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Full-Text Articles in Life Sciences

Supplemental Effects Of Ruminal Bypass Arginine And Lysine For Improving Meat Quality And Oxidative Stability Of Aged Beef Loins, Jacob Tuell, Hyun-Wook Kim, Juliana Guedes, Derico Setyabrata, Jin-Kyu Seo, Jon Schoonmaker, Yuan H. Brad Kim Aug 2017

Supplemental Effects Of Ruminal Bypass Arginine And Lysine For Improving Meat Quality And Oxidative Stability Of Aged Beef Loins, Jacob Tuell, Hyun-Wook Kim, Juliana Guedes, Derico Setyabrata, Jin-Kyu Seo, Jon Schoonmaker, Yuan H. Brad Kim

The Summer Undergraduate Research Fellowship (SURF) Symposium

Postmortem aging is widely practiced in the beef industry to improve eating quality characteristics. However, the oxidative stability of aged beef muscle could be negatively affected by extending aging period. The use of ruminal bypass amino acids allows levels of crude protein in the diet to be lowered and may also positively impact oxidative stability of aged beef muscle. This study was conducted to evaluate the effects of ruminal bypass arginine (Arg) and lysine (Lys) supplementation on meat quality and oxidative stability of beef loins (M. longissimus lumborum, LL) under two durations of postmortem aging (14 and 28 days). …


Lysine Bioavailability Of Two Lipid Coated Lysine Products After Exposure To Silages With Different Acidity, Jessica N. Reiners Jan 2017

Lysine Bioavailability Of Two Lipid Coated Lysine Products After Exposure To Silages With Different Acidity, Jessica N. Reiners

Electronic Theses and Dissertations

Three experiments were conducted to determine bioavailability of 2 lipid coated Lys products (EB and EC). In an initial experiment, Lys disassociation from 2 lipid coated Lys products was best described by a first-order kinetic model after incubation in alfalfa- or corn-silage at 2 different amounts of acidity. Greater amounts of Lys immediately disassociated from EC (15.7%) than EB (4.9%; P < 0.05). Lysine that initially disassociated in corn silage at low pH (0.5%) was less than corn silage at a more neutral pH (18.7%), whereas Lys that initially disassociated in alfalfa silage was not affected (P = 0.26) by pH. Lysine that slowly disassociated from lipid coated Lys differed by product (P = 0.01), and acidity (P < 0.01) affected amounts of Lys that slowly disassociated differently within alfalfa- or corn-silage (pH × silage = 0.02). Greater amounts of Lys disassociated from acidic corn silage (79%) than neutral corn silage (32%; P = 0.01). However, amounts of Lys that slowly disassociated from alfalfa silage was not impacted by acidity (P = 0.40). Additionally, amounts of Lys that did not disassociate (P < 0.04) within 24 h were greater (P < 0.04) when lipid coated Lys was mixed with neutral silages (44%) compared to acidic silages (21%). However, rate of Lys disassociation from lipid coated Lys products was not affected by acidity or exposure to alfalfa- or corn-silage (P = 0.21). In a second experiment, we measured in vitro ammonia release from EB and EC after mixing with alfalfa- or corn-silage at different amounts of acidity with or without monensin. Ammonia release from Lys increased (Quadratic < 0.01) with greater amounts of time; however, differences between in vitro cultures with added Lys and negative control were not detected until 36 h of incubation. By 36 h, all cultures containing added Lys were greater than the negative control. After 42 h of incubation, ammonia release from Lys was greatest from EC and when lipid and Lys-HCl in amounts identical to EB were provided to cultures; EB, Lys-HCl, and lipid and Lys in amounts identical to EC were intermediate. After 48 h of incubation, ammonia release from Lys in EB was greatest and Lys-HCl was least, but EC and amounts of lipid and Lys-HCl identical to either EB or EC were intermediate. Ammonia release from Lys were less from cultures containing monensin after 48 h of incubation. In experiment 3, 9 white-faced ewes (70.1 ± 5.2 kg BW; 5.3 ± 0.6 yr) were used to measure Lys bioavailability by a slope-ratio analysis from 2 lipid coated Lys products and Lys-HCl after mixing in corn silage. Plasma Lys concentrations increased (Linear < 0.01) in response to abomasal infusion of Lys, and bioavailability of EB, EC, and Lys- HCl were calculated to be 10.74%, 18.82%, and 11.87%, respectively. However, increases in plasma Lys from EB (Linear = 0.41) and Lys-HCl (Linear = 0.36) were not different from plasma Lys supported by diet alone, but the rate of increase in plasma Lys in response to EC tended (Linear = 0.15) to be greater than plasma Lys levels from diet. Evidently, manufacturing method among lipid coated Lys products as well as physical and chemical characteristics of diet can impact availability of Lys from lipid coated Lys products.


