Poultry or Avian Science Commons^™

Unique Genome-Wide Transcriptome Profiles Of Chicken Macrophages Exposed To Salmonella-Derived Endotoxin, Ceren Ciraci, Christopher K. Tuggle, Michael J. Wannemeuhler, Dan Nettleton, Susan J. Lamont

Dan Nettleton

Background: Macrophages play essential roles in both innate and adaptive immune responses. Bacteria require endotoxin, a complex lipopolysaccharide, for outer membrane permeability and the host interprets endotoxin as a signal to initiate an innate immune response. The focus of this study is kinetic and global transcriptional analysis of the chicken macrophage response to in vitro stimulation with endotoxin from Salmonella typhimurium-798.

Results: The 38535-probeset Affymetrix GeneChip Chicken Genome array was used to profile transcriptional response to endotoxin 1, 2, 4, and 8 hours post stimulation (hps). Using a maximum FDR (False Discovery Rate) of 0.05 to declare genes as differentially …

Whole Egg Consumption Impairs Insulin Sensitivity In Rat Model Of Obesity And Type 2 Diabetes, Cassondra J. Saande, Megan A. Steffes, Joseph L. Webb, Rudy J. Valentine, Matthew J. Rowling, Kevin Schalinske

Rudy Valentine

Background: The literature regarding the relation between egg consumption and type 2 diabetes (T2D) is inconsistent and there is limited evidence pertaining to the impact of egg consumption on measures of insulin sensitivity. Objective: The objective of this study was to investigate the effect of dietary whole egg on metabolic biomarkers of insulin resistance in T2D rats. Downloaded from https://academic.oup.com/cdn/advance-article-abstract/doi/10.1093/cdn/nzz015/5374517 by Iowa State University user on 28 March 2019 Methods: Male Zucker diabetic fatty rats (n=12; 6 wk of age) and their lean controls (n=12; 6 wk of age) were randomly assigned to a casein- or whole egg-based diet. At …

Transcriptome Analysis In Spleen Reveals Differential Regulation Of Response To Newcastle Disease Virus In Two Chicken Lines, Jibin Zhang, Michael G. Kaiser, Melissa S. Deist, Rodrigo A. Gallardo, David A. Bunn, Terra R. Kelly, Jack C.M. Dekkers, Huaijun Zhou, Susan J. Lamont

Jibin Zhang

Density And Abundance Of Secretive Marsh Birds In Iowa, Tyler Harms, Stephen Dinsmore

Tyler Harms

A decrease in wetland habitats throughout North America has caused a decline in populations of marsh birds. The objective of this study was to estimate population densities and abundances of secretive marsh birds in Iowa. Call-broadcast surveys were conducted in conjunction with distance sampling for eight species of marsh birds at wetlands in three regions of Iowa during 2009 and 2010. Regions were defined by observed microhabitat characteristics which also corresponded to physiographic regions. Region-specific density estimates were obtained using Program Distance for four species of marsh birds for which sufficient detections existed (Pied-billed Grebe [Podilymbus podiceps], Least …

Gene Expression Response To Heat Stress In Two Broiler Lines, Jibin Zhang, Carl J. Schmidt, Susan J. Lamont

Jibin Zhang

The fast growth rate and increased body weight of modern broilers makes them susceptible to heart failure and sensitive to heat stress. To find the genetic causes of these problems, gene expression was measured in hearts of two broiler lines that differed in their response to heat stress. Each line was separated into two groups that were kept under heat stress or at ambient temperature. The comparison of gene expression between the two lines and heat treatments suggests that expression of 325 genes were significantly changed in modern broilers under heat stress, while only 3 genes changed their expression in …

Variation In Avian Pathogenic Escherichia Coli Colonization Levels In Chickens, Melissa Monson, Michael Kaiser, Susan Lamont

