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Using Detection Dogs And Rspf Models To Assess Habitat Suitability For Bears In Greater Yellowstone, Jon P. Beckmann, Lisette P. Waits, Aimee Hurt, Alice Whitelaw, Scott Bergen Dec 2015

Using Detection Dogs And Rspf Models To Assess Habitat Suitability For Bears In Greater Yellowstone, Jon P. Beckmann, Lisette P. Waits, Aimee Hurt, Alice Whitelaw, Scott Bergen

Western North American Naturalist

In the northern U.S. Rockies, including the Greater Yellowstone Ecosystem (GYE), connectivity is a concern because large carnivores have difficulties dispersing successfully between protected areas. One area of high conservation value because of its importance for connecting the GYE to wilderness areas of central Idaho is the Centennial Mountains and surrounding valleys (2500 km2) along the Idaho–Montana border just west of Yellowstone National Park. The current expansion of grizzly bears (Ursus arctos) and other large carnivore populations outside protected areas of Yellowstone National Park and Grand Teton National Park has placed a greater emphasis on potential linkage zones …


Effects Of Potential Detoxifying Agents On Growth Performance And Deoxynivalenol (Don) Urinary Balance Characteristics Of Nursery Pigs Fed Don-Contaminated Wheat, H. L. Frobose, E. W. Stephenson, M. D. Tokach, J. M. Derouchey, S. S. Dritz, R. D. Goodband, J. C. Woodworth, R. E. Musser Jan 2015

Effects Of Potential Detoxifying Agents On Growth Performance And Deoxynivalenol (Don) Urinary Balance Characteristics Of Nursery Pigs Fed Don-Contaminated Wheat, H. L. Frobose, E. W. Stephenson, M. D. Tokach, J. M. Derouchey, S. S. Dritz, R. D. Goodband, J. C. Woodworth, R. E. Musser

Kansas Agricultural Experiment Station Research Reports

Two experiments were conducted to evaluate the effects of detoxifying agents on the growth performance of nursery pigs fed diets contaminated with deoxynivalenol (DON). Naturally DON-contaminated wheat (6 ppm) replaced noncontaminated wheat in diets to achieve desired dietary DON concentrations. Basal ingredients were tested for mycotoxin and amino acid content prior to diet manufacturing. Diets were pelleted at 180˚F with a 45-s conditioning time.

A total of 238 barrows and gilts (PIC 327 × 1050; initially 29.6 ± 5.6 lb and 42 d of age) were used in a 21-d growth study. Pens of pigs were allotted by BW to …


Evaluating The Effects Of Maternal Vitamin D Supplementation On The Subsequent Growth Performance And Carcass Characteristics Of A Subsample Population Of Growing Pigs, J. R. Flohr, J. C. Woodworth, M. D. Tokach, S. S. Dritz, R. D. Goodband, J. M. Derouchey, J. R. Bergstrom Jan 2015

Evaluating The Effects Of Maternal Vitamin D Supplementation On The Subsequent Growth Performance And Carcass Characteristics Of A Subsample Population Of Growing Pigs, J. R. Flohr, J. C. Woodworth, M. D. Tokach, S. S. Dritz, R. D. Goodband, J. M. Derouchey, J. R. Bergstrom

Kansas Agricultural Experiment Station Research Reports

A subsample of 448 growing pigs (PIC 327 × 1050), or approximately 50% of pigs weaned from sows fed varying dietary vitamin D regimens, were used in a split-plot design to determine the influence of maternal and nursery vitamin D regimens on growth performance. Sows were previously administered diets containing vitamin D as either: 1) low vitamin D3(363 IU/lb); 2) medium vitamin D3(907 IU/lb); 3) high vitamin D3(4,354 IU/lb); or 4) 23μg 25(OH)D3/lb (Hy-D, DSM Nutritional Products Inc, Parsippany, NJ) as described by Flohr et al. (20153) throughout gestation and …


Evaluating The Effect Of Manufacturing Porcine Epidemic Diarrhea Virus (Pedv)-Contaminated Feed On Subsequent Feed Mill Environmental Surface Contamination, L. L. Schumacher, R. A. Cochrane, C. E. Evans, J. R. Kalivoda, J. C. Woodworth, C. R. Stark, C. K. Jones, Rodger G. Main, Jianqiang Zhang, S. S. Dritz, Phillip Charles Gauger Jan 2015

Evaluating The Effect Of Manufacturing Porcine Epidemic Diarrhea Virus (Pedv)-Contaminated Feed On Subsequent Feed Mill Environmental Surface Contamination, L. L. Schumacher, R. A. Cochrane, C. E. Evans, J. R. Kalivoda, J. C. Woodworth, C. R. Stark, C. K. Jones, Rodger G. Main, Jianqiang Zhang, S. S. Dritz, Phillip Charles Gauger

