Dairy Science Commons^™

J. C. Penney: The Man, The Store And American Agriculture, David Delbert Kruger

David Delbert Kruger

Revised Alfalfa Fertilizer Recommendations For A Changing Environment Greatly Improves Profitability, Brian Lang, John E. Sawyer, Daniel Barker, Ken Pecinovsky

John E. Sawyer

During the last decade, an ever-increasing percentage of alfalfa fields in northeast Iowa have been exhibiting signs of reduced growth and vigor, resulting in declining profitability. Iowa State University Extension in northeast Iowa initiated an investigation into this problem. Sixteen research trials conducted in northeast Iowa over the next 6 years as both on-farm trials and at the northeast ISU research farm uncovered widespread sulfur deficiency problems in alfalfa production. Over 90 ISU Extension educational meetings in northeast Iowa, and extensive use of the media, communicated this issue and tools to address it to farmers and agricultural providers. Survey results …

Practical Considerations And Implementation Of Anaerobic Digester Systems, Jenny Pronto, Curt Gooch, Nellie J. Brown

Nellie J. Brown, MS., C.I.H.

This course prepares learners to implement and run an AD system from the planning and design phase to operation and maintenance. It focuses on the design of AD systems; how to successfully interact with a designer so that the farm’s needs are met; and basic operation and maintenance.

Dealing With Sulfur Deficiency In Northeast Iowa Alfalfa Production, Brian J. Lang, John E. Sawyer, Stephen K. Barnhart

John E. Sawyer

Sulfur deficiencies have been shown to be associated with reduced alfalfa production. This paper documents this problem in NE IA, presenting research over the past 2 years, and provides recommendations for plant analysis as well as appropriate sulfur supplementation strategies.

Effects Of Feeding Rumen-Degradable Valine On Milk Production In Late-Lactating Dairy Cows, Kayla Hultquist, David Casper

David Casper

The study objective was to determine if feeding the rumen-degradable AA Val can increase milk production comparable to recombinant bovine somatotropin (bST). Eight multiparous late-lactating (255 ± 26.4 d in milk) Holstein dairy cows were blocked by milk yield (34.1 ± 8.25 kg/d) and randomly assigned to 1 of 4 treatments in a replicated 4 × 4 Latin square design with 21-d periods (7 d for dietary adaptation and 14 d for data collection). Treatments were control (CON), a single injection of recombinant bST (rbST), and Val fed at 40 (V40) and 80 g/d (V80). Cows were fed a total …

Development Of Mathematical Models To Predict Volume And Nutrient Composition Of Fresh Manure From Lactating Holstein Cows, J.A.D. Ranga Niroshan Appuhamy, L.E. Moraes, L.E. Moraes, C. Wagner-Riddle, David Casper, J. France, E. Kebreab

David Casper

Organic compounds in dairy manure undergo a series of reactions producing pollutants such as ammonia and methane. Because various organic compounds have different reaction rates, the emissions could be accurately determined if amounts and concentrations of individual nutrients in manure are known. A set of empirical models were developed for predicting faecal and urinary water, carbon (C), nitrogen (N), acid detergent fibre and neutral detergent fibre output (kg/day) from lactating Holstein cows. Dietary nutrient contents, milk yield and composition, bodyweight, age and days in milk were used with or without dry matter intake (DMI) as potential predictor variables. Multi-collinearity, goodness …

Prevalence And Knowledge Of Brucellosis In Dairy Cattle In Makuyu Division, Murang’A County, Kenya, Dr. Michael N. Gicheru, Dr. Eric Mwangi, Mwangi Rachel Mbaire

Innovative Research Publications IRP India

Brucellosis is a cosmopolitan zoonotic disease that affects man, domestic animals and wildlife. The bacteria Brucella abortus is the principle cause of brucellosis in cattle. The incidence of the disease in humans, and which directly relates to that in other animals, is highly dependent on animal husbandry practices, the interaction between humans and animals, living standards, hygiene, food customs, and animal and human population density. Makuyu Division where the study was undertaken experiences hot and dry climate, the area has no permanent rivers and domestic water is obtained from boreholes. Most families live in congested homes and are provided with …

Tb181: 2000 Milk Processing Costs In Maine, Timothy J. Dalton, George K. Criner, John Halloran