Melissa Monson

Colonization levels in five tissues after avian pathogenic Escherichia coli (APEC) inoculation were investigated in chickens to generate phenotypic data for a genome wide association study (GWAS). Bacterial loads were measured in 370 birds and varied among individuals and tissues. Mean bacterial levels were significantly different between tissues (right lung > spleen > left lung and liver > blood). There were also significant correlations in bacterial load between tissues. These data suggest that colonization levels could be used as phenotypes in GWAS and could help identify markers associated with poultry resistance to APEC infections. After verification, these markers could be used for genetic …

Homoplastic Microinversions And The Avian Tree Of Life, Edward Braun, Rebecca Kimball, Kin-Lan Han, Naomi Iuhasz-Velez, Amber Bonilla, Jena Chojnowski, Jordan Smith, Rauri Bowie, Michael Braun, Shannon Hackett, John Harshman, Christopher Huddleston, Ben Marks, Kathleen Miglia, William Moore, Sushma Reddy, Frederick Sheldon, Christopher Witt, Tamaki Yuri

Sushma Reddy

Abstract Background Microinversions are cytologically undetectable inversions of DNA sequences that accumulate slowly in genomes. Like many other rare genomic changes (RGCs), microinversions are thought to be virtually homoplasy-free evolutionary characters, suggesting that they may be very useful for difficult phylogenetic problems such as the avian tree of life. However, few detailed surveys of these genomic rearrangements have been conducted, making it difficult to assess this hypothesis or understand the impact of microinversions upon genome evolution. Results We surveyed non-coding sequence data from a recent avian phylogenetic study and found substantially more microinversions than expected based upon prior information about …

Comment On Faa Rule Revision - Transport Category Aircraft, Paul F. Eschenfelder, Valter Battistoni

Paul F. Eschenfelder

No abstract provided.

Phytogenic Feed Additives As An Alternative To Antibiotic Growth Promoters In Broiler Chickens, G. R. Murugesan, Basharat Syed, Sudipto Haldar, Chasity Pender

G. R. Murugesan

The recent trend toward reduction of antibiotic growth promoters (AGP) in North American poultry diets has put tremendous pressure on the industry to look for viable alternatives. In this context, phytogenic feed additives (PFA) are researched to improve gut health and thereby performance. An experiment was conducted with the objective to evaluate the effects of PFA as an alternative to AGP on small intestinal histomorphology, cecal microbiota composition, nutrient digestibility, and growth performance in broiler chickens. A total of 432, day-old Vencobb 400 broiler chicks were randomly assigned to one of three dietary groups, each consisting of 12 replicate pens …

In Ovo Serial Skeletal Muscle Diffusion Tractography Of The Developing Chick Embryo Using Dti: Feasibility And Correlation With Histology, Zien Zhou, Zachary Delproposto, Lianming Wu, Jianrong Xu, Jia Hua, Yan Zhou, Yongquan Ye, Zishu Zhang, Jiani Hu, E Mark Haacke

Yan Zhou 周彦文档

Abstract Background Magnetic resonance imaging is a noninvasive method of evaluating embryonic development. Diffusion tensor imaging (DTI), based on the directional diffusivity of water molecules, is an established method of evaluating tissue structure. Yet embryonic motion degrades the in vivo acquisition of long-duration DTI. We used a dual-cooling technique to avoid motion artifact and aimed to investigate whether DTI can be used to monitor chick embryonic skeletal muscle development in ovo, and to investigate the correlation between quantitative DTI parameters fractional anisotropy (FA) and fiber length and quantitative histologic parameters fiber area percentage (FiberArea%) and limb length. Results From 84 …

A Critical Review Of Electrical Water-Bath Stun Systems For Poultry Slaughter And Recent Developments In Alternative Technologies, Sara J. Shields, A. B. M. Raj

Sara Shields, PhD

Prior to slaughter, most farmed birds move through a constant-voltage, multiple- bird, electrical water-bath stun system. Using this system subjects live birds to stressful and painful shackling, and the potential exists for them to receive prestun electric shocks and induction of seizures while still conscious. The existing elec- trical water-bath stunner settings, particularly those used in U.S. slaughter plants, are not necessarily based on sound scientific data that they produce a consistent, immediate stun, and research indicates that they are not effective in all birds. Further, in multiple-bird, electrical water-bath systems, birds may miss the stunner completely. Evidence suggests that …