Effects Of Amino Acid Ratios And Lysine Level On Nursery Pig Growth Performance, A. B. Clark, M. D. Tokach, J. M. Derouchey, S. S. Dritz, J. C. Woodworth, R. D. Goodband, K. J. Touchette Jan 2017

Effects Of Amino Acid Ratios And Lysine Level On Nursery Pig Growth Performance, A. B. Clark, M. D. Tokach, J. M. Derouchey, S. S. Dritz, J. C. Woodworth, R. D. Goodband, K. J. Touchette

Kansas Agricultural Experiment Station Research Reports

A total of 2,268 pigs (PIC 327 × L42, initially 16.2 lb BW) was used in a 28-d growth study with 54 pigs per feeder (experimental unit) and 6 replications per treatment. Pigs were randomly allotted to pens at weaning and fed a common starter diet for 8 d. Pens were then blocked by BW and allotted to 1 of 7 dietary treatments in a randomized complete block design. Treatments were arranged in two levels of standardized ileal digestible (SID) Lys (low,1.25% and high, 1.35%) and SID amino acid (AA) ratios relative to Lys (industry, 95% of maximum performance, and …


Effects Of Standardized Ileal Digestible Lysine On 15- To 25-Lb Nursery Pigs, G. E. Nichols, C. M. Vier, A. B. Clark, M. B. Menegat, H. S. Cemin, C. K. Jones, J. M. Derouchey, M. D. Tokach, R. D. Goodband, J. C. Woodworth, S. S. Dritz Jan 2017

Effects Of Standardized Ileal Digestible Lysine On 15- To 25-Lb Nursery Pigs, G. E. Nichols, C. M. Vier, A. B. Clark, M. B. Menegat, H. S. Cemin, C. K. Jones, J. M. Derouchey, M. D. Tokach, R. D. Goodband, J. C. Woodworth, S. S. Dritz

Kansas Agricultural Experiment Station Research Reports

A total of 300 pigs (DNA 241 × 600; initial pen average BW of 15.4 lb) were used in a 22-d growth trial to determine the standardized ileal digestible (SID) lysine (Lys) requirement of nursery pigs from 15- to 25-lb. Pigs were weaned at approximately 21 d of age and allotted to pens based on BW and gender. There were 10 replicate pens per treatment and 6 pigs per pen. Pigs were fed a common pelleted diet for 10 d post-weaning. Subsequently, pens of pigs were randomly assigned to 1 of 6 experimental diets in a randomized complete block design, …


Effects Of Increasing Dietary Lysine On Performance Of Lactating Sows In Commercial Conditions, K. M. Gourley, J. C. Woodworth, J. M. Derouchey, M. D. Tokach, S. S. Dritz, R. D. Goodband, S. J. Kitt, E. W. Stephenson Jan 2017

Effects Of Increasing Dietary Lysine On Performance Of Lactating Sows In Commercial Conditions, K. M. Gourley, J. C. Woodworth, J. M. Derouchey, M. D. Tokach, S. S. Dritz, R. D. Goodband, S. J. Kitt, E. W. Stephenson

Kansas Agricultural Experiment Station Research Reports

A total of 710 mixed parity sows (Line 241; DNA, Columbus, NE) were used in a 21-d study to determine the effect of standardized ileal digestible (SID) lysine (Lys) intake during lactation on sow and litter performance and subsequent reproductive performance of primiparous and multiparous sows housed in a commercial production system. On d 112 of gestation, females were weighed and blocked by BW within expected farrowing date and parity (1 to 7) and randomly assigned to 1 of 4 dietary treatments within blocks. Dietary treatments were corn-soybean meal-based and consisted of increasing SID Lys (0.75, 0.90, 1.05, and 1.20%). …