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Full-Text Articles in Medicine and Health Sciences
Detection, Cloning, And Sequencing Of The Enterotoxin Gene Of Clostridium Perfringens Type C Isolated From Goat, Yuan Tang, Ming Wen, Bin Lian, Jianzhong Cheng, Kaigong Wang, Bijun Zhou
Detection, Cloning, And Sequencing Of The Enterotoxin Gene Of Clostridium Perfringens Type C Isolated From Goat, Yuan Tang, Ming Wen, Bin Lian, Jianzhong Cheng, Kaigong Wang, Bijun Zhou
Turkish Journal of Veterinary & Animal Sciences
Clostridium perfringens enterotoxin (CPE) is an important virulence factor involved in C. perfringens food poisoning and other gastrointestinal illnesses. In our experiment a Clostridium perfringens enterotoxin gene (cpe) positive type C strain was obtained by multiplex PCR from 34 C. perfringens isolates from goat in Guizhou Province, China. Then a pair of specific primers was designed and synthesized according to the published cpe gene, and the complete cpe gene was amplified from the genomic DNA of the cpe-positive C. perfringens isolate. The PCR product was cloned into pMD18-T vectors and transformed into competent cell DH5a. The recombinant plasmid was identified …
Prolonged Dystocia, Uterine Necrosis, And Ovariohysterectomy In A Chios Ewe, Christos N. Brozos, Lazaros Lazarides, Isidoros Karagiannis, Evangelos Kiossis, Georgios Tsousis, Vasilios Psychas, Nektarios Giadinis
Prolonged Dystocia, Uterine Necrosis, And Ovariohysterectomy In A Chios Ewe, Christos N. Brozos, Lazaros Lazarides, Isidoros Karagiannis, Evangelos Kiossis, Georgios Tsousis, Vasilios Psychas, Nektarios Giadinis
Turkish Journal of Veterinary & Animal Sciences
A 4-year-old Chios ewe was submitted to the Clinic of Farm Animals (School of Veterinary Medicine, Aristotle University of Thessaloniki). The animal had delivered 2 healthy lambs, 2 days earlier, and was inappetent, depressed, and reluctant to move, while it had tachycardia and tachypnea. The cervix was opened at a dilation of 1 finger in diameter and fetid fetal membranes expelled from the vagina. Serum biochemistry revealed renal and liver disease, while ultrasonography revealed uterine dilatation. Laparotomy revealed a partly autolysed embryo in the uterus and gangrenous metritis. Clostridium perfringens and Escherichia coli were isolated in bacterial cultures from the …
Toxinotyping Of Clostridium Perfringens Isolates By Elisa And Pcr From Lambs Suspected Of Enterotoxemia, Hasan Hüseyi̇n Hadi̇mli̇, Osman Ergani̇ş, Zafer Sayin, Zeki̇ Aras
Toxinotyping Of Clostridium Perfringens Isolates By Elisa And Pcr From Lambs Suspected Of Enterotoxemia, Hasan Hüseyi̇n Hadi̇mli̇, Osman Ergani̇ş, Zafer Sayin, Zeki̇ Aras
Turkish Journal of Veterinary & Animal Sciences
Clostridium perfringens is a gram-positive, anaerobic bacterium that causes a wide range of diseases in humans and animals. The purposes of this study were to determine, using the enzyme-linked immunosorbent assay (ELISA), the clostridial toxins in intestinal samples of lambs with suspected enterotoxemia; to conduct molecular typing of C. perfringens isolates; and to investigate the presence of the genes of 4 major toxins (\alpha, \beta, \epsilon, and \iota) in the isolates by polymerase chain reaction (PCR). According to the ELISA results, clostridial toxins were determined in 32 intestinal samples. Of the samples, 13 (40.62%) were C. perfringens type A, 1 …