A Comparison Of The Welfare Of Hens In Battery Cages And Alternative Systems, Sara Shields, Ian J.H. Duncan

Sara Shields, PhD

Housing systems for egg-laying hens range from small, pasture-based flocks to large, commercial-scale operations that intensively confine tens of thousands of hens indoors. The overwhelming majority of laying hens used for commercial egg production in the United States are confined in battery cages and provided 432.3 cm2 (67 in2) of space per bird. Cages prevent hens from performing the bulk of their natural behavior, including nesting, perching, dustbathing, scratching, foraging, exercising, running, jumping, flying, stretching, wingflapping, and freely walking. Cages also lead to severe disuse osteoporosis due to lack of exercise. Alternative, cage-free systems allow hens to move freely through …

Differential Expression Of Cell Cycle Regulators During Hyperplastic And Hypertrophic Growth Of Broiler Subcutaneous Adipose Tissue.Pdf, Jibin Zhang, Yeunsu Suh, Young Min Choi, Paula Chen, Michael Davis, Kichoon Lee

Jibin Zhang

Prevalence And Effects Of Mycotoxins On Poultry Health And Performance, And Recent Development In Mycotoxin Counteracting Strategies, G. R. Murugesan, D Ledoux, K Naehrer, F Berthiller, T Applegate, B Grenier, T Phillips, G Schatzmayr

G. R. Murugesan

Extensive research over the last couple of decades has made it obvious that mycotoxins are commonly prevalent in majority of feed ingredients. A worldwide mycotoxin survey in 2013 revealed 81 % of around 3,000 grain and feed samples analyzed had at least one mycotoxin which was higher than the ten-year average (from 2004 to 2013) of 76% in a total of 25,944 samples. The considerable increase in the number of positive samples in 2013 may be due to the improvements in detection methods and their sensitivity. The recently developed liquid chromatography coupled to (tandem) mass spectrometry allows the inclusion of …

Corrosion Casts: A Novel Application Of A Polyurethane Resin (Pu4ii) For Visualizing Eggshell Pore Morphology, Given Harper, Jason P. Murphy, Mark T. Swanson, William B. Jaeckle

Given Harper

The Human/Animal Interface: Emergence And Resurgence Of Zoonotic Infectious Diseases, Michael Greger

Michael Greger, MD, FACLM

Emerging infectious diseases, most of which are considered zoonotic in origin, continue to exact a significant toll on society. The origins of major human infectious diseases are reviewed and the factors underlying disease emergence explored. Anthropogenic changes, largely in land use and agriculture, are implicated in the apparent increased frequency of emergence and reemergence of zoonoses in recent decades. Special emphasis is placed on the pathogen with likely the greatest zoonotic potential, influenzavirus A.

Effects Of Protease, Phytase And A Bacillus Sp. Direct-Fed Microbial On Nutrient And Energy Digestibility, Ileal Brush Border Digestive Enzyme Activity And Cecal Short- Chain Fatty Acid Concentration In Broiler Chickens, G. R. Murugesan, L. F. Romero, M. E. Persia

G. R. Murugesan

Two experiments were conducted to determine the effects of protease and phytase (PP) and a Bacillus sp. direct-fed microbial (DFM) on dietary energy and nutrient utilization in broiler chickens. In the first experiment, Ross 308 broiler chicks were fed diets supplemented with PP and DFM in a 262 factorial arrangement. The 4 diets (control (CON), CON + PP, CON + DFM, and CON + PP + DFM) were fed from 15–21 days of age. In Experiment 1, significant interaction (P#0.01) between PP and DFM on the apparent ileal digestibility coefficient for starch, crude protein, and amino acid indicated that both …