Kansas Agricultural Experiment Station Research Reports

This study aimed to utilize the only known pilot feed mill facility approved for pathogenic feed agent use in the United States to evaluate the effect of manufacturing Porcine Epidemic Diarrhea Virus (PEDV)-contaminated feed on subsequent feed mill environmental surface contamination. In this study, PEDV inoculated feed was manufactured and conveyed on equipment along with four subsequent batches of PEDV-free feed. Equipment and environmental surfaces were sampled using swabs and analyzed for the presence of PEDV RNA by PCR. The experiment was replicated three times with decontamination of the feed mill and all equipment between replications. Overall, environmental swabs indicated …


Coccidia (Apicomplexa: Eimeriidae) Of Three-Toed Box Turtles, Terrapene Carolina Triunguis (Reptilia: Testudines), From Arkansas And Oklahoma, C. T. Mcallister, D. Motriuk-Smith, R. S. Seville, C. Hudson, M. B. Connior, H. W. Robison Jan 2015

Coccidia (Apicomplexa: Eimeriidae) Of Three-Toed Box Turtles, Terrapene Carolina Triunguis (Reptilia: Testudines), From Arkansas And Oklahoma, C. T. Mcallister, D. Motriuk-Smith, R. S. Seville, C. Hudson, M. B. Connior, H. W. Robison

Journal of the Arkansas Academy of Science

We collected 50 three-toed box turtles (Terrapene carolina triunguis) from 9 counties of Arkansas and 4 counties of Oklahoma, and examined their feces for coccidial parasites. Nine of 24 (38%) turtles from Arkansas and 8 of 26 (31%) from Oklahoma were found to be passing oocysts of Eimeria ornata. This represents two new geographic distributional records for this coccidian. Measurements of individual isolates of E. ornate as well as morphological characteristics are provided with comparison to its original description and to another Terrapene coccidian, Eimeria carri. In addition, we noted an adelid pseudoparasite being passed by a single T. c. …


Determining The Minimum Infectious Dose Of Porcine Epidemic Diarrhea Virus (Pedv) In A Feed Matrix, L. L. Schumacher, J. C. Woodworth, C. R. Stark, C. K. Jones, R. A. Hesse, Rodger G. Main, Jianqiang Zhang, Phillip Charles Gauger, S. S. Dritz, M. D. Tokach Jan 2015

Determining The Minimum Infectious Dose Of Porcine Epidemic Diarrhea Virus (Pedv) In A Feed Matrix, L. L. Schumacher, J. C. Woodworth, C. R. Stark, C. K. Jones, R. A. Hesse, Rodger G. Main, Jianqiang Zhang, Phillip Charles Gauger, S. S. Dritz, M. D. Tokach

Kansas Agricultural Experiment Station Research Reports

Understanding the magnitude of transmissible risk Porcine Epidemic Diarrhea Virus (PEDV)-infected feed imposes and establishing the minimum infectious dose of PEDV in a feed matrix are important components in strengthening virus prevention and control methods. In this study, an experiment was performed involving 30 crossbred, 10-d-old pigs that were used as a bioassay model for the minimum infectious dose of PEDV in feed. The PEDV was first diluted using tissue culture media to form 8 serial 10-fold dilutions. An aliquot of the original stock virus at 5.6 x 105tissue culture infectious dose/ml (TCID50/ml), each serial PEDV …


Effects Of Commercial Formaldehyde Inclusion And Lysine Level On Pig Performance Of 35- To 50-Lb Nursery Pigs, R. A. Cochrane, L. G. Sica, J. C. Woodworth, S. S. Dritz, C. R. Stark, C. K. Jones Jan 2015

Effects Of Commercial Formaldehyde Inclusion And Lysine Level On Pig Performance Of 35- To 50-Lb Nursery Pigs, R. A. Cochrane, L. G. Sica, J. C. Woodworth, S. S. Dritz, C. R. Stark, C. K. Jones

Kansas Agricultural Experiment Station Research Reports

A total of 299 pigs (DNA 400 × 200; initial BW 33.6 lb) were used in a 14-d study to determine the effects of two separate commercial formaldehyde products (Termin-8; Anitox Corp, Lawrenceville, GA and SalCURB; Kemin Industries, Inc., Des Moines, IA) on nursery pig performance. Dietary treatments were arranged in a 3 × 2 factorial design with three formaldehyde inclusions: none vs. 6.5 lb/ton SalCURB vs. 6.0 lb/ton Termin-8 and 2 Lys levels: Standard (1.25% SID Lys) vs. Low (1.10% SID Lys). Formaldehyde treatments were established based on supplier recommendations and diets were treated with supplier-specific equipment. Pens of …


