Life Sciences Commons^™

Comparative Effects Of Different Types Of Biochar On Physical Properties Of Soil And Growth Of Maize, Muhammad Iqbal, Muhammad Yasir, Atif Javed, Sarmad Tausif Ahmad

Journal of Bioresource Management

The current pot trial was conducted to estimate the impacts of different types of biochar on the growth and nutrients availability of maize (Zea mays) and their effects on the properties of soil. Treatments including four different feedstock based biochar i.e wheat straw, rice husk, corn cob and wood bark were applied to the soil in 10 kg pots @ 1.5% w/w. The experiment was carried out using complete randomized design (CRD). The crop was harvested after the plants have completed their vegetative growth. Physiological parameters of the crop (plant height, leaf area, chlorophyll content) were measured before …

Identification Of Blda As A Major Regulator Of Virulence In The Sweetpotato Soil Rot Pathogen Streptomyces Ipomoeae And Characterization Of Distinct Clostridium Sweetpotato Soft Rot Isolates, Kuei-Ting Yang

LSU Doctoral Dissertations

Streptomyces ipomoeae is the causative agent of Streptomyces soil rot on sweetpotato, a disease characterized by extensive necrosis of both adventitious and storage roots. While Streptomyces potato scab pathogens produce a phytotoxin (thaxtomin A), which is induced in the presence of cellobiose and suberin, S. ipomoeae produces a less-modified, phytotoxin (thaxtomin C), whose inducer has not been identified. To investigate transcriptional regulation of thaxtomin C production, we inserted a promoter involved in thaxtomin C synthesis upstream of the gusA reporter in an S. ipomoeae strain. Reporter gene expression was significantly upregulated in the presence of size-fractionated sweetpotato extract (SPE). The …

Evaluating The Current Weed Community In Wild Blueberry Fields And Ipm Strategies For Spreading Dogbane (Apocynum Androsaemifolium), Anthony G. Ayers

Electronic Theses and Dissertations

Lowbush blueberry (Vaccinium angustifolium) is Maine’s third largest crop (USDA 2020 a). From 2017 – 2019 the three seasons yield average was 27,200 tons were harvested from 19,500 acres for a value of $22,468,000 (USDA 2020 c). Lowbush blueberries are managed on a two-year cycle. Every other year, lowbush blueberry fields are pruned to the ground either through the use of a tractor mounted flail mower or a prescribed burn (Yarborough 2009). Pruning is a necessary part of managing lowbush blueberries as the second-year growth produces the highest yield but steadily declines in subsequent years (Drummond et al. …

Inaction On Lead Despite The Relevant Knowledge: Predictors, Covariates, And Outreach Implications, Alessandra Rossi, Bernabas Wolde, Pankaj Lal, Melissa Harclerode

Department of Biology Faculty Scholarship and Creative Works

Testing residential soil and paint for lead provides actionable information. By showing where and how much lead exists on the residence, it allows one to quantify risk and determine the best ways to reduce exposure along with the corresponding health and financial costs. For these reasons, several federal and state programs offer outreach to audiences on the benefits of testing residential soil and paint for lead. Not all individuals who know about lead’s adverse health effects, however, test their residence for lead, potentially limiting the actionable information that could have helped to reduce their exposure. Such individuals represent a challenge …

Exposing Lettuce Plants To Cyanobacteria In A Closed Hydroponics System To Reduce Cyanobacterial Growth And Production, Emily Eberly

Honors Projects

Sandusky Bay is largely populated by cyanobacterial algal blooms, mainly formed by Planktothrix. Fertilizers containing nitrogen and phosphorus run from agricultural lands into the bay, building up excess nutrients forming eutrophic waters. The Planktothrix feed off these nutrients and grow into algal blooms. To determine a potential solution to the growth of these blooms, I implemented a hydroponics system involving Lactuca Sativa for analysis of Planktothrix growth and productivity. Four different nutrient conditions were added to a Planktothrix-only solution and a solution growing Planktothrix with the lettuce in the hydroponics system. The four conditions consisted of no nutrient …

Genome-Wide Association Mapping And Genomic Prediction Of Anther Extrusion In Cimmyt Hybrid Wheat Breeding Program Via Modeling Pedigree, Genomic Relationship, And Interaction With The Environment, Anil Adhikari, Bhoja Raj Basnet, Jose Crossa, Susanne Dreisigacker, Fatima Camarillo, Pradeep Kumar Bhati, Diego Jarquin, Yann Manes, Amir M.H. Ibrahim