Effects Of Direct-Fed Microbial Supplementation On Broiler Performance, Intestinal Nutrient Transport And Integrity Under Experimental Conditions With Increased Microbial Challenge, G. R. Murugesan, N. K. Gabler, M. E. Persia

G. R. Murugesan

1. The effects of Aspergillus oryzae- and Bacillus subtilis- based direct-fed microbials (DFM) were investigated on the performance, ileal nutrient transport and intestinal integrity of broiler chickens, raised under experimental conditions, with increased intestinal microbial challenge. 2. The first study was a 3 × 2 factorial experiment, with 3 dietary treatments (control (CON), CON + DFM and CON + antibiotic growth promoter) with and without challenge. Chicks were fed experimental diets from 1 to 28 d, while the challenge was provided by vaccinating with 10 times the normal dose of commercial coccidial vaccine on d 9. In a second experiment, …

Influence Of A Direct-Fedmicrobial And Xylanase Enzyme On The Dietary Energy Uptake Efficiency And Performance Of Broiler Chickens, G. R. Murugesan, M. E. Persia

G. R. Murugesan

BACKGROUND: Efficacy of a multi-strain direct-fed microbial product (PoultryStar® ME; PS) and a xylanase enzyme product on the dietary energy utilization efficiency and resulting performance in broiler chickens was evaluated. Apart from performance parameters, cecal and serum metabolites and activities of hepatic enzymes involved in energy metabolism were also determined. Ross 308 chicks were fed one of four experimental diets [control (CON), CON + PS, CON + xylanase and CON + PS + xylanase] using a 2×2 factorial arrangement from 1–21 days of age.

RESULTS: Cecal proportions of propionate and butyrate, as well as total short-chain fatty acid concentration were …

Validation Of The Effects Of Small Differences In Dietary Metabolizable Energy And Feed Restriction In First-Cycle Laying Hens, G. R. Murugesan, M. E. Persia

G. R. Murugesan

An experiment was conducted to evaluate energy utilization of laying hens fed diets containing 2 ME concentrations, using response criteria including performance, BW, abdominal fat pad, and energy digestibility. The experiment was a 2 × 2 factorial with 2 feeding regimens (ad libitum and restriction fed), and 2 dietary ME levels [2,880 kcal/kg of ME (CON); and 2,790 kcal/kg of ME (LME)]. A total of 60 Hy-Line W36 first-cycle laying hens were fed experimental diets, resulting in 15 individually caged hens for each of the 4 treatments. Hens in the restriction-fed group were fed 90 g of feed per day. …

Modeling Parasitism Rate And Parasitism Risk: An Illustration Using A Colonially Nesting Songbird, The Red-Winged Blackbird Agelaius Phoeniceus, Max Post Van Der Burg, Larkin A. Powell, Andrew J. Tyre

Andrew J Tyre

Ornithologists interested in the drivers of nest success and brood parasitism benefit from the development of new analytical approaches. One example is the development of so-called “log exposure” models for analyzing nest success. However, analyses of brood parasitism data have not kept pace with developments in nest success analyses. The standard approach uses logistic regression which does not account for multiple parasitism events, nor does it prevent bias from using observed proportions of parasitized nests. Likewise, logistic regression analyses do not capture fine scale temporal variation in parasitism. At first glance, it might be tempting to apply log exposure models …

A Study Of Mexican Free-Tailed Bat Chirp Syllables: Bayesian Functional Mixed Modeling Of Nonstationary Time Series Data With Time-Dependent Spectra, Josue G. Martinez, Kirsten M. Bohn, Raymond J. Carroll, Jeffrey S. Morris

Jeffrey S. Morris

We describe a new approach to analyze chirp syllables of free-tailed bats from two regions of Texas in which they are predominant: Austin and College Station. Our goal is to characterize any systematic regional differences in the mating chirps and assess whether individual bats have signature chirps. The data are analyzed by modeling spectrograms of the chirps as responses in a Bayesian functional mixed model. Given the variable chirp lengths, we compute the spectrograms on a relative time scale interpretable as the relative chirp position, using a variable window overlap based on chirp length. We use 2D wavelet transforms to …

New Peril + Old Promises = Bad Results, Paul Eschenfelder

Paul F. Eschenfelder

No abstract provided.

