material of this study consisted of 30 (12%) S. agalactiae strains identified by phenotypic, biochemical, and PCR methods (rRNA-16S) from 250 isolates with bacterial growth from milk samples of buffaloes with mastitis. In addition, the Kirby-Bauer disc diffusion …

Rumen-Protected Methionine Supplementation Improves Lactation, Oxidative Status, And Immune Response During A Subclinical Mastitis Challenge In Lactating Dairy Cows., Anita Paz Lopez

Electronic Theses and Dissertations

Mastitis is a significant problem in the dairy industry, posing numerous challenges for dairy farmers worldwide. Mastitis refers to the inflammation of the mammary gland, primarily caused by bacterial infections. This condition affects the health and well-being of dairy cows and has severe economic implications for farmers and overall dairy production. The prevalence of mastitis substantially threatens milk quality, productivity, and profitability, making it a top concern in the dairy industry. Preventing mastitis in cows is crucial for maintaining the herd's health and ensuring highquality milk production. Nutritional strategies have recently gained greater attention due to a direct impact on …

Investigations On Some Aerobic Bacterial Agents Isolated From Heifers At The Beginning Ofthe First Lactation, Tuncay Çökülgen, Uçkun Sai̇t Uçan

Turkish Journal of Veterinary & Animal Sciences

In this study, it was aimed to identify the predominant coagulase-negative staphylococci (CNS) species in subclinical, clinical, and persistent infections in Konya province, to determine various virulence and resistance mechanisms of CNS species within the agelactation level. The research has been carried out in a dairy herd that is free from brucellosis and tuberculosis and uses an automated herd management system. Thirty-two heifers at the beginning of lactation with milk yields of more than 20 liters have been observed in terms of udder infections during the first 1-4 months of lactation. The prevalence of intramammary infections (IMI) and of CNS-caused …

A Comparative Study Of Blood And Milk Chemiluminescence In Healthy Cows And Withsubclinical And Clinical Mastitis, Radoslaw Skorzewski, Wieslaw Krumrych, Hanna Markiewicz, Elzbieta Sandurska

Turkish Journal of Veterinary & Animal Sciences

The aim of the study was to evaluate the luminol-dependent spontaneous and stimulated chemiluminescence (CL) of whole blood and milk in cows with subclinical and clinical mastitis. The research was carried out on 108 Holstein-Friesian dairy cows aged 3-5 years. Based on the assessment of somatic cell count (SCC) in quarter milk, the animals were divided into 3 groups: I - clinically healthy cows (n = 35), II - animals with subclinical mastitis (n = 30) and III - cows with clinical mastitis (n = 43). The material for the research was whole blood and quarter milk. The samples were …

Application Of An Indirect Mila Elisa For The Detection Of Mycoplasma Bovis Antibodiesin Bovine Milk, Abd Al Bar Al Farha, Nadeeka Wawegama, Farhid Hemmatzadeh, Simon Firestone, Jamie Moffat, Gholam Ali Kojouri, Ania Ahaniazari, Reza Amanollahi, Andrew Hoare, Kiro Petrovski

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to detect Mycoplasma bovis specific antibodies using a recently reported MilA ELISA with the aim to detect M. bovis antibodies in milk. An indirect ELISA, based on a recombinant fragment of the Mycoplasma immunogenic lipase A (MilA) protein, was conducted on 291 milk samples for the detection of M. bovis antibodies. Samples were also tested with conventional Mycoplasma culture and M. bovis PCR. Samples were collected from individual cows from 2 commercial dairy herds in South Australia. Of 291 samples tested, 68 (23.4%) were detected positive for M. bovis antibodies, 150 (51.5%) were positive …

Evaluation Of Casein Hydrolysate As An Alternative Dry-Off Treatment And Milk Quality Management Tool In Dairy Cows, Justine Elena Britten

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Mastitis, an infection of the mammary gland, is the most common and expensive animal health problem for the dairy industry and affects every dairy farm to some degree. This disease complex is painful for dairy cows, increases the on-farm use of antibiotics, presents a threat to milk quality and is a waste of time, money and milk production. Each year, the dairy industry loses as much as a billion dollars to mastitis.

