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Full-Text Articles in Medicine and Health Sciences
Virological And Pathological Investigation Of Canine Parvovirus-2 (Cpv-2) With The Assessment Of The Genetic Variability Of Field Strains, Zeynep Karapinar, Musa Karaman, İhsan Kisadere, Mustafa Usta, Mehmet Özkan Ti̇murkan
Virological And Pathological Investigation Of Canine Parvovirus-2 (Cpv-2) With The Assessment Of The Genetic Variability Of Field Strains, Zeynep Karapinar, Musa Karaman, İhsan Kisadere, Mustafa Usta, Mehmet Özkan Ti̇murkan
Turkish Journal of Veterinary & Animal Sciences
Canine parvovirus-2 (CPV-2), which is common worldwide, is a highly contagious pathogen in domestic dogs and some wild carnivore species. The agent causes a multisystemic infection in affected animals, especially acute hemorrhagic enteritis and fatal myocarditis in puppies. The study aimed to reveal the presence of virus in dogs with clinical symptoms of CPV-2 infection using virological, pathological, and immunohistochemical methods and make molecular characterization based on partial VP-2 gene of strains circulating in the region. As a result of PCR studies, 9 out of 16 rectal swab samples and 17 out of 38 blood samples were positive. A phylogenetic …
Distribution Of The Misfolded Isoform Of The Prion Protein In Peripheral Tissues And Spinal Cord Of Rocky Mountain Elk (Cervus Elaphus Nelsoni) With Naturally Occurring Chronic Wasting Disease, Terry R. Spraker, Thomas Gidlewski, J. G. Powers, T. A. Nichols, M. A. Wild
Distribution Of The Misfolded Isoform Of The Prion Protein In Peripheral Tissues And Spinal Cord Of Rocky Mountain Elk (Cervus Elaphus Nelsoni) With Naturally Occurring Chronic Wasting Disease, Terry R. Spraker, Thomas Gidlewski, J. G. Powers, T. A. Nichols, M. A. Wild
USDA Wildlife Services: Staff Publications
Chronic wasting disease (CWD) is an infectious transmissible spongiform encephalopathy of cervids associated with the presence of a misfolded prion protein (PrPCWD). Progression of PrPCWD distribution has been described using immunohistochemistry and histologic changes in a single section of brain stem at the level of the obex resulting in scores from 0 (early) to 10 (terminal) in elk with naturally occurring CWD. Here we describe the spread and distribution of PrPCWD in peripheral tissues and spinal cord in 16 wild and 17 farmed Rocky Mountain elk (Cervus elaphus nelsoni) with naturally occurring CWD and correlate these findings with obex scores. …
Examination Of Canine Herpesvirus-1 In Dogs By Serological, Molecular, And Pathological Methods, Serkan Yildirim, Fatma Gülten Bayraktar, Hakan Aydin, Yavuz Seli̇m Sağlam, Gi̇zem Eser, Muhammet Bahaeddi̇n Dörtbudak
Examination Of Canine Herpesvirus-1 In Dogs By Serological, Molecular, And Pathological Methods, Serkan Yildirim, Fatma Gülten Bayraktar, Hakan Aydin, Yavuz Seli̇m Sağlam, Gi̇zem Eser, Muhammet Bahaeddi̇n Dörtbudak
Turkish Journal of Veterinary & Animal Sciences
In this study, it was aimed to examine Canine Herpesvirus-1 (CHV-1) with different diagnostic methods and investigate the pathological changes in positive samples. For this purpose, 100 dog uteruses of different ages and breeds, were removed by ovariohysterectomy and their blood samples were used as the study material. Enzyme-linked immunosorbent assay (ELISA) and Indirect Fluorescent Antibody Test (IFAT) for serological diagnosis, polymerase chain reaction (PCR) method for molecular diagnosis, immunohistochemistry (IHC) and immunofluorescence (IF) staining for immunopathological diagnosis were performed on the samples. Additionally, hematoxylin and eosin (HE) staining was performed to examine the histopathological changes in the CHV- 1-positive …
A Nasopharyngeal Inflammatory Polyp In A Cat: Histopathology, Immunohistochemistry, And Ct Scan Findings, Hamid Reza Moosavian, Hamideh Esmaeilzadeh, Ramesh Vahabi, Rana Vafaei, Mohammad Reza Esmailinejad
A Nasopharyngeal Inflammatory Polyp In A Cat: Histopathology, Immunohistochemistry, And Ct Scan Findings, Hamid Reza Moosavian, Hamideh Esmaeilzadeh, Ramesh Vahabi, Rana Vafaei, Mohammad Reza Esmailinejad