Department of Agronomy and Horticulture: Faculty Publications

Anther extrusion (AE) is the most important male floral trait for hybrid wheat seed production. AE is a complex quantitative trait that is difficult to phenotype reliably in field experiments not only due to high genotype-by-environment effects but also due to the short expression window in the field condition. In this study, we conducted a genome-wide association scan (GWAS) and explored the possibility of applying genomic prediction (GP) for AE in the CIMMYT hybrid wheat breeding program. An elite set of male lines (n = 603) were phenotype for anther count (AC) and anther visual score (VS) across three field …

Development Of Mping-Based Activation Tags For Crop Insertional Mutagenesis, Alexander Johnson, Edward Mcassey, Stephanie Diaz, Jacob Reagin, Priscilla S. Redd, Daymond R. Parrilla, Hanh T. Nguyen, Adrian Stec, Lauren A.L. Mcdaniel, Thomas E. Clemente, Robert M. Stupar, Wayne A. Parrott, C Nathan Hancock

Department of Agronomy and Horticulture: Faculty Publications

Modern plant breeding increasingly relies on genomic information to guide crop improvement. Although some genes are characterized, additional tools are needed to effectively identify and characterize genes associated with crop traits. To address this need, the mPing element from rice was modified to serve as an activation tag to induce expression of nearby genes. Embedding promoter sequences in mPing resulted in a decrease in overall transposition rate; however, this effect was negated by using a hyperactive version of mPing called mmPing20. Transgenic soybean events carrying mPing-based activation tags and the appropriate transposase expression cassettes showed evidence of transposition. Expression analysis …

Soil Health Assessment For The Agroecosystems Of West Tennessee, Surendra Singh

Doctoral Dissertations

Soil health assessment is important for making informed sustainable management decisions in production systems. An established standardized method to quantify soil health is lacking and the validity of the existing methods across agroecoregions and cropping systems is not yet proven. Therefore, this study aims to evaluate the feasibility of widely discussed three soil health tests - Haney’s Soil Health Test (HSHT), Comprehensive Assessment of Soil Health (CASH), and Alabama Soil Health Index (ASHI) to assess soil health in diverse cropping systems of Tennessee. Since these approaches were originally developed for specific agroecoregions, we hypothesized that these tests are not sensitive …

Soil Moisture Sensitivity Of Microbial Processing Of Soil Organic Carbon, Shikha Singh

Doctoral Dissertations

Soil organic carbon (SOC) is the largest terrestrial C pool and understanding SOC decomposition in response to environmental factors is critical for accurate predictions of climatic change. Soil moisture is one of the most important, yet less explored, environmental factors controlling soil microbial respiration. The relationship between soil moisture and respiration also varies with soil texture. Currently, it is difficult to predict feedbacks to climatic changes from changes in soil moisture, as most earth system models lack site-specific, experimentally-derived parameters to represent soil moisture-texture-respiration relationships. The overarching goals of this dissertation are to gain a fundamental understanding of the interactive …

Parallel Strategies To Control Bacterial Panicle Blight Of Rice, Laura Ortega

Graduate Theses and Dissertations

Bacterial Panicle Blight (BPB) of rice is a seed-borne disease caused by the bacterium Burkholderia glumae. This disease has affected rice production worldwide and its effects are likely to become more devastating with the continuous increase in global temperatures especially during the growing season. Field data has shown that the disease has been more prevalent in years when the temperatures have been unusually high especially at night possibly due to the bacterial adaptation to grow at temperatures higher than 40oC. With the continuous rise in global temperatures, it is likely that this disease will be more problematic. The bacterium can …

Genomic Selection Of Forage Agronomic Traits In Winter Wheat, Frank Maulana, Ki-Seung Kim, Joshua D. Anderson, Mark E. Sorrells, Twain J. Butler, Shuyu Liu, P. Stephen Baenziger, Patrick Byrne, Xue-Feng Ma

Department of Agronomy and Horticulture: Faculty Publications

Genomic selection (GS) can improve genetic gain of complex traits in plant breeding. Phenotyping agronomic traits of winter wheat (Triticum aestivum L.) for dualpurpose use is expensive and time-consuming. In this study, we compared the prediction accuracies of four GS models (RR-BLUP, GBLUP, GAUSS, and BL) for forage yield (FY), plant height (PH) and heading date (HD) of the hard winter wheat diversity panel (n = 298) using random and stratified sampling methods. In addition, we determined the appropriate training population (TP) size and marker density for GS of the traits. Moderate to high prediction accuracies ranging from 0.66 …

Fungicide Resistance: Surveillance, Risk Assessment And Evolution In Two Soil-Borne Pathogens, Nikita Gambhir

