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Full-Text Articles in Medicine and Health Sciences
Molecular Identification Of Tick-Borne Pathogens In Tickhaemaphysalis Longicornis From Sheep In Henan, China, Yongshuai Peng, Meng Qi, Fuchun Jian, Jinhong Wang, Yali Lv, Jiujian Wei, Rongjun Wang, Longxian Zhang, Changshen Ning
Molecular Identification Of Tick-Borne Pathogens In Tickhaemaphysalis Longicornis From Sheep In Henan, China, Yongshuai Peng, Meng Qi, Fuchun Jian, Jinhong Wang, Yali Lv, Jiujian Wei, Rongjun Wang, Longxian Zhang, Changshen Ning
Turkish Journal of Veterinary & Animal Sciences
Tick-borne diseases are one of the most important classes of disease in animal husbandry and cause severe economic losses. In this study, 660 adult female ticks were collected from sheep in nine localities in Henan Province, China. All were identified as Haemaphysalis longicornis, confirmed with light microscopy and PCR amplification. The pathogens identified in these ticks included bacteria of the genus Anaplasma (Anaplasma ovis, A. bovis, A. phagocytophilum) and piroplasmal protozoans (Theileria luwenshuni, Babesia motasi). Our results show the high prevalence of A. bovis (20.4%, 135/660) and T. luwenshuni (14.4%, 95/660) in the ticks from sheep, whereas A. ovis, A. …
Co-Existence Of Bovine Viral Diarrhea And Border Disease Viruses In A Sheep Flock Suffering From Abortus And Diarrhea, Peli̇n Tuncer Göktuna, Gi̇zem Alpay, Eda Baldan Öner, Kadi̇r Yeşi̇lbağ
Co-Existence Of Bovine Viral Diarrhea And Border Disease Viruses In A Sheep Flock Suffering From Abortus And Diarrhea, Peli̇n Tuncer Göktuna, Gi̇zem Alpay, Eda Baldan Öner, Kadi̇r Yeşi̇lbağ
Turkish Journal of Veterinary & Animal Sciences
Pestivirus infections have a huge economic impact on livestock production. In 2014, an aborted fetus from a sheep flock suffering from abortus and diarrhea was submitted for virological diagnosis. Due to the positive result of the sample for pestivirus and continuing clinical symptoms in the flock, all of the animals were sampled individually. Blood samples for serum and peripheral blood mononuclear cells were collected from 93 animals (5 rams, 26 lambs, and 62 sheep). During sampling 1 ocular and 4 rectal swab samples were obtained from lambs that had a clinical eye problem and diarrhea, respectively. Additionally 5 aborted fetuses …
The Effect Of Combining Vitamin E And C On The Viability Improvement Of Transfected Ovine Spermatogonial Stem Cells After Cryopreservation And Thawing, Hosein Shabani, Mohammad Zandi, Hamideh Ofogi, Mohammad Reza Sanjabi, Khosro Hoseini Pajooh
The Effect Of Combining Vitamin E And C On The Viability Improvement Of Transfected Ovine Spermatogonial Stem Cells After Cryopreservation And Thawing, Hosein Shabani, Mohammad Zandi, Hamideh Ofogi, Mohammad Reza Sanjabi, Khosro Hoseini Pajooh
Turkish Journal of Veterinary & Animal Sciences
The present study aimed to identify whether vitamin E and C and their combination in basal freezing medium improved the viability of postthaw transfected spermatogonial stem cells (SSCs). For this purpose, SSCs were harvested by enzymatic digestion twice. After enrichment and culture on Sertoli cell feeder layer, SSCs were characterized by using alkaline phosphatase staining and expression of oct-4 and c-kit genes as specific stem cell markers. As to the transfection of SSCs, different concentrations of DNA (0.2, 0.4, and 0.8 μg) and turbofect (0.4 and 0.8 μL) in 100 μL of medium were studied. The results showed that cryopreservation …
Comparative Evaluations Of Tear Secretion In Healthy And Infectious Keratoconjunctivitis Romanov Sheep With Schirmer Tear Test And Phenol Red Thread Tear Test, Mustafa Bariş Akgül, Ni̇hat Şindak, Ali̇ Gülaydin, Doğukan Özen, Serpi̇l Kahya Demi̇rbi̇lek, Kadi̇r Sulu
Comparative Evaluations Of Tear Secretion In Healthy And Infectious Keratoconjunctivitis Romanov Sheep With Schirmer Tear Test And Phenol Red Thread Tear Test, Mustafa Bariş Akgül, Ni̇hat Şindak, Ali̇ Gülaydin, Doğukan Özen, Serpi̇l Kahya Demi̇rbi̇lek, Kadi̇r Sulu
Turkish Journal of Veterinary & Animal Sciences
The aim of this study is to determine the amount of tear secretion in sheep with infectious keratoconjunctivitis and healthy ones in the Siirt region by Schirmer tear test and phenol red thread tear test. A total of 47 Romanov sheep, consisting of sheep with infectious keratoconjunctivitis (n = 6) and healthy sheep (n = 41), constituted the study material. Phenol red thread tear test followed by Schirmer tear test was performed on both eyes of the animals, properly taken at a control visit on their feet. The Schirmer tear test and phenol red thread tear test values were measured …