Turkish Journal of Veterinary & Animal Sciences
The etiology of feline nasopharyngeal inflammatory polyps (FNIPs) has not been completely understood. To the best of our knowledge, there is no study having evaluated the immunohistochemical features of FNIPs. More studies about the histopathological characteristics of such lesions can play a pivotal role in further understanding the disease. We present the clinical manifestations, computed tomography (CT) scan, histopathology, and immunohistochemistry features of a FNIP in a 7-year-old male neutered domestic shorthair cat. CT scan examination showed nasopharyngeal space occupation with some parts of destruction in nasal cavity. Immunohistochemical study of the sections demonstrated the presence of CD3$^{+}$ lymphocytes, and …
Ovarian Stimulation Induces High Expression Of Interleukin-1ss And Disrupts The Histological Features Of The Fallopian Tube, Mahmoud Moussa, Sameh Mohamed Farouk
Ovarian Stimulation Induces High Expression Of Interleukin-1ss And Disrupts The Histological Features Of The Fallopian Tube, Mahmoud Moussa, Sameh Mohamed Farouk
Turkish Journal of Veterinary & Animal Sciences
The objective of this study was to investigate whether there is an association between ovarian stimulation and interrupted gamete interaction in the rat fallopian tube in vivo. We evaluated the protein expression of interleukin-1ß (IL-1ß) via immunohistochemistry and assessed the key histological features of the ovaries, fallopian tubes, and uteri of the rats following treatment with mild (15 IU) and high (30 IU) doses of gonadotropins compared to an unstimulated control group. The serum concentration of estradiol was also determined by immunoassay. The results revealed that high levels of circulating estrogen were associated with higher expression of the IL-1ß protein …
Immunohistochemical Expression Of Secretory Proteins In Pneumonic Rat Lungs, Enver Beytut
Immunohistochemical Expression Of Secretory Proteins In Pneumonic Rat Lungs, Enver Beytut
Turkish Journal of Veterinary & Animal Sciences
The present study evaluated the expression of secretory proteins involving surfactant proteins (SP-A, SP-B, proSP-C), Clara cell secretory protein (CCSP), and thyroid transcription factor-I (TTF-I), together with lambda light chain IgG (λ-IgG), proliferating cell nuclear antigen (PCNA), and lymphocytic phenotypes (T and B cells) in the pneumonic rat lungs. The most prominent gross lesion was severe pulmonary abscession. The lungs showed severe parenchymal destruction and necrosis encapsulated by fibrous tissue. Immunostaining for SPs displayed evident hyperplasia of type II pneumocytes. Immunoreaction to SP-A and SP-B occurred strongly on the luminal surface of type II cells, and to proSP-C in the …
Pathological And Immunohistochemical Evaluation Of Skin And Teat Papillomas In Cattle, Enver Beytut
Pathological And Immunohistochemical Evaluation Of Skin And Teat Papillomas In Cattle, Enver Beytut
Turkish Journal of Veterinary & Animal Sciences
Bovine skin and teat papillomas were examined for immunohistochemical expression of bovine papillomavirus (BPV), proliferating antigens (PCNA, Ki-67), CD3+ T lymphocytes, and some of the type III intermediate filaments. Papillomas showed epidermal hyperplasia, koilocytes, and hyperkeratosis with prominent dermal connective tissue. Immunohistochemistry found the presence of BPV antigens in the nuclei of granulosa cells. PCNA and Ki-67 revealed positive nuclear staining in the dermis and epidermis. Perivascular cuffing in the dermis predominantly consisted of CD3+ T cells. Immunoreaction to vimentin was strong in the connective tissue of the dermis. $\alpha$SMA positivity was mainly in the media of vessels. Smooth muscle …
Extraskeletal Chondroblastic Osteosarcoma Of The Subcutaneous Tissue In A Maltese Dog, Byungjoon Seung, Junghyung Ju, Seunghee Cho, Soohyeon Kim, Hwan Choi, Junghyang Sur
Extraskeletal Chondroblastic Osteosarcoma Of The Subcutaneous Tissue In A Maltese Dog, Byungjoon Seung, Junghyung Ju, Seunghee Cho, Soohyeon Kim, Hwan Choi, Junghyang Sur
Turkish Journal of Veterinary & Animal Sciences
