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Photobiomodulation Of Bovine Oocytes During Maturation Increases Atp Content And Enhances Subsequent Embryonic Development, Kendall Richey
Photobiomodulation Of Bovine Oocytes During Maturation Increases Atp Content And Enhances Subsequent Embryonic Development, Kendall Richey
All Theses
Bovine in-vitro production(IVP) of embryos is a growing field for producing offspring with desirable genetics in the cattle industries. While IVP is effective, oocyte cytoplasmic maturation is compromised, and bovine oocytes matured in-vitro have reduced metabolic activity than those matured in-vivo. Mitochondria are the central unit of oocyte metabolism, producing ATP through OXPHOS. Photobiomodulation is a light treatment reported to improve metabolic activity. In five experiments, we measured the effects of photobiomodulation treatment with red LED at 16-(L-16) and 20 h(L-20) of bovine oocyte in-vitro maturation on subsequent embryonic development, mitochondrial activity and nuclear progression. Bovine COCs were aspirated …