Many cows will experience mastitis at least once during a lactation cycle and some animals will develop recurring mastitis episodes in a single mammary quarter. These mastitic quarters …

Identification Of Polymorphism In The Bovine Interleukin-17a Gene And Its Association With Mastitis In Polish Holstein?Friesian Cattle, Magdalena Dusza, Joanna Pokorska, Dominika Ku?Aj, Joanna Makulska

Turkish Journal of Veterinary & Animal Sciences

The main aim of this study was to identify the association between the single nucleotide polymorphism (SNP) in the bovine interleukin-17A (IL-17A) gene and mastitis resistance. Additionally, the connection between the identified polymorphism and the somatic cell score (SCS) was tested. Two hundred and six Polish Holstein?Friesian cows were genotyped using the PCR-RFLP method. One SNP mutation (c.126G>A) was identified within the coding sequence of the analyzed gene. Results of logistic regression analysis displayed no significant association between the IL-17A genotype and clinical mastitis occurrence (Wald chi-square test = 0.74; P = 0.69). The GLM analysis demonstrated that genotype …

Identification Of Candida Strains With Nested Pcr In Bovinemastitis And Determination Of Antifungal Susceptibilities, Göksel Erbaş, Uğur Parin, Şükrü Kirkan, Serap Savaşan, Volkan Özavci, Hafi̇ze Tuğba Yüksel

Turkish Journal of Veterinary & Animal Sciences

Mastitis is one of the biggest problems of dairy cattle industries. In recent years, fungal agents have been frequently reported among the causative agents for mastitis. Among the fungal agents, Candida species are the most common. This study's aims were the isolation, identification, and determination of antifungal susceptibility of Candida species causing mycotic mastitis in cattle. A total of 260 mastitic milk samples were collected from different farms. Identification was conducted by rapid diagnostic tests and nested PCR method; 6 different antifungal agents were examined. Candida sp. was detected in 46 (17.7%) of the 260 mastitic milk samples. Based on …

Identifying Genetic Factors In Streptococcus Uberis That Enable Evasion Of The Host Immune Response, Alexis Christine Burnham

Chancellor’s Honors Program Projects

No abstract provided.

Evaluation Of Immunotherapeutic Potential Of Ocimum Sanctumin Bovine Subclinical Mastitis, Tawheed Ahmad Shafi, Baljinder Kumar Bansal, Dhiraj Kumar Gupta, Shashi Nayyar

Turkish Journal of Veterinary & Animal Sciences

Mastitis, the inflammation of mammary glands, is caused by a wide range of contagious and environmental microbes, whose clearance from the udder is generally dependent on the use of antibiotics. However, antibiotic therapy may result in development of drug-resistant microbes and depress the activity of polymorphonuclear cells. The present study was therefore undertaken to explore the immunotherapeutic potential of an herb, Ocimum sanctum, in mastitis in dairy cows. The study involved twenty HF × Sahiwal lactating dairy cows, each identified with at least one specific subclinical mastitis quarter as per International Dairy Federation criteria. The cows were divided into two …

Herd-Specific Autovaccine And Antibiotic Treatment In Elimination Of Staphylococcus Aureus Mastitis In Dairy Cattle, Danuta Czernomysy-Furowicz, Karol Fijalkowski, Anna Silecka, Jolanta Karakulska, Pawel Nawrotek, Radoslaw Drozd, Magdalena Ferlas, Jacek Borkowski, Dorota Jankowiak

Turkish Journal of Veterinary & Animal Sciences

The aim of the present study was to compare the effectiveness of cefuroxime, herd-specific autovaccine, and cefuroxime/herd-specific autovaccine treatments in eliminating S. aureus from the milk of 45 cows with subclinical mastitis. Prior to this study, cows with S. aureus mastitis were divided into 3 groups of 15 cows each. Group 1 received cefuroxime, group 2 received the autovaccine, and group 3 received cefuroxime and the autovaccine. The antibiotic was infused into the teat canal, whereas the autovaccine was administrated in the upper udder lymph node region. It was revealed that after day 35 of the treatment S. aureus was …

An Investigation Of Epigenetic Contributions To Inter-Animal And Age Dependent Variation In The Bovine Innate Immune Response., Benjamin Green

