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Agriculture

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TÜBİTAK

2001

Auxin

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Full-Text Articles in Forest Sciences

Isolation And Characterization Of Sequences Homologous To The Tobacco Clone Axi 1 (Auxin Independent) From A Vicia Sativa Nodule Cdna Library, Yeşi̇m Yalçin Mendi̇, Seli̇m Çeti̇ner, Ton Bisseling Jan 2001

Isolation And Characterization Of Sequences Homologous To The Tobacco Clone Axi 1 (Auxin Independent) From A Vicia Sativa Nodule Cdna Library, Yeşi̇m Yalçin Mendi̇, Seli̇m Çeti̇ner, Ton Bisseling

Turkish Journal of Agriculture and Forestry

In this research, partial nucleotide sequences of the axi 1 gene, which is related to auxin perception and transduction, isolated from Vicia sativa using cDNA library screening were investigated. Four V. sativa cDNA clones representing homologous of the tobacco axi 1 (auxin independent) cDNA clone were isolated and characterized. With sequence analysis two different clones were observed, called 10/3a and 16/3b. Sequences about 500 bp from the 5' end and 450 bp from the 3' end were determined from the 10/3a clone. The sequences from the 5' end of cDNA clone 10/3a shared about 58 % similarity at the nucleotide …