A 10-year-old spayed female Maltese dog presented to a local animal hospital with a subcutaneous mass (4 x 3 x 3 cm) in the right shoulder region. The mass was well circumscribed, with soft tissue opacity and variable levels of mineralization, but with no bony involvement in radiography. The mass was surgically removed. Upon histological examination, the mass consisted of malignant mesenchymal cells that had produced a chondroid matrix and osteoid. Round to polygonal neoplastic cells with large nuclei showed moderate anisokaryosis and variable numbers of mitotic figures. The tumor cells were positive for vimentin and osteocalcin and negative for …
Immunohistochemical Expression Of Surfactant Apoproteins In Pneumonic Ovine Lungs, Enver Beytut
Immunohistochemical Expression Of Surfactant Apoproteins In Pneumonic Ovine Lungs, Enver Beytut
Turkish Journal of Veterinary & Animal Sciences
The present study was undertaken to investigate the immunohistochemical expression of pulmonary surfactant proteins (SP-A, SP-B, proSP-C) associated with proliferating cell nuclear antigen (PCNA), thyroid transcription factor-I (TTF-I), and lymphocytic phenotypes (CD3+ T and CD79αcy+ B cells) in ovine lungs with exudative and verminous pneumonia. Pneumonic lungs (n = 50) were classified as presenting fibrinous bronchopneumonia (n = 16), abscessing necrotic bronchopneumonia (n = 10), verminous pneumonia (n = 15), and interstitial pneumonia (n = 9). The lungs with fibrinous bronchopneumonia displayed consolidation of lobes with adhesion to surrounding tissues. The lungs with verminous pneumonia were characterized by lungworm sections …
Trophoblast Major Histocompatibility Complex Class I Expression Is Associated With Immune-Mediated Rejection Of Bovine Fetuses Produced By Cloning, Heloisa M. Rutigliano, Aaron J. Thomas, Amanda Wilhelm, Benjamin R. Sessions, Brady A. Hicks, Donald H. Schlafer, Kenneth L. White, Christopher J. Davies
Trophoblast Major Histocompatibility Complex Class I Expression Is Associated With Immune-Mediated Rejection Of Bovine Fetuses Produced By Cloning, Heloisa M. Rutigliano, Aaron J. Thomas, Amanda Wilhelm, Benjamin R. Sessions, Brady A. Hicks, Donald H. Schlafer, Kenneth L. White, Christopher J. Davies
Animal, Dairy, and Veterinary Science Faculty Publications
Trophoblast cells from bovine somatic cell nuclear transfer (SCNT) conceptuses express major histocompatibility complex class I (MHC-I) proteins early in gestation, and this may be one cause of the significant first-trimester embryonic mortality observed in these pregnancies. MHC-I homozygous-compatible (n = 9), homozygous-incompatible (n = 8), and heterozygous-incompatible (n = 5) SCNT pregnancies were established. The control group consisted of eight pregnancies produced by artificial insemination. Uterine and placental samples were collected on Day 35 ± 1 of pregnancy, and expression of MHC-I, leukocyte markers, and cytokines were examined by immunohistochemistry. Trophoblast cells from all SCNT pregnancies expressed MHC-I, while …
Mast Cell Typing In Aortic Onchocerciasis And The Immunohistochemicaldemonstration Of Wolbachia Bacteria, Rahşan Yilmaz, Melek Koçak, Zafer Özyildiz, İsmai̇l Şah Harem
Mast Cell Typing In Aortic Onchocerciasis And The Immunohistochemicaldemonstration Of Wolbachia Bacteria, Rahşan Yilmaz, Melek Koçak, Zafer Özyildiz, İsmai̇l Şah Harem
Turkish Journal of Veterinary & Animal Sciences
In this study, the presence of endosymbiotic Wolbachia bacteria was determined by immunohistochemical methods in 8 out of 43 paraffin-embedded bovine aortic tissues that were macroscopically and microscopically diagnosed with aortic onchocerciasis in the archive of the Harran University Faculty of Veterinary Medicine's Department of Pathology. By means of the enzyme histochemical and immunohistochemical staining methods, it was ascertained that the tryptase-containing subtype of mast cells (MCT) was involved in the inflammatory response in the bovine aortae with onchocerciasis. The aim of this study was to elucidate the pathogenesis of aortic onchocerciasis lesions caused by Onchocerca armillata in Turkey and …