Graduate College Dissertations and Theses

Mastitis represents a major issue within the dairy industry responsible for economic loss via decreased animal productivity and associated veterinary costs. Currently, there is a push to identify a phenotypic innate immune response that will yield dairy cows with an enhanced resistance to mastitis. Bovine dermal fibroblasts were used as a cell model to measure the response of individuals to Gram-negative bacterial stimuli through the TLR4 signaling pathway. Fibroblast cultures were isolated from 15 dairy heifers at 5, 11, and 16 months of age in order to determine the variability in responsiveness to LPS as well as to monitor the …

Determination Of Plasma Fibrinogen And Haptoglobin, Hematological And Blood Biochemical Changes In Bulgarian Local Goats With Experimentally Induced Staphylococcus Aureus Mastitis, Ivan Fasulkov, Manol Karadaev, Nasko Vasilev, Valentina Urumova, Teodora Mircheva

Turkish Journal of Veterinary & Animal Sciences

The aim of the present study was to assess changes in acute phase proteins fibrinogen and haptoglobin, as well as hematological, blood biochemical, and microbiological alterations in local Bulgarian goats with experimentally induced Staphylococcus aureus mastitis. The experiments were conducted with 6 clinically healthy local Bulgarian goats, 2 to 6 years of age, weighing 46-57 kg. The experimental infection was done with a stationary phase culture of a field Staphylococcus aureus strain isolated from a cow with clinical mastitis. Blood haptoglobin in goats was considerably higher (P < 0.05) as early as 8 h after the pathogen's inoculation. The most significant differences from baseline values occurred 24 and 48 h (P < 0.01) after infection. Plasma fibrinogen increased significantly (P < 0.05) 8 h after infection and reached the highest mean concentration (9.12 g/L) by 72 h. Leukocytosis was established as early as 4 h after the experimental infection when leukocyte counts were significantly higher than baseline (P < 0.001). The analysis of results indicates that in experimentally induced Staphylococcus aureus mastitis, significantly higher (P < 0.05) levels of the leukocyte, fibrinogen, and haptoglobin are established as early as 8 h after the infection.

Investigation Of The Efficacy Of Tarantula Cubensis Extract (Theranekron D6) In The Treatment Of Subclinical And Clinical Mastitis In Dairy Cows, Kutlay Gürbulak, Aytaç Akçay, Kadi̇r Semi̇h Gümüşsoy, Birgit Sist, Simone Steiner, Murat Abay, Esra Canooğlu, Tayfur Bekyürek

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to investigate the effects of the use of Theranekron alone or in combination with intramammary antibiotic therapy for the treatment of subclinical and clinical mastitis on the recovery rate from mastitis. The study material was composed of 177 mammary lobes of 63 Holstein cows with subclinical (groups S1 and S2) and clinical mastitis (groups C1, C2, C3, and C4). In all of the treatment groups, excluding group S2, the California mastitis test scores were determined to have decreased after treatment (P < 0.05). In groups C1, C2, C3, and C4, which were treated for clinical mastitis, the somatic cell count (SCC) values were observed to have decreased. The decrease in SCC values was statistically significant except in group C2 (P < 0.01). While the SCC values were observed to have decreased in group S1 (P > 0.05), which was treated with Theranekron alone, the same values were found to …

Susceptibility Of Prototheca Zopfii Strains Isolated From Cows With Mastitis To Chlorhexidine And Iodine, Henryk Krukowski, Andrzej Lisowski, Bozena Nowakowicz-Debek, Lukasz Wlazlo

Turkish Journal of Veterinary & Animal Sciences

Bovine mastitis caused by Prototheca spp. can assume high significance because of economic losses and the potential risk to public health. The aim of our study was to evaluate the effect of chlorhexidine and iodine on Prototheca zopfii strains isolated from bovine milk. For this study, 15 Prototheca zopfii isolates previously obtained from cows with clinical and subclinical mastitis in Poland were used. The in vitro susceptibility tests to chlorhexidine and iodine of the strains of Prototheca zopfii for the determination of their minimal microbicidal concentrations (MMCs) were performed using the tube dilution method in Sabouraud dextrose broth and the …

Characterisation Of Coagulase Positive Staphylococcus Species Isolated From Bovine Mastitis Using Protein And Plasmid Patterns, Emi̇ne Arslan, Ayten Çelebi̇, Leyla Açik, Uçkun Sai̇t Uçan