George K. Criner

The objective of this study is to provide an estimate of the theoretically lowest achievable costs of processing and distributing milk in Maine. This processing and distribution margin (referred hence as "margin") is estimated for a state-of-the-art processing plant assumed to be located in the Portland, Maine, area. The plant is assumed to produce and distribute a line of products including white milk, chocolate milk, orange juice, and other fruit drinks, and to distribute additional purchased products such as cheeses and yogurts. This report will present information for four plants to better study the impact of plant size and production …

B853: Cost Of Producing Milk In Maine: Results From The 2010 Dairy Cost Of Production Survey, Richard Kersbergen, Gary Anderson, George Criner, Anthony Davis

George K. Criner

The present study reports on a survey conducted for the Maine Milk Commission to determine the cost of milk production for 2010. This assessment was undertaken sooner than the normal three-year update because of the difficult economic climate for dairy farms in Maine.

Tb204: Organic Milk Production In Maine: Attributes, Costs, And Returns, Amelia L. Cook, Patrick S. Heacock, George K. Criner, Lisa A. Bragg

George K. Criner

This report summarizes attributes, costs, and returns for organic dairy farms in Maine that responded to the 2008 dairy cost of production survey. This survey and analysis was conducted by the University of Maine in cooperation with the Maine Milk Commission. This publication reports on data collected over the 2007 production year. Analysis and discussion of the data revolve around four categories. The first category averages all 30 organic farms to create a statewide group. The authors then broke these 30 farms into three size groups (small, medium, and large) based on the number of cows on each farm. There …

Prediction Of Total Phenols, Condensed Tannins, And 3-Deoxyanthocyanidins In Sorghum Grain Using Near-Infrared (Nir) Spectroscopy, L. Dykes, L. Hoffmann, O. Portillo-Rodriguez, W.L., Rooney, L.W. Rooney

Elvis A. Heinrichs

The reported high phenolic levels in sorghum have led an interest from sorghum breeding programs in developing and identifying germplasms with high phenolic levels, which require screening a large number of samples to find those with the highest levels. Since wet chemistry screening methods are slow, expensive, and destructive, the use of NIR calibration curves could be an alternative. The objectives of this project were to determine the variation range in total phenols, condensed tannins, and 3-deoxyanthocyanidin levels in a diverse set of sorghum genotypes and to assess the predictive value of NIR curves to estimate these compounds in sorghum. …

Physical Properties Of Yogurt Fortified With Various Commercial Whey Protein Concentrates, Isabelle Sodini, John Montella, Phillip S. Tong

Phillip S. Tong

The effects of whey protein concentrates on physical and rheological properties of yogurt were studied. Five commercial whey protein concentrates (340 g kg−1 protein nominal) were used to fortify milk to 45 g protein kg−1. Fermentation was performed with two different starters (ropy and non-ropy). Resulting yogurts were compared with a control yogurt enriched with skim milk powder. The water-holding capacity of the yogurt fortified with skim milk powder was 500 g kg−1 and ranged from 600 to 638 g kg−1 when fortified with whey protein concentrates. Significant rheological differences have been noticed between the yogurts fortified with different whey …

Method And System For Lactose-Free Or Lactose-Reduced Milk And Associated Products, Production Thereof, And Associated Processes, Joseph H. Hotchkiss, Joey Talbert

Joey Talbert

A system and method capable of hydrolyzing lactose, where the system includes a support formed from a functionalized hydrophobic polymer that is covalently linked to a hydrophilic molecule covalently that is, in turn, covalently linked to an enzyme such as lactose. The method includes the steps of functionalizing a hydrophobic polymer support, covalently linking a hydrophilic molecule to said functionalized polymer support, and covalently linking an enzyme such as lactase to said hydrophilic molecule. The system and method generally relate to the field of food science and engineering and, more particularly to dairy-based food products and their production including solutions …

Viability Of Lactic Acid Bacteria And Sensory Evaluation In Cinnamomum Verum And Allium Sativum-Bio-Yogurts Made From Camel And Cow Milk, Ahmad Salihin Hj Baba, Shori A. B.