Effects Of Amino Acid And Energy Intake During Late Gestation On Piglet Birth Weight And Reproductive Performance Of Gilts And Sows Housed Under Commercial Conditions, M. A. Goncalves, K. M. Gourley, S. S. Dritz, M. D. Tokach, N. M. Bello, J. M. Derouchey, J. C. Woodworth, R. D. Goodband Jan 2015

Effects Of Amino Acid And Energy Intake During Late Gestation On Piglet Birth Weight And Reproductive Performance Of Gilts And Sows Housed Under Commercial Conditions, M. A. Goncalves, K. M. Gourley, S. S. Dritz, M. D. Tokach, N. M. Bello, J. M. Derouchey, J. C. Woodworth, R. D. Goodband

Kansas Agricultural Experiment Station Research Reports

The objective of this study was to determine the effects of amino acid (AA) and energy intake during late gestation on piglet birth weight and reproductive performance of high-performing gilts and sows housed under commercial conditions. At d 90 of gestation, a total of 1,102 females (PIC 1050) were housed in pens by parity group (P1 or P2+), blocked by weight within each pen, and each female was randomly assigned to dietary treatments within weight block. Dietary treatments consisted of combinations of 2 standardized ileal digestible (SID) AA (10.7 or 20.0 g SID Lys intake/d with other AA meeting or …


Evaluating Pellet And Meal Feeding Regimens On Finishing Pig Performance, Stomach Morphology, Carcass Characteristics, And Economics, J. A. De Jong, J. M. Derouchey, M. D. Tokach, S. S. Dritz, R. D. Goodband, M. Allerson Jan 2015

Evaluating Pellet And Meal Feeding Regimens On Finishing Pig Performance, Stomach Morphology, Carcass Characteristics, And Economics, J. A. De Jong, J. M. Derouchey, M. D. Tokach, S. S. Dritz, R. D. Goodband, M. Allerson

Kansas Agricultural Experiment Station Research Reports

A total of 2,100 pigs (PIC 327 × 1050, initially 68.8 lb) were used in a 118-d trial to determine the effects of pellet feeding regimens on finishing pig growth performance, stomach morphology, and carcass characteristics. Pens of pigs were balanced by initial BW and randomly allotted to 1 of 6 dietary treatments (14 pens/treatment with 25 pigs/pen). Pens were sorted by gender allowing for 7 barrow and 7 gilt pens/treatment. The same corn-soybean meal–based diets containing 15% dried distillers grains with solubles were used for all treatments and fed in 5 phases. The 6 treatments included a meal or …


Evaluating The Impact Of Maternal Vitamin D Supplementation On Sow Performance, Serum Vitamin Metabolites, Neonatal Muscle And Bone Characteristics, And Subsequent Pre-Weaning Pig Performance, J. R. Flohr, J. C. Woodworth, M. D. Tokach, S. S. Dritz, R. D. Goodband, J. M. Derouchey, J. R. Bergstrom Jan 2015

Evaluating The Impact Of Maternal Vitamin D Supplementation On Sow Performance, Serum Vitamin Metabolites, Neonatal Muscle And Bone Characteristics, And Subsequent Pre-Weaning Pig Performance, J. R. Flohr, J. C. Woodworth, M. D. Tokach, S. S. Dritz, R. D. Goodband, J. M. Derouchey, J. R. Bergstrom

Kansas Agricultural Experiment Station Research Reports

A total of 56 gestating sows (PIC 1050; 35-d post-insemination) were used in a 30-d trial to determine the serum 25(OH)D3response to increasing concentrations of vitamins D3. At initiation, sows were randomly allotted to 1 of 7 dietary vitamin D3treatments (91, 363, 726, 1,451, 2,903, 5,806, or 11,612 IU of vitamin D3/lb of complete diet) with 8 sows per treatment. All sows were fed 5.5 lb daily at 0800. Increasing vitamin D3 increased (quadratic; P<0.001) serum 25(OH)D3with the response depicted by the following prediction equation:

Serum 25(OH)D3,ng/mL = 35.1746 + (0.002353 × …


Effect Of Diet Type And Added Copper On Growth Performance, Carcass Characteristics, Energy Digestibility, Gut Morphology, And Mucosal Mrna Expression Of Finishing Pigs, K. F. Coble, D. D. Burnett, R. D. Goodband, J. M. Gonzales, J. Usry, M. D. Tokach, J. R. Pluske, J. M. Derouchey, J. C. Woodworth, S. S. Dritz, J. R. Flohr, M. A. Vaughn Jan 2015

Effect Of Diet Type And Added Copper On Growth Performance, Carcass Characteristics, Energy Digestibility, Gut Morphology, And Mucosal Mrna Expression Of Finishing Pigs, K. F. Coble, D. D. Burnett, R. D. Goodband, J. M. Gonzales, J. Usry, M. D. Tokach, J. R. Pluske, J. M. Derouchey, J. C. Woodworth, S. S. Dritz, J. R. Flohr, M. A. Vaughn

