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Assessing Tumor Physiology By Dynamic Contrast-Enhanced Near-Infrared Spectroscopy, Kyle Verdecchia, Jonathan Elliott, Mamadou Diop, Lisa Hoffman, Ting-Yim Lee, Keith St. Lawrence

Anatomy and Cell Biology Publications

The purpose of this study was to develop a dynamic contrast-enhanced (DCE) near-infrared spectroscopy (NIRS) technique to characterize tumor physiology. Dynamic data were acquired using two contrast agents of different molecular weights, indocyanine green (ICG) and IRDye 800CW carboxylate (IRDcxb). The DCE curves were analyzed using a kinetic model capable of extracting estimates of tumor blood flow (F), capillary transit time (t(c)) and the amount of dye that leaked into the extravascular space (EVS) - characterized by the extraction fraction (E). Data were acquired from five nude rats with tumor xenografts (>10mm) implanted in the neck. Four DCE-NIR datasets …