Immunohistochemical Evaluation Of Canine And Felinemerkel Cell Tumors?A Report Of Two Cases, Mateusz Mikiewicz, Iwona Otrocka-Domagala, Katarzyna Pazdzior-Czapula, Michal Gesek, Anna Mikolajczyk, Agnieszka Blacha
Immunohistochemical Evaluation Of Canine And Felinemerkel Cell Tumors?A Report Of Two Cases, Mateusz Mikiewicz, Iwona Otrocka-Domagala, Katarzyna Pazdzior-Czapula, Michal Gesek, Anna Mikolajczyk, Agnieszka Blacha
Turkish Journal of Veterinary & Animal Sciences
Merkel cell tumors, a type of cutaneous round-cell tumors, are rarely observed in dogs and cats. This tumor type originates from cutaneous neuroendocrine cells. In the present study, the morphology of canine and feline Merkel cell tumors and their immunoexpression of neuron-specific enolase, cytokeratin, chromogranin A, Ki67, and metallothionein were evaluated using histopathological and immunohistochemical methods. The canine Merkel cell tumor displayed a diffuse morphological pattern, whereas the feline tumor displayed a solid morphological pattern. Both tumors were positive for neuron-specific enolase and cytokeratin, but negative for chromogranin A. The Ki67 indexes in the canine and feline tumors were 7% …
The Effects Of Vincristine Sulfate On Expression Of Galectin-3, Bcl-2, And Carbohydrate Structures Specific For Eel, Gsl-1, And Rca-1 Lectins In Bitches With Naturally Occurring Canine Transmissible Venereal Tumor, Musa Özgür Özyi̇ği̇t, Deni̇z Nak, Hasan Akşi̇t, Sevda İnan Öztürkoğlu, Gözde Şi̇mşek, Ender Uzabaci, Yavuz Nak, Kami̇l Seyrek
The Effects Of Vincristine Sulfate On Expression Of Galectin-3, Bcl-2, And Carbohydrate Structures Specific For Eel, Gsl-1, And Rca-1 Lectins In Bitches With Naturally Occurring Canine Transmissible Venereal Tumor, Musa Özgür Özyi̇ği̇t, Deni̇z Nak, Hasan Akşi̇t, Sevda İnan Öztürkoğlu, Gözde Şi̇mşek, Ender Uzabaci, Yavuz Nak, Kami̇l Seyrek
Turkish Journal of Veterinary & Animal Sciences
Vincristine sulfate is one of the most effective chemical agents used in canine transmissible venereal tumor (CTVT) chemotherapy. Its therapeutic effectiveness is pronounced and significant at the beginning of treatment. The present study was designed to investigate vincristine sulfate treatment and the relationship between CTVT tumor regression and apoptosis [estimated by TdT-mediated dUTP-biotin nick end labeling (TUNEL)], galectin-3 (H-160), Bcl-2 (N-9), and lectin [EEL (B-135), GSL I (L-1100), and RCA I (BA-0084)] expression using immunohistochemical staining in 20 bitches with naturally occurring CTVTs. Evaluation was semiquantitatively performed by HSCORE values using light microscopy. All observed staining was intracytoplasmic and increased …
A Disseminated Cutaneous Plasmacytoma Case In A Dog, Burçak Özkan, Si̇nem Ülgen, Funda Yildirim, Ali̇ Riza Kiziler, Kürşat Özer, Mehmet Erman Or, Aydin Gürel, Ümi̇t Bora Barutçu, Hazi̇m Tamer Dodurka
A Disseminated Cutaneous Plasmacytoma Case In A Dog, Burçak Özkan, Si̇nem Ülgen, Funda Yildirim, Ali̇ Riza Kiziler, Kürşat Özer, Mehmet Erman Or, Aydin Gürel, Ümi̇t Bora Barutçu, Hazi̇m Tamer Dodurka
Turkish Journal of Veterinary & Animal Sciences
A 5-year-old mixed-breed dog presenting with diffuse lesions was diagnosed with cutaneous plasmacytoma. Sites where tumors occurred most often involved the skin of the facial areas, as well as that of the shoulder, neck, and back of the body. No systemic findings were present in spite of a significant enlargement of the lesions. The tumors varied in diameter, the largest being 5-10 cm. Tissue samples obtained from tumoral masses were fixed in 10% formalin, processed routinely, embedded in paraffin, cut into sections of 4-5 µm, and stained with hematoxylin and eosin. To determine the nature of the tumoral cells, kappa …
Immunohistochemical Distribution Of Glutathione Peroxidase And Its Gene Expression Via Rt-Pcr In The Liver Tissue Of Melatonin-Administered Mice, Serap Koral Taşçi, Nurhayat Gülmez
Immunohistochemical Distribution Of Glutathione Peroxidase And Its Gene Expression Via Rt-Pcr In The Liver Tissue Of Melatonin-Administered Mice, Serap Koral Taşçi, Nurhayat Gülmez