Kansas Agricultural Experiment Station Research Reports

A total of 757 pigs (PIC 337 × 1050, initially 60.8 lb) were used to determine the effects of added Cu (TBCC, tribasic copper chloride, IntelliBond C; Micronutrients, Inc., Indianapolis, IN) and diet type on growth performance, carcass characteristics, energy digestibility, gut morphology, and mucosal mRNA expression of finishing pigs. Pens of pigs were allotted to 1 of 4 dietary treatments, balanced on average pen weight in a randomized complete-block design with 26 to 28 pigs per pen and 7 replications per treatment. Treatments were arranged as a 2 × 2 factorial with main effects of diet type, a corn-soybean …


Determining The Impact Of By-O-Reg+ In Diets With Or Without A Feed Grade Antibiotic On Growth Performance Of Nursery Pigs, L. L. Thomas, J. C. Woodworth, R. D. Goodband, J. M. Derouchey, M. D. Tokach, S. S. Dritz Jan 2015

Determining The Impact Of By-O-Reg+ In Diets With Or Without A Feed Grade Antibiotic On Growth Performance Of Nursery Pigs, L. L. Thomas, J. C. Woodworth, R. D. Goodband, J. M. Derouchey, M. D. Tokach, S. S. Dritz

Kansas Agricultural Experiment Station Research Reports

A total of 717 nursery pigs (PIC C-29 × 28 and PIC L3-1050 × 1040, initially 12.48 ± 0.10 lb) from two consecutive nursery groups were used in a 35-d growth study. The objective was to determine the impact of increasing levels of By-O-Reg+ in diets with or without 50 g/ton Carbadox. By-O-Reg+ is a unique mixture of essential oils primarily based on oregano. It utilizes encapsulation technology to stabilize the essential oils and is suggested to have antimicrobial-like properties. The present study evaluated growth performance of nursery pigs fed 1 of 6 dietary treatments that were arranged as a …


Frequency Of Hoof Conformation Faults And Disorders In Horses Of Several Breeds, Jaroslaw Luszczynski, Magdalena Pieszka, Anna Durmala, Weronika Pisarczyk, Romana Augustyn, Boguslawa Dlugosz Jan 2015

Frequency Of Hoof Conformation Faults And Disorders In Horses Of Several Breeds, Jaroslaw Luszczynski, Magdalena Pieszka, Anna Durmala, Weronika Pisarczyk, Romana Augustyn, Boguslawa Dlugosz

Turkish Journal of Veterinary & Animal Sciences

The aim of the study was to compare the frequencies of hoof conformation faults and disorders in the front and hind legs of horses of different breeds. The study was conducted on 346 horses over 3 years old (167 Anglo-Arabians, 130 Hucul horses, and 49 Silesian horses). In the study population, over 60% of the horses had some kind of hoof conformation faults and disorders. A significant difference in the incidence of hoof conformation faults (65.5%) and hoof disorders (35.5%) was shown in the analyzed population of horses. In the case of Hucul and Silesian horses, hoof conformation faults were …


Effects Of Probiotics, Oligosaccharides, And Berberine Combinationson Growth Performance Of Pigs, Guoge Fan, Juan Chang, Qingqiang Yin, Xiao Wang, Xiaowei Dang Jan 2015

Effects Of Probiotics, Oligosaccharides, And Berberine Combinationson Growth Performance Of Pigs, Guoge Fan, Juan Chang, Qingqiang Yin, Xiao Wang, Xiaowei Dang

Turkish Journal of Veterinary & Animal Sciences

In order to find alternative feed additives to replace antibiotics for safe animal production, combinations of probiotics, oligosaccharides, and berberine were used in this study. Ninety 60-day-old pigs were assigned to 9 groups, with 10 pigs for each group. Group 1 was the control, group 2 had antibiotics added, and groups 3-9 had different levels of combinations added. The experimental period was 60 days. The results indicated that average daily gain and feed conversion ratio had no significant differences among the 9 groups (P > 0.05); however, the diarrhea rates in the control group and the high probiotic groups with low …


Serum Pharmacokinetics And Tissue Concentrations Of A Newrecrystallized Enrofloxacin Hydrochloride-Dihydrate In Hamsters, Alma Carrascosa, Alejandro De La Peña, Lilia Gutierrez, Hector Sumano Jan 2015

Serum Pharmacokinetics And Tissue Concentrations Of A Newrecrystallized Enrofloxacin Hydrochloride-Dihydrate In Hamsters, Alma Carrascosa, Alejandro De La Peña, Lilia Gutierrez, Hector Sumano