Turkish Journal of Veterinary & Animal Sciences

The objective of this work was to study the protein patterns, plasmid profiles, and antibiotic susceptibility of Staphylococcus intermedius and Staphylococcus aureus isolates originating from mastitic mammary glands of dairy cattle in different parts of Konya province. A total of 114 Staphylococcus species were isolated and identified by conventional bacteriological methods from bovine mastitis. Of the total isolates 77 were identified as S. aureus and 37 as S. intermedius. Intra- and inter-species diversities in the coagulase-positive staphylococci were investigated by analysis of whole-cell protein profiles using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Plasmid profiling also demonstrated that 75 S. aureus …

Investigation Of The Incidence And Diagnosis Of Subclinical Mastitis In Early Lactation Period Cows, Ayhan Baştan, Ci̇han Kaçar, Duygu B. Acar, Mi̇that Şahi̇n, Mehmet Cengi̇z

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to compare the results of the California mastitis test (CMT) and bacteriological culture for detection of subclinical intramammary infections after 10 days postpartum. Samples were collected from 102 cows. The CMT was performed once on each cow. The results of bacteriological culture and the CMT were compared in 344 milk samples. Two hundred fifty samples were CMT (+) and 94 samples were CMT (-); 224 samples were bacteriologic culture (+) and 120 samples were culture (-). Both CMT and culture (+) samples numbered 212, CMT and culture (-) samples 82, CMT (+) and culture …

Disseminated Streptococcus Equi Subsp. Zooepidemicus Infection In A Foal And Associated Mastitis In A Mare, A. Levent Kocabiyik, Esra Büyükcangaz, Ahmet Akkoç, Cüneyt Özakin, İ. Taci̇ Cangül

Turkish Journal of Veterinary & Animal Sciences

A systemic infection with Streptococcus equi subsp. zooepidemicus is described in a 22-day-old female foal for the first time in Turkey. Clinically the foal was in poor condition and had severe pneumonia. Painful swellings of the carpal and tarsal joints were evident. Examination of the dam revealed clinical mastitis. Postmortem histopathological and microbiological examinations of samples from the foal revealed a disseminated infection with S. equi subsp. zooepidemicus. The causative agent was also recovered in pure culture from a milk sample of the mare.

A Comparison Of Pyronin Y-Methyl Green Stain And Methylene Blue Stain For Somatic Cell Count In Sheep Milk, Emily Mirek, Stacey O’Donnell

Senior Honors Projects

Somatic cell count is a key method used to evaluate the quality of milk in today’s growing dairy sheep industry. Somatic cells are body cells, primarily the white blood cells, found in a milk sample. If an infection such as mastitis is present, the number of somatic cells in the milk increases (Gonzalo, et al. 1992). Producers routinely perform somatic cell counts on cow and goat milk. For dairy cattle in the United States, the somatic cell count can not exceed 750,000 cells per milliliter, for commercial milk. For dairy goats the accepted limit is 1,000,000 cells per mL of …

Coagulase Gene Polymorphism Of Staphylococcus Aureus Isolated From Subclinical Bovine Mastitis, Özkan Aslantaş, Cemi̇l Demi̇r, Hülya Türütoğlu, Zafer Canteki̇n, Yaşar Ergün, Gökhan Doğruer

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to investigate the coagulase gene polymorphism of Staphylococcus aureus isolated from subclinical bovine mastitic milk samples from 3 different Turkish cities (Hatay, Gaziantep, and Burdur). Amplification of the coagulase gene from 80 S. aureus isolates produced 5 different polymerase chain reaction (PCR) products; 79 isolates showed only 1 amplicon, and 1 isolate showed 2 amplicons. The isolates were grouped into 9 genotypes by analysing the restriction fragment length polymorphism (RFLP) of the gene; the 2 most common genotypes accounted for 73.8% of the isolates. The results indicated that many coagulase gene genotypes are present in the …

Plasmids And Protein Patterns Of Escherichia Coli Isolated From Bovine Mastitis In Konya, Turkey, Uçkun Sai̇t Uçan, Leyla Açik, Ayten Çelebi̇, Osman Ergani̇ş, Emi̇ne Arslan

Turkish Journal of Veterinary & Animal Sciences

In this study, a total of 30 Escherichia coli isolates obtained from milk samples of dairy cows suffering from subclinical mastitis in Konya, Turkey were typed according to plasmids and protein patterns. Agarose gel electrophoresis and sodium doedecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) methods were used to identify plasmids and whole-cell protein profiles. Of these two methods, SDS-PAGE typing proved to be more discriminate for typing the isolates.