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Fungicide-resistant pathogens are an increasing threat to fungicide efficacy and plant health. The goal of this dissertation was to advance the foundational knowledge required to prevent and detect fungicide resistance development in the seedling disease pathogen, Rhizoctonia zeae and the white-mold pathogen, Sclerotinia sclerotiorum. Corn and soybean fields in 12 states (IA, IL, IN, KS, KY, MI, MN, MO, ND, NE, SD, and WI) were surveyed for R. zeae. In vitro fungicide sensitivity was determined for 91 isolates to fludioxonil, sedaxane, and/or prothioconazole. Rhizoctonia zeae was sensitive to all fungicides (EC₅₀ < 3 µg/ml) except azoxystrobin (EC₅₀ > 100 µg/ml). In planta application of …

Development And Evaluation Of An Automated Linear Move Fertigation System For Cotton Using Active Remote Sensing, Stewart Bell

All Theses

Optimum nitrogen (N) application is essential to the economic and environmental sustainability of cotton production. Variable-rate N fertigation could potentially help farmers optimize N applications, but current overhead irrigation systems normally lack automated site-specific variable-rate fertigation capabilities. The objective of this research was to develop and evaluate an automated variable-rate N fertigation system based on real-time Normalized Difference Vegetation Index (NDVI) measurements from crop sensors integrated with a lateral move irrigation system. For this purpose, NDVI crop sensors and a flow meter were integrated with Arduino microcontrollers and an automated fertigation system was constructed. A computer program was developed to …

Assessment Of Grain Safety In Developing Nations, Jose R. Mendoza

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Grains are the most widely consumed foods worldwide, with maize (Zea mays) being frequently consumed in developing countries where it feeds approximately 900 million people under the poverty line of 2 USD per day. While grain handling practices are acceptable in most developed nations, many developing nations still face challenges such as inadequate field management, drying, and storage. Faulty grain handling along with unavoidably humid climates result in recurrent fungal growth and spoilage, which compromises both the end-quality and safety of the harvest. This becomes particularly problematic where there is little awareness about health risks associated with poor …

Characterization Of Genetic Sources Associated With Restorability And Seed Dimension In Arkansas Restorer Rice Lines, Ozgur Azapoglu

Graduate Theses and Dissertations

Hybrid rice (Oryza sativa L.) breeding offers a significant opportunity to enhance rice production, and the cultivation of a male sterile line is the most important factor in the success of cross-breeding. One of the key elements of hybrid rice production is to develop a restorer line that is assigned as the male parent. The restorer lines provide viable pollen for fertilization of the male sterile plant due to the presence of a restorer gene (Rf) in their genomes. Any superior restorer line applied to hybrid rice production must contain genes/quantitative trait loci (QTL) associated with the desirable agronomic traits …

Application Of X-Ray Imaging And Glass Transition Principles In Understanding Rice Kernel Fissure Formation, Zephania R. Odek

Graduate Theses and Dissertations

Rice kernels with internal fractures of the endosperm, fissures, tend to break during milling, leading to head rice yield (HRY) reductions. Minimizing kernel fissuring is thus an important goal of the rice industry. To minimize fissuring during the drying process, a better understanding of the fissuring process and the kinetics thereof is required. The goal of this research was to use X-ray imaging and glass transition principles to better understand rough rice kernel fissuring. First, because the instance when the fissuring process is initiated and completed during the drying process is not well known, an X-ray imaging setup that allows …

Association Mapping For Soybean (Glycine Max L. Merr.) Protein And Oil Content, Joseph Najjar

Graduate Theses and Dissertations

Soybean [Glycine Max (L.) Merrill] is an important economic crop because of its high content of seed protein and oil. Seed oil and protein content are quantitative inherited traits. The genetics of seed protein and oil levels have been extensively studied, with 367 QTL reported for protein and 475 QTL reported for oil to date. Validation of such QTLs, and identification of easily-automatable molecular markers around these QTL will aid the progression of breeding for such traits. The focus of this research was to discover novel and verify previously reported QTL related to protein and/or oil content via Genome-Wide Association …

Comparison Of A T-Trellis And A Rotating Cross-Arm Trellis For Arkansas Blackberry Production, Erika Henderson

Graduate Theses and Dissertations

As blackberry production in the southeastern United States expands, the need for updated cultural practices, like alternative trellis systems that enhance blackberry fruit yield, fruit quality, and economic viability for fresh-market growers are needed. There are challenges to producing high-quality blackberry fruit with high yields in the Southeast due to intense heat, high rainfall, humidity, and heavy pest pressure. Southeastern growers are interested in the rotating cross-arm (RCA) trellis due to its potential to increase yield and fruit quality, however there is minimal research of its use in blackberry production in the Southeast. In 2019 and 2020, a trial was …