Turkish Journal of Veterinary & Animal Sciences

Pharmacokinetics (PK), pharmacodynamics (PD), and PK/PD ratios of a new solvate of enrofloxacin (enrofloxacin hydrochloride-dihydrate; enro-C), were studied in hamsters. Enrofloxacin from Baytril1397904493 5% (enroR) served as the reference preparation. Two groups of 60 Syrian golden hamsters were intramuscularly injected individually with 10 mg/kg of enroR or with enro-C. Tissue and serum samples were obtained for 72 h; enrofloxacin concentrations were determined by HPLC with UV detection. Ninety percent minimum inhibitory concentrations (MIC90) were determined for strains of methicillin-resistant Staphylococcus aureus, Leptospira interrogans, and Escherichia coli. All PK variables were statistically different between groups (P < 0.01). CMAX of enrofloxacin for enro-C was 17.3 μg/mL and it was 2.6 μg/mL for enroR. AUC was considerably higher for enro-C (459.2 μg/mL h vs. 19.9 μg/mL h). There were no statistically significant differences in MIC90 values between enroR and enro-C. Tissue concentrations of enro-C in all cases were higher and remained above the MIC for longer periods than those of enroR. Relevant PK/PD ratios for enrofloxacin (AUC/MIC ? 125 and CMAX > MIC = 10-12) are consequently …


Vitrification Of In Vitro-Produced Bovine Embryos Matured Inmodified Tcm-199 Medium, Asi̇ye İzem Sandal, Özen Banu Özdaş Jan 2015

Vitrification Of In Vitro-Produced Bovine Embryos Matured Inmodified Tcm-199 Medium, Asi̇ye İzem Sandal, Özen Banu Özdaş

Turkish Journal of Veterinary & Animal Sciences

In vitro-produced bovine embryos matured in modified Tissue Culture Medium 199 (TCM-199) were vitrified at the 7th and 8th days of culture, and development at 48 h after warming was recorded. Ovaries were obtained from a local abattoir, and the oocytes were recovered by slicing method and divided into two main groups. The first group included cysteamine in the TCM-199 (Group A) and the second did not (Group B). They were incubated for 24 h at 38.8 °C in an atmosphere of 5% CO2 in humidified air. Matured oocytes were fertilized with 1-2 × 106/mL of frozen-thawed bull semen. At …


Determination Of The Alui Polymorphism Effect Of Bovine Growth Hormone Gene On Carcass Traits In Zavot Cattle With Analysis Of Covariance, Aytaç Akçay, Bi̇lal Akyüz, Davut Bayram Jan 2015

Determination Of The Alui Polymorphism Effect Of Bovine Growth Hormone Gene On Carcass Traits In Zavot Cattle With Analysis Of Covariance, Aytaç Akçay, Bi̇lal Akyüz, Davut Bayram

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the genotypes of bovine growth hormone and to investigate their association with daily weight gain in the Zavot cattle breed. The bovine growth hormone (GH) genotypes of 45 male Zavot cattle were identified by PCR-RFLP. The effect of the bovine growth hormone gene on daily weight gain was estimated from a dataset obtained from cattle up to the age of 9 months. The mean differences of carcass weight or carcass yield among the genotype groups were assessed by analysis of covariance (ANCOVA). Two alleles for bovine growth hormone locus, L and V, …


Heart Performance Of Lambs And Its Relation To Muscle Volume And Body Surface, Róbert Romvári, Csaba Hancz, Péter Horn, Zsolt Petrási, András Szabó, Dávid Mezöszentgyörgyi, Imre Repa Jan 2015

Heart Performance Of Lambs And Its Relation To Muscle Volume And Body Surface, Róbert Romvári, Csaba Hancz, Péter Horn, Zsolt Petrási, András Szabó, Dávid Mezöszentgyörgyi, Imre Repa

Turkish Journal of Veterinary & Animal Sciences

Electrocardiography-gated dynamic magnetic resonance imaging (MRI) methodology was developed for the in vivo examination of sheep heart characteristics. By combining noninvasive determination of skeletal muscle mass, we studied the relationship between total body skeletal muscle content and heart performance. Measurements were carried out on male Merino lambs using 1.5 T field strength equipment. Average left ventricular volumes were determined and stroke volume was estimated during image postprocessing. Ejection fraction was calculated (73 ± 1.8%) and the cardiac output (CO) value was estimated (2.75 ± 0.16 L/min). After measuring left ventricular wall thickness, contraction values were determined at the septum (62%), …


Functional Properties Of Gelatin Recovered From Scales Of Farmed Sea Bass (Dicentrarchus Labrax), Mehmet Tolga Di̇nçer, Özlem Yeşi̇m Ağçay, Hülya Sargin, Huri̇ye Bayram Jan 2015

Functional Properties Of Gelatin Recovered From Scales Of Farmed Sea Bass (Dicentrarchus Labrax), Mehmet Tolga Di̇nçer, Özlem Yeşi̇m Ağçay, Hülya Sargin, Huri̇ye Bayram