Turkish Journal of Veterinary & Animal Sciences
The purpose of this study is to investigate the effect of exogenous administration of melatonin, which is a strong antioxidant agent, on the gene expression of glutathione peroxidase, which is one of the antioxidant enzymes, in the livers of mice and also to examine the immunohistochemical localization of the enzyme. The 24 male Swiss albino mice used in the study were divided into 3 groups: experimental (n = 8), sham (n = 8), and control (n = 8). A 10 mg/kg dose of melatonin was administered intraperitoneally to the experimental group for 4 weeks. Only ethanol and a serum physiological …
Immunohistochemical Localization Of Transforming Growth Factor Beta 2 And Gene Expression Using Real-Time Pcr In Capsaicin-Administered Rat Testis During Puberty, Gökhan Nur, Mümtaz Nazli, Sevda Eli̇ş Yildiz
Immunohistochemical Localization Of Transforming Growth Factor Beta 2 And Gene Expression Using Real-Time Pcr In Capsaicin-Administered Rat Testis During Puberty, Gökhan Nur, Mümtaz Nazli, Sevda Eli̇ş Yildiz
Turkish Journal of Veterinary & Animal Sciences
The objective of this study was to investigate the effects of capsaicin on the testis distribution of transforming growth factor beta 2 (TGF\beta2) and gene expression using real-time PCR in pubescent rats. In this study, rats were divided into 3 groups: capsaicin (CAP), sham, and control. The CAP group was injected with 1 mg/kg doses of capsaicin (10% ethanol and 1% Tween in an 80% distilled water mixture) subcutaneously every day for a period of 1 week. The sham group was injected with a solution of 10% ethanol and 1% Tween in a mixture of 80% distilled water and nothing …
Immunohistochemical Distribution Of Glucagon -, Insulin -, Somatostatin -, Gastrin-,And Serotonin-Containing Cells In The Pancreas Of The Van Cat, Turan Karaca, Adem Kara, Nejdet Şi̇mşek, Sema Uslu, Deni̇z Teki̇ner, Meci̇t Yörük
Immunohistochemical Distribution Of Glucagon -, Insulin -, Somatostatin -, Gastrin-,And Serotonin-Containing Cells In The Pancreas Of The Van Cat, Turan Karaca, Adem Kara, Nejdet Şi̇mşek, Sema Uslu, Deni̇z Teki̇ner, Meci̇t Yörük
Turkish Journal of Veterinary & Animal Sciences
The regional distribution, relative frequency, and appearance of glucagon (A-cell)-, insulin (B-cell)-, somatostatin (D-cell)-, gastrin (G-cell)-, and serotonin (EC-cell)-secreting cells in the endocrine and exocrine pancreas of Van cats were examined using the immunohistochemistry method. Glucagon immunopositive A-cells were principally found in the central region of the islets of Langerhans, while insulin immunopositive B-cells were located in the periphery of the pancreatic islets. Moreover, several A- and B-cells were observed as only single cells or clusters of 2 to 3 immunopositive cells in the exocrine parenchyma and the pancreatic duct epithelium. Somatostatin and gastrin immunopositive reactivities were negligible in the …
Immunohistochemical Localization Of Glutathione Peroxidase 1 Enzyme And Its Gene Expression By Rt-Pcr In The Liver Tissue Of Healthy And Diabetic Mice, Turgay Deprem, Nurhayat Gülmez
Immunohistochemical Localization Of Glutathione Peroxidase 1 Enzyme And Its Gene Expression By Rt-Pcr In The Liver Tissue Of Healthy And Diabetic Mice, Turgay Deprem, Nurhayat Gülmez
Turkish Journal of Veterinary & Animal Sciences
The purpose of this study is to examine the gene expression level of glutathione peroxidase 1 (GPx1) in the liver of healthy and diabetic mice, its localization in the tissue, and structural changes in the liver. In this study, 36 Swiss albino mice were divided into 3 groups: experimental (diabetic) (n = 15), sham (n = 15), and control (n = 6). Streptozotocin (100 mg/kg) was intraperitoneally administered to mice in the experimental group. While the gene expression level of GPx1 was determined using the RT-PCR method, its localization in the liver was determined using an immunohistochemical method. The GPx1 …
An Immunohistochemical Study On The Distribution Of Endocrine Cells In The Digestive Tract Of Gray Goose (Anser Anser), Jun Yang, Lei Zhang, Xin Li, Leii Zhang, Xiangjiang Liu, Kemei Peng
