Open Access. Powered by Scholars. Published by Universities.®

Medicine and Health Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

Analytical, Diagnostic and Therapeutic Techniques and Equipment

University of Texas Rio Grande Valley

Theses/Dissertations

2013

Articles 1 - 1 of 1

Full-Text Articles in Medicine and Health Sciences

Design And Configuration Of Laser Tweezers Microscopy For Force Measurements On Single Dna Molecules, Carlos Jeziel Gonzalez Banuelos Nov 2013

Design And Configuration Of Laser Tweezers Microscopy For Force Measurements On Single Dna Molecules, Carlos Jeziel Gonzalez Banuelos

Theses and Dissertations - UTB/UTPA

In this study, a detailed description of the optical tweezers microscopy technique is presented, as well as the methodologies used to prepare the DNA molecule for mechanical measurements at the nanoscale. The main objective is to initiate and extend the experimental biophysical studies on the DNA-proteins interactions at the University of Texas at Brownsville (UTB). DNA-binding proteins control almost all aspects of cellular function, such as transcription; chromosome maintenance, replication and DNA repair depend on the interaction of proteins with DNA. In view of such an important role played by DNA–protein interactions, various techniques have evolved over the years to …