Medicine and Health Sciences Commons^™

Cloned Microglias With Novel Delivery System In Multiple Sclerosis, Abdul Mannan Baig Dr.

Department of Biological & Biomedical Sciences

Multiple Sclerosis (MS) is a chronic inflammatory neurological disease of the Central Nervous System (CNS), characterized by demyelination and activation of microglia. Mitochondrial mutations and dysfunctions in microglial cells are thought to contribute to the detrimental effects of neuroinflammation seen in MS. The Somatic Nuclear Transfer (SCNT) technology offers a more practical mode of therapy in MS, this method would attempt to dilute and/or progressively replace the mutated and activated microglia with cloned Olfactory Ensheathing Cells (OEC) with remyelinating and scavenging properties which would attempt to limit the progression of MS. Applying SCNTderived Embryonic Stem (ES) cells based therapy by …

Prokinetic And Laxative Effects Of The Crude Methanolic Extract Of Viola Betonicifolia Whole Plant In Rodents, Naveed Muhammad, Najeeb Rehman,, Haroon Khan, Muhammad Saeed, Anwar Gilani

Department of Biological & Biomedical Sciences

Abstract Background: The present study was aimed to provide ethnopharmacological basis for the medicinal use of Viola betonicifolia whole plant in indigestion and constipation. Methods: Mice were used in in-vivo prokinetic and laxative studies while in-vitro experiments were conducted on isolated tissues of rabbit and guinea-pig gut preparations suspended in a tissue bath to measure isotonic contractions. Results: The crude methanolic extract of Viola betonicifolia (VBME) showed partially atropine-sensitive prokinetic (50 and 100 mg/kg) and laxative (30 and 100 mg/kg) activities in mice. When tested in isolated rabbit jejunum and guinea-pig ileum, VBME caused dose-dependent contractions at 0.01-0.3 mg/mL and …

Use Of Inhaled Pge1 To Improve Diastolic Dysfunction, Lvedp, Pulmonary Hypertension And Hypoxia In Ards—A Randomised Clinical Trial, Shahla Siddiqui, Nawal Salahuddin, Salwa Zubair, Muniza Yousuf, Iqbal Azam, Anwar Hassan Gilani

Department of Biological & Biomedical Sciences

Introduction: We wished to see the effects of inhaled PGE1 on diastolic dysfunction, left ventricular end diastolic pressure (LVEDP), pulmonary hypertension and hypoxia in ARDS patients.
Methods: This is a randomized, prospective, clinical trial conducted in the main adult intensive care unit of a tertiary care University hospital. A total of 67 patients were recruited. Inclusion criteria included all adult patients with a P/F ratio /or Pulmonary Artery systolic (Pa) pressures of >35 mmHg on Pulmonary artery catheter or suspected on clinical grounds. A transthoracic echo was performed to record the diastolic function, LVEDP and Pa pressures. Subsequently patients were …

An In Vivo Study On The Diuretic Activity Of Holarrhena Antidysenterica, Aslam Khan, Samra Bashir, Anwar Hassan Gilani

Department of Biological & Biomedical Sciences

Holarrhena antidysenterica is used as diuretic in traditional medicine. The aim of this study was to evaluate the crude extract of H. antidysentrica seeds (Ha.Cr) and its fractions, n-hexane (Ha.Hx), n-butanol (Ha.Bu) and aqueous (Ha.Aq), for their diuretic effect in Wistar rats and to investigate whether the activity is concentrated in any of the fractions. Wistar rats kept on fasting for 24 h with water ad labium, divided into normal, positive control and treated groups were orally given normal saline (20 ml/kg), hydrochlorothiazide (HCT; 10 mg/kg) and different doses of the plant material, respectively. Immediately after dosing, the rats were …

Antispasmodic And Ca++ Antagonist Potential Of Marrubiin, A Labdane Type Diterpene From Phlomis Bracteosa, Javid Hussain, Riaz Ullah, Arif-Ullah Khan, Fazal Mabood, Mohammad Raza Shah, Ahmed Al-Harrasi, Anwar Gilani

Department of Biological & Biomedical Sciences

A tricyclic labdane type diterpene was isolated for the first time from ethyl acetate soluble part of Phlomis bracteosa. Its structure was confirmed by x-ray which was found to be marrubiin. When studied in isolated rabbit jejunum, marrubiin caused concentration-dependent relaxation of spontaneous and high K+ (80 mM)-induced contractions, like that caused by verapamil, indicating that marrubiin exhibits spasmolytic activity, possibly mediated through Ca++ channel blocking action.

In Vitro And In Vivo Anthelmintic Activity Of Terminalia Arjuna Bark, Hafiz Allah Bachaya, Zafar Iqbal, Muhammad Nisar Khan, Abdul Jabbar, Anwar Hassan Gilani, Islam-Ud Din

Department of Biological & Biomedical Sciences

The present study was carried out to evaluate the anthelmintic activity of Terminalia arjuna (Roxb.) bark locally used as an anthelmintic. Lethal median concentration (LC50 values) of methanolic extract of T. arjuna bark in egg hatch and larval development tests against Haemonchus contortus ova and larva were found to be 645.65 and 467.74 μg mL-1, respectively. Inadult motility assay, efficacy of the extract was evident by the mortality of H. contortus at different hours post exposure. Invivo results revealed maximum (87.3%) egg count percent reduction (ECR) in sheep treated with crude methanolic extract @ 3 g kg-1 body …

Hepatitis B Virus Interacts With Albumin Precursor In Vivo, Saeeda Baig, Anwar Ali Siddiqui, Shamim Mushtaq, Jawed Shafqat

Department of Biological & Biomedical Sciences

A range of proteins has been recognized as mediators/hypothetical receptors for hepatitis B virus (HBV), but the results are conflicting and inconclusive especially regarding their biological significance. This study was aimed at identifying a novel HBV-interacting protein which would provide a better understanding of its transport in blood, attachment, fusion and entry into hepatocytes. Serum samples positive for HBV confirmed by PCR were subjected to ammonium sulfate fractionations at 50, 75, 100%, saturation. PCR of each fraction demonstrated amplification of HBV in 100% fraction. Protein analysis by SDS PAGE of fractions showed one band of approximately 69 kDa protein, in …

Determination Of Alpha-I Antitrypsin Genetic Deficiency In Duodenal Ulcer By Polymerase Chain Reaction, A Shahid, H Qureshi, S J. Zuberi, Anwar Ali Siddiqui, T Sultana, M A. Waqar

Department of Biological & Biomedical Sciences

Objective: To confirm alpha-I-AT deficiency status in duodenal ulcer using a combination of PCR and restricted enzyme digestion.

Methods: Fifty patients with endoscopically proven duodenal ulcer and hundred controls with no signs of the disease were included. Alpha-i-AT phenotypes were confirmed by polymerase chain reaction followed by restriction enzyme digestion.

Results:Alpha-I-AT concentration in duodenal ulcer patients showed a mean value of 2.12 ± 0.11g/1 (range: 0.52-3.95 g/1, p

Conclusion: Alpha-1 AT deficiency was found in 10% of duodenal ulcer patients. DNA analysis more accurately resolved the phenotypes as S and Z mutations (JPMA 52:545; 2002).