Turkish Journal of Veterinary & Animal Sciences

In the current study gelatin was recovered from scales of farmed sea bass (Dicentrarchus labrax). The first part of the study was planned to determine the yield of acid-soluble collagen and pepsin-soluble collagen from the scales. Both collagens were characterized as type I collagen, containing \alpha-1 and \alpha-2 chains. In the second part, gelatin was extracted using acetic acid and functional properties were determined in this product. Gelatin protein value was found to be 96% and the yield was found to be 18.49%. Proline and hydroxyproline values in the amino acid were found to be high. The gel strength of …


Molecular Characterization Of The Hspa1a Gene By Single-Strandconformation Polymorphism And Sequence Analysis In Holstein-Friesiancrossbred And Deoni Cattle Raised In India, Ramesha P. Kerekoppa, Akhila Rao, Marappa Basavaraju, Gujjar Ramachandra Geetha, Lakhsminarayan Krishnamurthy, Thota Vl Narasimha Rao, Dayal Nitai Das, Kataktalware Mukund Jan 2015

Molecular Characterization Of The Hspa1a Gene By Single-Strandconformation Polymorphism And Sequence Analysis In Holstein-Friesiancrossbred And Deoni Cattle Raised In India, Ramesha P. Kerekoppa, Akhila Rao, Marappa Basavaraju, Gujjar Ramachandra Geetha, Lakhsminarayan Krishnamurthy, Thota Vl Narasimha Rao, Dayal Nitai Das, Kataktalware Mukund

Turkish Journal of Veterinary & Animal Sciences

A group of proteins called heat shock proteins (HSPs) are synthesized during heat stress. They are expressed at high levels when exposed to stress. Polymorphism in HSPs has been reported to be associated with heat tolerance and reproductive performance in cattle. The present study aimed to investigate the polymorphisms in the coding region of the HSPA1A gene in Holstein-Friesian (HF) crossbreds and the Deoni breed of cattle. Genomic DNA was extracted from 94 animal blood samples and was subjected to polymerase chain reaction-single strand conformation polymorphism analysis, which revealed 14 band patterns in Deoni cattle and 8 band patterns in …


Genetic Diversity Of Village Chickens In Central Black Sea Region And Commercial Chickens In Turkey By Using Microsatellite Markers, Levent Mercan, Ahmet Okumuş Jan 2015

Genetic Diversity Of Village Chickens In Central Black Sea Region And Commercial Chickens In Turkey By Using Microsatellite Markers, Levent Mercan, Ahmet Okumuş

Turkish Journal of Veterinary & Animal Sciences

Local chicken populations in the Central Black Sea Region of Turkey have been intensively affected by governmental poultry culling due to avian influenza outbreak risks. The aim of this study was to investigate the genetic diversity of indigenous chicken populations raised in the Central Black Sea Region in order to assess genetic structures of these populations and to determine genetic relationships between the study populations and certain commercial chicken genotypes. Genotypic diversity of 45 Turkish village chicken populations located in 5 provinces in the Central Black Sea Region of Turkey and 2 commercial hybrid populations were compared using 28 autosomal …


Serological And Molecular Diagnosis Of Paratuberculosis In Dairy Cattle, Kadi̇r Semi̇h Gümüşsoy, Tuba İça, Seçi̇l Abay, Fuat Aydin, Harun Hizlisoy Jan 2015

Serological And Molecular Diagnosis Of Paratuberculosis In Dairy Cattle, Kadi̇r Semi̇h Gümüşsoy, Tuba İça, Seçi̇l Abay, Fuat Aydin, Harun Hizlisoy

Turkish Journal of Veterinary & Animal Sciences

In this study, the presence of paratuberculosis was investigated by serological and molecular methods in herds of dairy cattle. Blood, milk, and stool samples of 147 cows aged 2 years old or older with chronic diarrhea were collected. A California mastitis test (CMT) was performed on milk samples. Indirect paratuberculosis enzyme-linked immunosorbent assay (ELISA) test was used for serological investigation. Polymerase chain reaction (PCR) was utilized for molecular identification of Mycobacterium avium subsp. paratuberculosis (MAP) from milk and stool samples. Immunomagnetic separation (IMS) method was used for milk and fecal samples. Eighteen (12.24%), 44 (29.93%), 36 (24.49%), 28 (19.05%), and …


Impact Of B. Melitensis Rev-1 Vaccination On Brucellosis Prevalence, Adosinda Maria Coelho, Maria De Lurdes Pinto, Juan Garcia Diez, Ana Claudia Coelho Jan 2015

Impact Of B. Melitensis Rev-1 Vaccination On Brucellosis Prevalence, Adosinda Maria Coelho, Maria De Lurdes Pinto, Juan Garcia Diez, Ana Claudia Coelho