An Immunohistochemical Study On The Distribution Of Endocrine Cells In The Digestive Tract Of Gray Goose (Anser Anser), Jun Yang, Lei Zhang, Xin Li, Leii Zhang, Xiangjiang Liu, Kemei Peng
Turkish Journal of Veterinary & Animal Sciences
The objective of this study was to investigate the morphology and the distribution of 5-hydroxytryptamine (5-HT), somatostatin (SS), gastrin (Gas), glucagon (Glu), and substance P immunoreactive (IR) cells in the digestive tract of gray goose by the immunohistochemical streptavidin-peroxidase method. The samples were taken from 10 healthy adult gray geese. The results showed that 5 kinds of IR cells were mainly distributed between the mucous epithelium and intestinal gland. The number of 5-HT-IR cells was highest in the rectum and duodenum, but none were observed in the pylorus. SS-IR cells appeared in great numbers in the pylorus, duodenum, and cecum; …
Malignant Giant-Cell Tumor Of Bone With Lymph Node Involvement In A Cat, Ahmet Akkoç, Gürsel Sönmez, Kemal Yanik, Ayli̇n Alasonyalilar Demi̇rer, Ni̇hal Y. Gül, M. Carmen Ferreras-Estrada
Malignant Giant-Cell Tumor Of Bone With Lymph Node Involvement In A Cat, Ahmet Akkoç, Gürsel Sönmez, Kemal Yanik, Ayli̇n Alasonyalilar Demi̇rer, Ni̇hal Y. Gül, M. Carmen Ferreras-Estrada
Turkish Journal of Veterinary & Animal Sciences
The present study describes giant-cell tumor of bone (GCToB) with lymph node involvement in a 5-year-old crossbred cat. The animal was referred to the surgery clinic with progressive subcutaneous swelling in the left proximal femoral region, severe lameness, constipation, and dysuria. A moderately firm, subcutaneous, palpable mass, 9 cm in diameter, was observed, and biopsy samples were taken. Histopathologically, the mass was constituted by ovoid-shaped mononuclear cells intermixed with many multinucleated giant cells (MGC). Immunohistochemically, the giant cells were positively stained with antivimentin, and the same cells were negative for antidesmin and anti-S100 staining. Tartrate-resistant acid phosphatase (TRAP) activity in …
Pathological And Immunohistochemical Evaluation Of The Effects Of Interferon Gamma (Ifn-Γ) And Aminoguanidine In Rats Experimentally Infected With Fasciola Hepatica, Enver Beytut, Ati̇la Akça, Hali̇l İbrahi̇m Gökce
Pathological And Immunohistochemical Evaluation Of The Effects Of Interferon Gamma (Ifn-Γ) And Aminoguanidine In Rats Experimentally Infected With Fasciola Hepatica, Enver Beytut, Ati̇la Akça, Hali̇l İbrahi̇m Gökce
Turkish Journal of Veterinary & Animal Sciences
The present study was aimed at pathologically and immunohistochemically evaluating the effects of interferon gamma (IFN-\gamma) and aminoguanidine (AG) in rats experimentally infected with Fasciola hepatica. A total of 44 Wistar rats were divided into 4 groups. With the exception of the controls (fourth group), the remaining rats were infected orally with 25 metacerceria of F. hepatica. The first group was administered 0.2% AG in drinking water daily, in order to block nitric oxide (NO) production. The second group was administered 250 units of IFN-\gamma daily, in order to stimulate NO synthesis. The third group was administered a placebo only. …
Primary Vaginal Fibroleiomyosarcoma In A 4-Year-Old Holstein-Friesian Cow, Hamdi̇ Avci, Güneş Seri̇n, Ahmet Aydoğan, Serap Bi̇ri̇nci̇oğlu
Primary Vaginal Fibroleiomyosarcoma In A 4-Year-Old Holstein-Friesian Cow, Hamdi̇ Avci, Güneş Seri̇n, Ahmet Aydoğan, Serap Bi̇ri̇nci̇oğlu
Turkish Journal of Veterinary & Animal Sciences
A case of fibroleiomyosarcoma of the vagina in a 4-year-old Holstein-Friesian cow is described. The tumour was 6.5 × 5 × 4.2 cm in size, weighed 87.73 g, and had a solid appearance. Histologically, the tumour was composed of smooth muscle and fibrous tissue components with spindle-shaped cells with eosinophilic fibrillary cytoplasm. Immunohistochemically, the tumour cells reacted with antibodies to \alpha-SMA, desmin, and vimentin but not with antibodies to CD34, CD68, and S-100 protein. Both histologically and immunohistochemically it showed the features of a fibroleiomyosarcoma.