Rice Chilling Aeration: Assessment Of Impacts On Grain Quality, Process Modeling And Validation, Soraya Shafiekhani

Graduate Theses and Dissertations

The goal of this study is to demonstrate the effectiveness of applying chilling temperatures during on-farm, in-bin drying and storage to maintain rice quality. Specific objectives include to demonstrate the effectiveness of chilling aeration to preserve rice quality characteristics, determine the drying kinetics of rice under low temperature environments, and develop models to predict heat and mass transfer in rice kernel during drying at low temperature environment. To date, there is limited research describing storability or drying behavior of rice in chilled environments. However, it is understood that chilling of high-moisture, > 21% wet basis rough rice could reduce the time …

Improving Breeding Program Efficiency And Genetic Gain Through The Implementation Of Genomic Selection In Diverse Wheat Germplasm, Dylan Larkin

Graduate Theses and Dissertations

Genomic selection (GS) is an important tool for increasing genetic gain for economically important traits in breeding programs. Genomic selection uses molecular markers across the entire genome in order to predict the performance of breeding lines for a trait of interest prior to phenotyping. A training population (TP) of elite germplasm, representative of the University of Arkansas wheat breeding program, was developed in order to predict important agronomic and Fusarium head blight (FHB) resistance traits within the University of Arkansas wheat breeding program through cross-validation and forward prediction.

A genome-wide association study (GWAS) was performed on the TP to identify …

Molecular Genetic Analysis Of Drought Resistance And Productivity Traits Of Rice Genotypes, Yheni Dwiningsih

Graduate Theses and Dissertations

Rice (Oryza sativa L.) is the staple food for a majority of the world’s population, and uses 30% of the global fresh water during its life cycle. Drought at the reproductive stage is the most important abiotic stress factor limiting grain yield. The United States is the third largest exporter of rice, and Arkansas is the top rice-producing state. The Arkansas rice-growing region in the Lower Mississippi belt is among the 10 areas with the highest risk of water scarcity. Adapted U.S. rice cultivars were screened for drought resistant (DR) traits to find sources for breeding U.S. rice cultivars for …

Influence Of Burning And Grazing Management Practices On Subirrigated Sandhill Meadow Hay Production, Tara M. Harms

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Subirrigated meadows are a valuable forage resource to Sandhills ranching operations being used for hay production, grazing, or a combination of both. Practices that sustain meadow productivity should be encouraged to ensure a consistent feed supply for cattle. The potential influence of prescribed burning or pre-freeze and post-freeze grazing on forage production and quality are not well understood on these meadows. In grasslands, including meadows, excess dead plant material can accumulate, causing a potential reduction in forage yield and quality. Results of our three-year field study suggest that burning meadows in the spring is a suitable management option to remove …

Evaluating The Impact Of Grafting On Local Tomato Production In Nebraska, Raihanah Hassim

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Vegetable grafting has been known to improve plant production under both biotic and abiotic stresses. With an increase in interest among local growers towards grafting production, it is important to provide enough vegetable grafting information. Therefore, the objective of this study is to assess the impact of grafting, rootstock cultivar, and local conditions and management on the yield and quality of tomato across the diverse growing and environmental conditions, specifically in Nebraska. Three open-field and one limited growing condition study were conducted between 2018 and 2019 across Nebraska. In the open-field trial, two determinant fresh market tomatoes, ‘Nebraska Wedding’ and …

Critical Period Of Palmer Amaranth Removal And Effects Of Late Season Herbicide Applications On Palmer Amaranth Seed Production, Jose De Sanctis

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Palmer amaranth is the most troublesome weed in agronomic crops in United States. Therefore, an integrated weed management approach is necessary to successfully manage this weed. The use of residual pre-emergence (PRE) herbicide applied at planting can delays the is one of the critical time of Palmer amaranth removal (CTPAR) giving farmers more time to prepare the weed control tactics and to preventing an unacceptable yield loss in soybean due to Palmer amaranth competition. Field experiments were conducted in 2018 and 2019 in a grower’s field infested with GR Palmer amaranth near Carleton, Nebraska, to determine the CTPAR in soybean …

Corn And Soybean Response To Wastewater-Recycled Phosphorus Fertilizers, Shane Ylagan