Erau Spring 2013 Newsletter, Paul Eschenfelder

Paul F. Eschenfelder

No abstract provided.

Avma Guidelines For The Euthanasia Of Animals: 2013 Edition, Steven Leary, Wendy Underwood, Raymond Anthony, Samuel Cartner, Douglas Corey, Temple Grandin, Cheryl B. Greenacre, Sharon Gwaltney-Bran, Mary Ann Mccrackin, Robert Meyer, David Miller, Jan Shearer, Roy Yanong

Cheryl B Greenacre DVM

No abstract provided.

New Model For Examining The Energy Metabolism Of Laying Hens, G. Murugesan, Michael Persia

G. R. Murugesan

An experiment was conducted to evaluate the energy utilization of laying hens fed diets containing two energy concentrations, using a holistic approach includingmeasurement of productive, maintenance and storage energy. The experiment was a 2 x 2 factorial arrangementwith two feeding levels (ad libitum and restricted feeding of 90 g feed/hen-d), and two dietary energy levels (HE-high metabolizable energy or ME content of 2,880 kcal/kg; and LE-low ME content of 2,790 kcal/kg). A total of 60 HyLine W36 first-cycle laying hens were fed treatment dietsfor 12 wk from hen age 27 to 39 wk, with 15 individually housed hens for each …

Evaluation Of Energy Values Of Various Oil Sources When Fed To Broiler Chicks, G. Murugesan, Brian Kerr, Michael Persia

G. R. Murugesan

The nitrogen-corrected apparent metabolizable energy (AMEn) values of seven different oil and fat sources used in broiler diets, primarily across the Midwestern US, were determined in a digestibility experiment. Fifteen days old, Ross 308 male broiler chicks were fed diets containing each oil or fat source at 0%, 3%, 6%, and 9% inclusion levels for 7 days before excreta samples were collected to analyze AMEn on day 21. The AMEn was calculated using 2 different methods, including a linear equation slope method as well as calculating the difference between basal diet and oil containing diets. The AMEn values determined by …

Influence Of Latitude On The Winter Abundance Of Red-Tailed Hawks (Buteo Jamaicensis) And American Kestrels (Falco Sparverius) In Illinois, Given Harper, Anna Groves, Vic Berardi, Paul Sweet, Jance Sweet, Angelo P. Capparella

Given Harper

Rocky Mountain Birds: Birds And Birding In The Central And Northern Rockies, Paul A. Johnsgard

Paul Johnsgard

“The Rocky Mountain region has fascinated me ever since I traveled to Glacier and Yellowstone National Parks as a teenager, and saw for the first time such wonderful birds as ospreys, American dippers, and Lew­is’s woodpeckers.” This book is in part based on the author’s earlier Birds of the Rocky Moun­tains (1986, revised 2009), but over a third of the original text has been eliminated. The rest has been updated, expanded and modified to be less technical and more useful to birders in the field. Bird enthusiasts will find viewing loca­tions and updated contact information for hundreds of sites in …

The Art And Artistic Legacy Of Louis Agassiz Fuertes, Paul A. Johnsgard

Paul Johnsgard

Perhaps some Nebraska birders will not immediately recognize the name Louis Agassiz Fuertes, as he died almost 80 years ago. Yet he influenced the art of bird painting as much as did John James Audubon, and provided wonderful artwork for many major state bird reference books. He also personally tutored George Miksch Sutton, the Nebraska-born artist and biologist who provided the NOU with its Burrowing Owl logo, and who contributed greatly to American ornithology, especially that of the southern Great Plains. It is interesting that, like John James Audubon, Roger Tory Peterson, and George Miksch Sutton, we would never think …