Identification And Antimicrobial Susceptibility Of Staphylococcus Aureus And Coagulase Negative Staphylococci From Bovine Mastitis In The Aydın Region Of Turkey, Şükrü Kirkan, Ergün Ö. Göksoy, Osman Kaya

Turkish Journal of Veterinary & Animal Sciences

Three hundred bovine milk samples with mastitis were used. Eighty-five (28.33%) strains were identified as Staphylococcus aureus whereas 60 (20.00%) strains were isolated as coagulase negative staphylococcus. Bacteriological cultures of coagulase negative staphylococcus were identified as 20 (33.33%) strains of S. hyicus, 16 (26.66%) strains of S. chromogenes, 9 (15.00%) strains of S. epidermidis, 5 (8.33%) strains of S. haemolyticus, 4 (6.66%) strains of S. sciuri, 3 (5.00%) strains of S. lentis and 3 (5.00%) strains of S. cohnii subsp. cohnii. The antibiotic susceptibility test results showed that Staphylococcus aureus strains were susceptible to ciprofloxacin (100.00%), neomycin + bacitracin + …

Bacteriological Investigation Of Udder Lobes Of Cows With Mastitis Slaughtered In The Elazığ Region, Hasan Basri̇ Gülcü, Hasan Basri̇ Ertaş

Turkish Journal of Veterinary & Animal Sciences

Bacteriological examinations were performed on 206 mammary tissue samples taken from 160 cows with mastitis in Elazığ. The isolates were identified as Staphylococcus aureus (39.04%), Staphylococcus epidermidis (17.81%), Actinomyces pyogenes (14.38%), Escherichia coli (8.9%), Streptococcus agalactiae (6.16%), Streptococcus uberis (4.11%), Streptococcus dysgalactiae (3.42%), Corynebacterium bovis (1.37%), Pasteurella haemolytica (1.37%) and Bacillus cereus (1.37%).

Cost Of Mastitis In Scottish Dairy Herds With Low And High Subclinical Mastitis Problems, Cengi̇z Yalçin

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to estimate the cost of mastitis and the contribution of each cost component of mastitis to the total mastitis induced cost in herds with low and high levels of subclinical mastitis under Scottish field conditions. It was estimated that mastitis cost £140 per cow/year to the average Scottish dairy farmer in 1996. However, this figure was as low as £69 per cow/year in herds with lower levels of subclinical mastitis, and as high as £228 cow/year in herds with high sub- clinical mastitis. The magnitude and proportion of the cost components in the total …

Effects Of Mastitis On Milk Yield And Composition, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

EVERY DAIRY farmer knows how much he spends on the treatment of cows affected with mastitis, but the actual loss in production is more difficult to estimate.

As part of the current mastitis research programme the Department of Agriculture's Animal and Dairying Divisions surveyed 37 dairy herds with a believed high incidence of mastitis.

Can Mastitis Be Eliminated?, F C. Wilkinson

Journal of the Department of Agriculture, Western Australia, Series 4

Can mastitis be eliminated from a dairy herd? The results of a 12-month continuous study of four commercial herds show that it could be done.

Mastitis In Western Australian Dairy Cattle, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

IN 1964/65, as a first step towards a State-wide mastitis control programme, the Department of Agriculture undertook a survey of mastitis in West Australian dairy herds.

The survey was designed to obtain a clear picture of the incidence and severity of the disease and is the most comprehensive mastitis survey carried out in Australia.

Mastitis : Modified Teat Cups Give Better Back-Flushing, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

Back-flushing of the teat cups has not brought the expected reduction in the spread of mastitis in some dairy herds.

Replacement of the conventional nut-and-tail nipple with a special flushing nipple, or use of a one-piece teat cup liner, will ensure that flushing is efficient.

Animal Husbandry Research At Bramley, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

A progress report of animal husbandry investigations at the Bramley Animal Husbandry Research Station, Margaret River.

BRAMLEY RESEARCH STATION is four miles north of Margaret River.

The average annual rainfall of 45 inches falls mainly in the winter, providing a seven-month growing season which starts in March or April.

The June-July period is excessively wet, causing extensive waterlogging of the soils.