Turkish Journal of Veterinary & Animal Sciences

Trás-os-Montes e Alto Douro, in the northeastern region of Portugal, previously exhibited the highest brucellosis prevalence in the entire small ruminant population of Portugal. Consequently, a vaccination program of the whole population with Brucella melitensis Rev-1 was carried out from 2001 to 2004, and further compulsory Rev-1 vaccination of 3- to 6-month-old lambs and kids was carried out between 2005 and 2007. The prevalence of brucellosis decreased by 71.42% in 2004, with a 2-fold decrease occurring from 2005 to 2007. The reduction of brucellosis prevalence was statistically significant (P < 0.001) according to flock size. By species, brucellosis prevalence decreased 14.5-fold in sheep and 5.5-fold in goats in 2004. Regarding flock constitution, it decreased by 77% for pure flocks and 73% in mixed flocks in 2004. Regarding the animals involved in production, brucellosis prevalence decreased during the 7-year period. It was concluded that Rev-1 vaccination of the whole population was effective in decreasing brucellosis prevalence. These results contribute to the scarce information available regarding the effect of Rev-1 vaccination on different characteristics of flocks. They may be used to improve the efficiency of brucellosis eradication programs within livestock management.


The Assembly Of Virus-Like Particles Of Porcine Transmissible Gastroenteritisvirus In Vitro By Baculovirus Expression System, Song Zhenhui, Haibo Feng, Zhi Zhu, Xianjin Dai, Yue Zhou, Yuntian Li, Xinzhi Cao Jan 2015

The Assembly Of Virus-Like Particles Of Porcine Transmissible Gastroenteritisvirus In Vitro By Baculovirus Expression System, Song Zhenhui, Haibo Feng, Zhi Zhu, Xianjin Dai, Yue Zhou, Yuntian Li, Xinzhi Cao

Turkish Journal of Veterinary & Animal Sciences

Virus-like particles (VLPs) produced by recombinant expression of the major viral structural proteins could be an attractive method for transmissible gastroenteritis virus (TGEV) assembly and release. In this study, the M gene, sM gene, and N gene of TGEV were inserted into donor plasmid pFastBac Dual. Those recombinant donor plasmids were transformed into E.coli DH10Bac respectively. Recombinant baculoviruses were obtained by transfection of the Sf9 cells with Bacmid-M, Bacmid-sM, and Bacmid-N. The results of indirect immunofluorescence assay showed that recombinant M protein, sM protein, and N protein were expressed successfully. In vitro experiments of VLP assembly of TGEV were performed …


The Effect Of Macromolecule And Growth Factor Combinations On In Vitrodevelopment Of Bovine Embryos, Alper Koçyi̇ği̇t, Mesut Çevi̇k, Uğur Şen, Mehmet Kuran Jan 2015

The Effect Of Macromolecule And Growth Factor Combinations On In Vitrodevelopment Of Bovine Embryos, Alper Koçyi̇ği̇t, Mesut Çevi̇k, Uğur Şen, Mehmet Kuran

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to determine the effects of different macromolecule sources added to synthetic oviduct fluid (SOF) culture medium supplemented with growth factors on the development of bovine embryos and blastocyst morphology. Zygotes were distributed into 5 treatment groups. Cleavage, morula, and blastocyst rates were evaluated under a stereomicroscope. Trophectoderm (TE) and inner cell mass (ICM) cells were determined by differential staining method. It was found that bovine serum albumin (BSA), either alone or in combination with growth factors, as compared to the control or polyvinyl-alcohol (PVA) resulted in higher embryo yield and faster development during early bovine embryo …


Y Chromosome Haplotype Characterization Of Tunisian Sheep Breeds, Samia Kdidi, Mohamed Habib Yahyaoui, Belen García-Manrique, Pilar Sarto, Mohsen Ben Sassi, Touhami Khorchani, Jorge Hugo Calvo Jan 2015

Y Chromosome Haplotype Characterization Of Tunisian Sheep Breeds, Samia Kdidi, Mohamed Habib Yahyaoui, Belen García-Manrique, Pilar Sarto, Mohsen Ben Sassi, Touhami Khorchani, Jorge Hugo Calvo

Turkish Journal of Veterinary & Animal Sciences

This study aimed to investigate Y chromosomal haplotypes in the main Tunisian sheep breeds. For this purpose, we sequenced 2 regions of SRY (549-bp and 598-bp fragments) and 1 region in each of the AMELY and DBY genes in Barbarin, Western Thin Tail, Sicilo Sarde, and Black Thibar breeds. In addition, we analyzed the diversity at the SRYM18 microsatellite locus and detected 4 alleles of 139, 141, 143, and 145 bp. The sequencing analysis did not reveal any polymorphism in the AMELY or DBY regions; however, 2 samples carried the G allele in the 549-bp fragment of SRY. A total …