Evaluation Of Cardiac Troponin I And Inducible Nitric Oxide Synthase Expressions In Lambs With White Muscle Disease, Recai̇ Tunca, Hi̇dayet Meti̇n Erdoğan, Mahmut Sözmen, Mehmet Çi̇ti̇l, Alpaslan Kadi̇r Devri̇m, Serpi̇l Ergi̇nsoy, Erdoğan Uzlu
Evaluation Of Cardiac Troponin I And Inducible Nitric Oxide Synthase Expressions In Lambs With White Muscle Disease, Recai̇ Tunca, Hi̇dayet Meti̇n Erdoğan, Mahmut Sözmen, Mehmet Çi̇ti̇l, Alpaslan Kadi̇r Devri̇m, Serpi̇l Ergi̇nsoy, Erdoğan Uzlu
Turkish Journal of Veterinary & Animal Sciences
White muscle disease (WMD) is a world-wide nutritional disease of all animal species characterised by degeneration of skeletal and cardiac muscles. In the present study we determined the cardiac troponin I (cTnI) and inducible nitric oxide synthase (iNOS) expression in hearts of the lambs with WMD (n = 15). Eight clinically healthy lambs served as control. Creatine kinase (CK), creatine kinase MB (CK-MB), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) activities were analysed for both groups. cTnI levels were measured by a commercially available ELISA kit. Mean cTnI (10.49 ± 0.25 ng/ml), CK (261.16 ± 38.61 U/l), CK-MB (214.16 ± …
Immunohistochemical Studies On S100 Reactivity In Chicks Experimentally Infected With Eimeria Tenella I: The Localization Of S100 Protein And Its Subunits \Alpha And \Beta In Stages Of Eimeria Tenella, Lati̇fe Beyaz, Ayhan Atasever, Feyzullah Beyaz, Anil İça
Immunohistochemical Studies On S100 Reactivity In Chicks Experimentally Infected With Eimeria Tenella I: The Localization Of S100 Protein And Its Subunits \Alpha And \Beta In Stages Of Eimeria Tenella, Lati̇fe Beyaz, Ayhan Atasever, Feyzullah Beyaz, Anil İça
Turkish Journal of Veterinary & Animal Sciences
The expression of the calcium-binding protein S100 and its subunits \alpha and \beta in the intracellular protozoon Eimeria tenella was investigated. For this purpose, anti-S100 whole molecule polyclonal antibody and anti-S100 \alpha and anti-S100 \beta monoclonal antibodies were used on paraffin sections collected from the cecum, ileum, colon, rectum, and bursa of Fabricius of chicks experimentally infected with E. tenella. One hundred seventy-five 2- to 3-day-old specific pathogen-free chicks were used. The chicks were divided into experiment and control groups, to include 94 and 81 chicks, respectively. All the chicks in the experimental group received 10,000 spored oocytes in 0.5 …
Pathological, Immunohistochemical, And Bacteriological Findings In The Mammary Glands And Supramammary Lymph Nodes Of Cows With A History Of Abortion Due To Brucella Abortus, Enver Beytut, Mi̇that Şahi̇n, Serpi̇l Ergi̇nsoy, Mahmut Sözmen
Pathological, Immunohistochemical, And Bacteriological Findings In The Mammary Glands And Supramammary Lymph Nodes Of Cows With A History Of Abortion Due To Brucella Abortus, Enver Beytut, Mi̇that Şahi̇n, Serpi̇l Ergi̇nsoy, Mahmut Sözmen
Turkish Journal of Veterinary & Animal Sciences
The present study examined the mammary glands and supramammary lymph nodes of cows with a history of abortion due to Brucella abortus, and presents the histopathological, immunohistochemical, and bacteriological results. The study included 11 Brucella-seropositive cows that aborted during their first gestation. Histopathologically, the mammary glands showed lymphoplasmacytic and histiocytic interstitial mastitis. The lymph nodes had lymphofollicular hyperplasia and medullary plasmacytosis. Immunohistochemistry results showed brucellar antigens, predominantly in the cytoplasm of macrophages, and neutrophils in the intralobular interstitium and periductal stroma in the udders of 3 cows. Desquamated alveolar epithelium also indicated intense immunopositivity. In the lymph nodes, macrophages containing …
Multicentric Intrahepatic Biliary Cystadenoma In A Dog, Ahmet Akkoç, İ. Taci̇ Cangül, M. Özgür Özyi̇ği̇t
Multicentric Intrahepatic Biliary Cystadenoma In A Dog, Ahmet Akkoç, İ. Taci̇ Cangül, M. Özgür Özyi̇ği̇t
Turkish Journal of Veterinary & Animal Sciences
