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Corticosteroid Effects And Senescence In Cultured Endothelium, Stephen A. Gudas

Theses and Dissertations

Cultures of human umbilical vein endothelial cells were treated with heparin-corticosteroid combinations to determine effects on cellular growth. Standard proliferation assays, colony formation assays, and cytoflourometric analysis were the methods employed. Dexamethasone (DEX) and hydrocortisone (HC) were inhibitory to growth of HUVEC when EtOH was used as a solvent and fully supplemented medium (20% serum) was employed. When DMSO was the solvent, growth enhancement sometimes occurred when (DEX) was the test steroid; growth inhibition occurred with this steroid when a reduced (1%) serum component was used. Since significant inhibition of cell colony formation in response to DEX administration was observed, …