Ahmad Salihin Hj Baba

The present study investigate the effect of herbal water extract prepared from Allium sativum and Cinnamomum verum on the viability of lactic acid bacteria (Lactobacillus spp and Streptococcus thermophilus) in cow- and camel-milk yogurts during 21 day refrigerated storage. The organoleptic properties of fresh-yogurts were evaluated. Lactobacillus spp count for fresh cow milk-yogurts (0 day) in both present and absent of C. verum and A. sativum was ranged from 1.4×0 6 to 2.1×10 6 cfu/mL. These values were not significantly changed throughout the 21 days of refrigerated storage. Lactobacillusspp count in fresh plain camel milk- yogurt was 13.2×10 6 cfu/mL …

Extraction Of Lipids From Buttermilk Using Supercritical Carbon Dioxide, Harit K. Vyas, Johanna C. Astaire, Rafael Jiménez-Flores

Rafael Jiménez-Flores

Abstract of paper presented at the 2002 Joint Annual Meeting of the American Dairy Science Association & the American Society of Animal Science.

Disulfide Bonding Patterns Between Β-Lactoglobulin And Κ-Casein In A Heated And Spray-Dried Milk-Model, Annie Bienvenue, C. S. Norris, Michael J. Boland, Lawrence K. Creamer, Rafael Jiménez-Flores

Rafael Jiménez-Flores

Abstract of paper presented at the 2002 Joint Annual Meeting of the American Dairy Science Association & the American Society of Animal Science.

Mycotoxins In Corn Distillers Grains: A Concern In Ruminants?, Alvaro Garcia, Kenneth Kalscheur, Arnold Hippen, David Schingoethe, Kurt A. Rosentrater

Kurt A. Rosentrater

Themain fungi that produce toxins during storage belong to three genera: Aspergillus, Fusarium, and Penicillium. When dealing with cattle diets, it is not easy to correlate the presence of mycotoxins to that of molds. The same types of molds can produce different types of toxins, and different types ofmolds can produce the samemycotoxin.

Caspase 3-Dependent Cell Death Of Neurons Contributes To The Pathogenesis Of West Nile Virus Encephalitis, M. A. Samuel, John D. Morrey, M. S. Diamond

John D. Morrey

West Nile virus (WNV) is a neurotropic, arthropod-borne flavivirus that has become a significant global cause of viral encephalitis. To examine the mechanisms of WNV-induced neuronal death and the importance of apoptosis in pathogenesis, we evaluated the role of a key apoptotic regulator, caspase 3. WNV infection induced caspase 3 activation and apoptosis in the brains of wild-type mice. Notably, congenic caspase 3–/– mice were more resistant to lethal WNV infection, although there were no significant differences in the tissue viral burdens or the kinetics of viral spread. Instead, decreased neuronal death was observed in the cerebral cortices, brain stems, …

Defining Limits Of Treatment With Humanized Neutralizing Monoclonal Antibody For West Nile Virus Neurological Infection In A Hamster Model, John D. Morrey, V. Siddharthan, Aaron L. Olsen, H. Wang, Justin G. Julander, Jeffery O. Hall, H. Li, J. L. Nordstrom, S. Koenig, S. Johnson, M. S. Diamond

John D. Morrey

A potent anti-West Nile virus (anti-WNV)-neutralizing humanized monoclonal antibody, hE16, was previously shown to improve the survival of WNV-infected hamsters when it was administered intraperitoneally (i.p.), even after the virus had infected neurons in the brain. In this study, we evaluated the therapeutic limit of hE16 for the treatment of WNV infection in hamsters by comparing single-dose peripheral (i.p.) therapy with direct administration into the pons through a convection-enhanced delivery (CED) system. At day 5 after infection, treatments with hE16 by the peripheral and the CED routes were equally effective at reducing morbidity and mortality. In contrast, at day 6 …

Efficacy Of Orally Administered T-705 On Lethal Avian Influenza A (H5n1) Virus Infections In Mice, R. W. Sidwell, Dale L. Barnard, C. W. Day, Donald F. Smee, K. W. Bailey, M. H. Wong, John D. Morrey, Y. Furuta

John D. Morrey

T-705 (6-fluoro-3-hydroxy-2-pyrazinecarboxamide) was inhibitory to four strains of avian H5N1 influenza virus in MDCK cells, with the 90% effective concentrations ranging from 1.3 to 7.7 µM, as determined by a virus yield reduction assay. The efficacy was less than that exerted by oseltamivir carboxylate or zanamivir but was greater than that exerted by ribavirin. Experiments with mice lethally infected with influenza A/Duck/MN/1525/81 (H5N1) virus showed that T-705 administered per os once, twice, or four times daily for 5 days beginning 1 h after virus exposure was highly inhibitory to the infection. Dosages from 30 to 300 mg/kg of body weight/day …

Neutralizing Viruses In Suspensions By Copper Oxide-Based Filters, G. Borkow, R. W. Sidwell, Donald F. Smee, Dale L. Barnard, John D. Morrey, H. H. Lara-Villegas, Y. Shemer-Avni, J. Gabbay

John D. Morrey

We report the capacity of copper oxide-containing filters to reduce infectious titers of a panel of viruses spiked into culture media. Enveloped, nonenveloped, RNA, and DNA viruses were affected, suggesting the possibility of using copper oxide-containing devices to deactivate a wide spectrum of infectious viruses found in filterable suspensions.

