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Molecular, Genetic, and Biochemical Nutrition Commons™
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Articles 1 - 3 of 3
Full-Text Articles in Molecular, Genetic, and Biochemical Nutrition
Bovine Mammary Alveolar Mac-T Cells Afford A Tool For Studies Of Bovine Milk Exosomes In Drug Delivery., Mojisola Ogunnaike
Bovine Mammary Alveolar Mac-T Cells Afford A Tool For Studies Of Bovine Milk Exosomes In Drug Delivery., Mojisola Ogunnaike
Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research
Bovine milk exosomes (BMEs) have attracted attention as vehicles for delivering RNA therapeutics. BMEs originate in mammary alveolar cells. Here, we determined whether bovine mammary alveolar MAC-T cells are a promising tool to assess RNA delivery by BMEs. MAC-T cells exosomes (MAC-T BMEs) and BMEs were harvested by differential ultracentrifugation. Exosome size, morphology, microRNA content and marker proteins were assessed using nanoparticle tracking analysis, transmission electron microscopy, real-time PCR and immunoblot analysis, respectively. MAC-T cells were genetically engineered to secrete MAC-T BMEs endogenously labeled with a near-infrared fluorescent protein and tissue distribution was compared to fluorophore-labeled BMEs following intravenous injection …
Bovine Milk Extracellular Vesicles (Evs) Modification Elicits Skeletal Muscle Growth In Rats, Hailey A. Parry, C. Brooks Mobley, Petey W. Mumford, Matthew A. Romero, Cody T. Haun, Yufeng Zhang, Paul A. Roberson, Janos Zempleni, Arny A. Ferrando, Ivan J. Vechetti, John J. Mccarthy, Michael D. Roberts, Andreas N. Kavazis
Bovine Milk Extracellular Vesicles (Evs) Modification Elicits Skeletal Muscle Growth In Rats, Hailey A. Parry, C. Brooks Mobley, Petey W. Mumford, Matthew A. Romero, Cody T. Haun, Yufeng Zhang, Paul A. Roberson, Janos Zempleni, Arny A. Ferrando, Ivan J. Vechetti, John J. Mccarthy, Michael D. Roberts, Andreas N. Kavazis
Department of Nutrition and Health Sciences: Faculty Publications
The current study investigated how bovine milk extracellular vesicles (EVs) affected rotarod performance and biomarkers of skeletal muscle physiology in young, growing rats. Twenty-eight-day Fisher 344 rats were provided an AIN-93G-based diet for 4 weeks that either remained unadulterated [EVs and RNA-sufficient (ERS; n = 12)] or was sonicated [EVs and RNA-depleted (ERD; n = 12)]. Prior to (PRE) and on the last day of the intervention (POST), animals were tested for maximal rotarod performance. Following the feeding period, the gastrocnemius muscle was analyzed at the histological, biochemical, and molecular levels and was also used to measure mitochondrial function and …
Milk-Derived Exosomes And Metabolic Regulation, Janos Zempleni, Sonal Sukreet, Fang Zhou, Di Wu, Ezra Mutai
Milk-Derived Exosomes And Metabolic Regulation, Janos Zempleni, Sonal Sukreet, Fang Zhou, Di Wu, Ezra Mutai
Department of Nutrition and Health Sciences: Faculty Publications
Exosomes are natural nanoparticles that play an important role in cell-to-cell communication. Communication is achieved through the transfer of cargos, such as microRNAs, from donor to recipient cells and binding of exosomes to cell surface receptors. Exosomes and their cargos are also obtained from dietary sources, such as milk. Exosome and cell glycoproteins are crucial for intestinal uptake. A large fraction of milk exosomes accumulates in the brain, whereas the tissue distribution of microRNA cargos varies among distinct species of microRNA. The fraction of milk exosomes that escapes absorption elicits changes in microbial communities in the gut. Dietary depletion of …