This report describes a case of multicentric intrahepatic biliary cystadenoma observed in a 13-year-old, male, Irish setter dog. The dog had a history of chronic inappetence, weight loss, intermittent icterus, and diarrhoea. In the ultrasonographic examination of the abdominal organs, 5 spherical masses were found in various liver lobes. The largest mass was 12 cm in diameter and was located on the quadrate lobe. It had spongy consistency and had several cystic structures, 3 mm-2.5 cm in diameter, on the cut surface. While this mass bulged through the normal outline of the liver, smaller masses were engraved within the liver …
Ileal Leiomyoma In A Captive Zebra (Equus Burchelli), Rahşan Yilmaz, Ahmet Akkoç, M. Özgür Özyi̇ği̇t
Ileal Leiomyoma In A Captive Zebra (Equus Burchelli), Rahşan Yilmaz, Ahmet Akkoç, M. Özgür Özyi̇ği̇t
Turkish Journal of Veterinary & Animal Sciences
In the present study, an ileal leiomyoma encountered in a captive zebra (Equus burchelli) was reported. A 29-year-old, female zebra was found dead in the Bursa zoo. According to the referring veterinarian the most prominent clinical signs were intermittent colic (responsive to analgesics) and constipation. At post mortem examination, abdomen was severely distended. A firm, multilobular, well vascularized and encapsulated tumoural mass, 12 cm in diameter, embedded into the proximal part of the ileum wall, and protruded from the serosal surface was found. The cut section of the mass had whorled appearance, pale yellow to white in colour, and had …
Surfactant Proteins A And D In The Seminal Plasma Of Stallions, Ayhan Ata, Orhan Kankavi̇, M.Aki̇f Çi̇ftçi̇oğlu
Surfactant Proteins A And D In The Seminal Plasma Of Stallions, Ayhan Ata, Orhan Kankavi̇, M.Aki̇f Çi̇ftçi̇oğlu
Turkish Journal of Veterinary & Animal Sciences
We have previously shown that surfactant protein (SP)-A and SP-D are present in the stallion´s genital system. The aim of the present study was to evaluate the expression and presence of SP-A and SP-D in the seminal plasma and accessory sex glands of stallions. We characterized further the SP-A and SP-D in the seminal plasma of stallions by western blotting and immunohistochemically in accessory sex glands. On one-dimensional SDS-PAGE electrophoresis and western blots, bands for SP-A (34 kDa) and SP-D (43 kDa) in seminal plasma and accessory sex gland tissue extracts were detected. The similarity between biochemical properties (relative molecular …
Pathological, Immunohistochemical, And Electron Microscopic Findings In The Respiratory Tract And Skin Of Chickens Naturally Infected With Avipoxvirus, Enver Beytut, Mehmet Haligur
Pathological, Immunohistochemical, And Electron Microscopic Findings In The Respiratory Tract And Skin Of Chickens Naturally Infected With Avipoxvirus, Enver Beytut, Mehmet Haligur
Turkish Journal of Veterinary & Animal Sciences
The present study describes pathological, immunohistochemical, and electron microscopic findings in the respiratory tract and skin of chickens naturally infected with avian pox virus. Respiratory distress, depression, and cyanosis were found in the chickens upon clinical examination. At necropsy, erosion, crusts, and nodules were observed on the comb, wattle, and eyelids. Histopathological examination revealed that the epithelial cells in the stratum spinosum were hyperplastic, and that their cytoplasm contained large, eosinophilic inclusion bodies. Epithelial hyperplasia was also prominent in the mucosa of the trachea and bronchi, with characteristic cytoplasmic inclusion bodies and severe submucosal accumulation of mononuclear cells. Avipoxvirus infection …
Immunohistochemical Expression Of Estrogen And Progesterone Receptors In Oviduct Adenocarcinomas In Laying Hens, M. Özgür Özyi̇ği̇t, Gürsel Sönmez
Immunohistochemical Expression Of Estrogen And Progesterone Receptors In Oviduct Adenocarcinomas In Laying Hens, M. Özgür Özyi̇ği̇t, Gürsel Sönmez
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to demonstrate the presence of estrogen and progesterone receptors (ERs and PRs, respectively) in oviduct adenocarcinomas in laying hens. In all, 32 oviduct adenocarcinomas (18 primary tumors and 14 intestinal metastases) from Babcock B-380 laying hens 16-18 months old were stained with commercially available monoclonal antibodies against mouse-anti-human ERs and mouse-anti-human PRs. In the microscopic examination, 13 tumors (7 oviduct adenocarcinomas and 6 intestinal metastases) stained positively for ERs and 12 tumors (4 oviduct adenocarcinomas and 8 intestinal metastases) gave a positive reaction to PR antibody. The staining pattern of the tumor cells with …