Enhanced Antiscrapie Effect Using Combination Drug Treatment, D. A. Kocisko, B. Caughey, John D. Morrey, R. E. Race

John D. Morrey

Combination treatment with pentosan polysulfate and Fe(III)meso-tetra(4-sulfonatophenyl)porphine in mice beginning 14 or 28 days after scrapie inoculation significantly increased survival times. This increase may be synergistic, implying that the compounds act cooperatively in vivo. Combination therapy may therefore be more effective for treatment of transmissible spongiform encephalopathies and other protein-misfolding diseases.

A Porphyrin Increases Survival Time Of Mice After Intracerebral Prion Infection, D. A. Kocisko, W. S. Caughey, R. E. Race, G. Roper, B. Caughey, John D. Morrey

John D. Morrey

Prion diseases, including scrapie, are incurable neurodegenerative disorders. Some compounds can delay disease after a peripheral scrapie inoculation, but few are effective against advanced disease. Here, we tested multiple related porphyrins, but only Fe(III)meso-tetra(4-sulfonatophenyl)porphine injected into mouse brains after intracerebral scrapie inoculation substantially increased survival times.

Phosphorothioate Di- And Trinucleotides As A Novel Class Of Anti-Hepatitis B Virus Agents, R. P. Iyer, Y. Jin, A. Roland, John D. Morrey, S. Mounir, B. Korba

John D. Morrey

Several nucleoside analogs are under clinical development for use against hepatitis B virus (HBV). Lamivudine (3TC), a nucleoside analog, and adefovir dipivoxil (ADV), an acyclonucleotide analog, are clinically approved. However, long-term treatment can induce viral resistance, and following the cessation of therapy, viral rebound is frequently observed. There continues to be a need for new antiviral agents with novel mechanisms of action. A library of more than 600 di- and trinucleotide compounds synthesized by parallel synthesis using a combinatorial strategy was screened for potential inhibitors of HBV replication using the chronically HBV-producing cell line 2.2.15. Through an iterative process of …

Anti-Hepatitis B Virus Activity Of Ori-9020, A Novel Phosphorothioate Dinucleotide, In A Transgenic Mouse Model, R. P. Iyer, A. Roland, Y. Jin, S. Mounir, B. Korba, Justin G. Julander, John D. Morrey

John D. Morrey

ORI-9020, a novel dinucleotide, evaluated in transgenic mice expressing hepatitis B virus (HBV), significantly reduced liver HBV DNA (P <= 0.001). Levels of HBeAg and HBsAg in serum and of HBcAg in liver were not affected by treatment. A minimal effective dosage was determined to be between 1.6 and 0.5 mg/kg of body weight/day, which was similar to that observed for adefovir dipivoxil.

Growth And Reproductive Performance Of Small Ruminants Under Integrated Livestock Oil Palm Production System, Ahmad Salihin Hj Baba, Azillah A., Mukherjee T. K., Abdullah R. B.

Ahmad Salihin Hj Baba

The effects of supplementation with mixed fodder or concentrate were investigated on the performance of a) growth of male lambs;md kids grazing extensively under oil palm trees and b) reproduction of ewes and goats stall-fed with native herbage. Supplemented kids and lambs had higher average daily weight gain than controls but the effect was only significant for those which received concentrate (p < 0.05). Final body weight was only significantly different from controls for lambs supplemented with concentrate (p < 0.05). The ages at puberty, first mating, first conception and first kidding of supplemented goats were about 110 days earlier than those for controls (p < 0.05 or better). Supplemented goats had first mating and conceived at lower body weights (p < 0.01) than those in control groups. Different feeding regimes had no effects (p > 0.05) on the reproductive performance of ewes apart fi om highest body weight of first lambing in animals supplemented with concentrate (p < 0.05). Native herbage available under oil palm trees of 5 years old was sufficient for growth …