Relationships Of Fatty Acid Group Contents In Milk And Reproductiveperformance In Holstein Cows, Jaromír Duchácek, Jan Beran, Martin Ptácek, Ludek Stádník, Monika Okrouhla, Renata Tousová, Martina Dolezalova Jan 2015

Relationships Of Fatty Acid Group Contents In Milk And Reproductiveperformance In Holstein Cows, Jaromír Duchácek, Jan Beran, Martin Ptácek, Ludek Stádník, Monika Okrouhla, Renata Tousová, Martina Dolezalova

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to evaluate the relationships between milk fatty acid group contents and subsequent reproductive performance in Holstein cows. A total of 27 cows in the 1st to 4th lactations were included in the evaluation. Cows were divided into primiparous and multiparous groups. Daily milk yields increased in the first 8 weeks of lactation. Body condition score decreased until the third month of lactation. As the cows gradually recovered from the negative energy balance, the contents of saturated fatty acids (SFAs) increased, whereas the contents of monounsaturated fatty acids (MUFAs) and polyunsaturated fatty acids (PUFAs) decreased. …


Textural Acceptability Of Prepared Fish Sausages By Controlling Textural Indicators, Mehmet Tolga Di̇nçer, Şükran Çakli Jan 2015

Textural Acceptability Of Prepared Fish Sausages By Controlling Textural Indicators, Mehmet Tolga Di̇nçer, Şükran Çakli

Turkish Journal of Veterinary & Animal Sciences

The purpose of this paper is to verify the textural quality and acceptability of two different prepared emulsion-type fish sausages that were evaluated by using rainbow trout (Oncorhynchus mykiss) and saithe (Pollachius virens) fillets. Texture properties of the fish sausages were compared with three different emulsion-type sausages (chicken, turkey, and beef sausages) using texture profile analysis (TPA), shear test, Kramer shear test, pate penetration test, cooking loss, and expressible moisture. According to the TPA and firmness values, some textural indicators were found significantly weak (P < 0.05), but they met consumers preferences.


Radionuclide Exposure In Animals And The Public Health Implications, Roseline Yemisi Olobatoke, Manny Mathuthu Jan 2015

Radionuclide Exposure In Animals And The Public Health Implications, Roseline Yemisi Olobatoke, Manny Mathuthu

Turkish Journal of Veterinary & Animal Sciences

Living tissues are usually exposed to varying degrees of radiation from natural and manmade radioactive substances. Depending on the type of radiation emitted and rate and dose of absorption, all radioactive substances are potentially hazardous. Previous researches on the biological effects of radiation as well as preventive measures have focused on human subjects, being the most radiosensitive species. However, exposure of animals to ionizing irradiation may negatively influence their production performance, resulting in some level of economic loss. In addition, many food animals may represent a significant pathway for transfer of radionuclides to humans, thereby adding to the exposure burden. …


Concentrations Of Nefa, Ss-Hba, Triglycerides, And Certain Blood Metabolitesin Healthy Colored Angora Goats During The Peripartum Period, Funda Eşki̇, İbrahi̇m Taşal, Mehmet Aki̇f Karsli, Sai̇t Şendağ, Bariş Atalay Uslu, Henrik Wagner, Axel Wehrend Jan 2015

Concentrations Of Nefa, Ss-Hba, Triglycerides, And Certain Blood Metabolitesin Healthy Colored Angora Goats During The Peripartum Period, Funda Eşki̇, İbrahi̇m Taşal, Mehmet Aki̇f Karsli, Sai̇t Şendağ, Bariş Atalay Uslu, Henrik Wagner, Axel Wehrend

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the changes in serum nonesterified fatty acid (NEFA), serum ß-hydroxybutyric acid (ß-HBA), triglycerides, Ca, Na, and other metabolites (bilirubin, glutamate dehydrogenase (GLDH)) in the blood of grazing, healthy goats at the time of parturition. Blood samples were taken weekly from the jugular vein of 11 goats, starting at week 2 antepartum (ap) until week 9 postpartum (pp). NEFA and ß-HBA concentrations increased from week 2 ap to 2 weeks pp. The increase in NEFA level was not significant; however, the ß-HBA levels were higher (P < 0.05) 2 weeks pp compared to the levels at 2 weeks ap. Triglycerides were recorded at maximum levels (P < 0.05) 2 weeks ap, with the lowest concentrations at 3 weeks pp. Bilirubin levels consistently increased up to 7 weeks pp, followed by a decrease. However, these changes were not significant. Similarly, GLDH activities increased until week 8 pp. A significant difference (P < 0.05) was recorded between the 1st week and 8th week pp. Ca and Na levels were lower during the 1st week pp and increased at 3 weeks pp. The results show that there are characteristic alterations of some metabolic blood parameters in goats around the time of parturition, which may be related to physiological changes.