Crop, Soil and Environmental Sciences Undergraduate Honors Theses

The ability to recycle phosphorus (P) from wastewaters could provide a sustainable, continuous source of P that might also help protect surface water quality from P enrichment. The mineral struvite (MgNH4PO4·6H2O) is an understudied material that can be created from Pcontaining wastewater and has been shown to have agricultural fertilizer value. The objective of this study was to evaluate the effects of electrochemically precipitated struvite (ECST), chemically precipitated struvite (Crystal Green; CG), diammonium phosphate (DAP), monoammonium phosphate (MAP), rock phosphate (RP), and triple super phosphate (TSP) on corn (Zea mays) and soybean (Glycine max) response in a 79-day greenhouse pot …

Evaluating The Untapped Potential Of U.S. Conservation Investments To Improve Soil And Environmental Health, Andrea Basche, Katherine Tully, Nora L. Alvarez-Berrios, Julian Reyes, Laura Lengnick, Tabitha Brown, Jennifer M. Moore, Rachel E. Schattman, Lana Koepke Johnson, Gabrielle Roesch-Mcnally

Department of Agronomy and Horticulture: Faculty Publications

There is increasing enthusiasm around the concept of soil health, and as a result, new public and private initiatives are being developed to increase soil health-related practices on working lands in the United States. In addition, billions of U.S. public dollars are dedicated annually toward soil conservation programs, and yet, it is not well quantified how investment in conservation programs improve soil health and, more broadly, environmental health. The Environmental Quality Incentives Program (EQIP) is one of the major U.S. public conservation programs administered on privately managed lands for which public data are available. In this research, we developed a …

Polerovirus Genomic Variation And Mechanisms Of Silencing Suppression By P0 Protein, Natalie Holste

School of Biological Sciences: Dissertations, Theses, and Student Research

The family Luteoviridae consists of three genera: Luteovirus, Enamovirus, and Polerovirus. The genus Polerovirus contains 32 virus species. All are transmitted by aphids and can infect a wide variety of crops from cereals and wheat to cucurbits and peppers. However, little is known about how this wide range of hosts and vectors developed. In poleroviruses, aphid transmission and virion formation is mediated by the coat protein read-through domain (CPRT) while silencing suppression and phloem limitation is mediated by Protein 0 (P0)—a protein unique to poleroviruses. P0 gives poleroviruses a great advantage amongst plant viruses and diversifies polerovirus species, but the …

Cost Estimation Of Wastage Irrigation Water In Arid Public Landscape Areas King Abdullah Park– Dammam, Saudi Arabia, Ali Al-Sulbi

Emirates Journal for Engineering Research

The Kingdom of Saudi Arabia (KSA) locates within the territory of tropical deserts where water is one of the most important natural resources. However considerable amount of water has been wasted, on daily bases, in irrigation of public landscaped areas managed by local municipalities. Water wastage related to issues include improper calculation or no calculation of plants water budget; malfunctionality or failure of irrigation system components, tools and equipment; misunderstanding of soil-water and plant relationships; and improper management of irrigation operations. This research aims to estimate the cost of wasted water in association with irrigation of public landscape areas in …

Reducing Mowing Requirements In Home Lawn And Golf Course Turfgrass, Mark A. Keck

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Turfgrass systems are routinely managed by frequent mowing to increase aesthetics and function. Mowing is resource intensive with a high labor and energy demand. Reducing the number of mowings events in a growing season will decrease the labor and energy but may reduce quality as well. Previous work has looked at reducing mowing by changing the frequency and by using a plant growth regulator (PGR). Limited information is available about how to reduce mowing while maintaining acceptable quality. We looked at two different management practices to reduce mowing and maintain quality. The first study evaluated seven different mowing frequencies at …

Use Of An Active Canopy Sensor Mounted On An Unmanned Aerial Vehicle To Monitor The Growth And Nitrogen Status Of Winter Wheat, Jie Jiang, Zeyu Zhang, Qiang Cao, Yan Liang, Brian Krienke, Yongchao Tian, Yan Zhu, Weixing Cao, Xiaojun Liu

Department of Agronomy and Horticulture: Faculty Publications

Using remote sensing to rapidly acquire large-area crop growth information (e.g., shoot biomass, nitrogen status) is an urgent demand for modern crop production; unmanned aerial vehicle (UAV) acts as an effective monitoring platform. In order to improve the practicability and efficiency of UAV based monitoring technique, four field experiments involving different nitrogen (N) rates (0–360 kg N ha⁻¹ ) and seven winter wheat (Triticum aestivum L.) varieties were conducted at different eco-sites (Sihong, Rugao, and Xinghua) during 2015–2019. A multispectral active canopy sensor (RapidSCAN CS-45; Holland Scientific Inc., Lincoln, NE, USA) mounted on a multirotor UAV platform was used …